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1.
Theodoros Eleftheriadis Georgios Pissas Marta Crespo Evdokia Nikolaou Vassilios Liakopoulos Ioannis Stefanidis 《International journal of molecular sciences》2021,22(4)
Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection. 相似文献
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Samples removed from funeral figurines, dated the third to the second century BC (Hellenistic period) and found in Macedonia, Greece, are investigated using high‐performance liquid chromatography. Two results are reported that provide new insights into ancient polychromy. Firstly, high‐performance liquid chromatography results, in combination with historical information, indicate that insect species of cochineal, most probably Porphyrophora hamelii Brandt, was used in Hellenistic objects. Secondly, madder detected in the archaeological samples contains high amounts of purpurin (alizarin is either not detected or detected in trace). This relative composition, which is recorded using the hydrochloric acid method for dyestuff extraction, is consistent with that prepared from wild madder (Rubia peregrina L.), which is a common plant in the Mediterranean region. 相似文献
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Konstantinos Tserpes Ioannis Floros 《Fatigue & Fracture of Engineering Materials & Structures》2019,42(7):1430-1440
A numerical model has been developed for simulating fatigue crack growth (debonding) in adhesively bonded composite joints subjected to mode‐I, mode‐II, and mixed‐mode I + II loading conditions. The model employs a cohesive zone model described by a modified bilinear traction‐separation law. Fatigue damage in the composite adherends is not considered in the model. To account for crack divergence and reduce sensitivity of numerical results on mesh density, a crack front detection algorithm based on the effective element's length was employed. The model is implemented as a user‐defined subroutine (UMAT) in the commercial FE code LS‐DYNA. The model's input parameters, in the form of a modified Paris law, and the validation data were obtained from experimental tests conducted by the authors. It was found that the model is able to successfully simulate crack growth in the regime of the experimental data. 相似文献
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A novel MINLP-based representation of the original complex model for predicting gasoline emissions 总被引:1,自引:1,他引:0
The Environmental Protection Agency (EPA) introduced Reformulated Gasoline (RFG) requirements as a measure to reduce emissions from gasoline-powered vehicles in certain geographic areas. As part of this effort, the EPA developed empirical models for predicting emissions as a function of gasoline properties and established statutory baseline emissions from a representative set of gasolines. All reformulated gasoline requires certification via this model, known as the Complex Model, and all refiners and importers calculate emissions performance reductions from the statutory baseline gasoline. The current representation of the Complex Model is extremely difficult to implement within refinery operations models or to use in combination with models for designer gasoline. RFG and boutique fuels are key driving forces in the North American refining industry.The RFG models introduce increasingly complex constraints with the major limitation that they are implicitly defined through a series of complicated disjunctions assembled by the EPA in the form of spreadsheets. This implicit and cumbersome representation of the emissions predictive models renders rigorous optimization and sensitivity analysis very difficult to address directly. In this paper, we discuss how the federal government requirements for reformulated gasoline can be restated as a set of mixed-integer nonlinear programming (MINLP) constraints with the aid of disjunctive programming techniques. We illustrate the use of this model with two simple example fuel blending problems. 相似文献
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The embedding of a small but expressive language of multidimensional functional programming in a well known and widely used language of object‐oriented programming leads to the combination of two radically different programming models. In this paper, we formally define the syntax and semantics of GLU?, which can be thought of as the multidimensional core of Lucid and GLU, and we describe its implementation as a language embedded in C++. With the aid of a few examples, we argue that the marriage of the two programming models is not only compatible and natural, but also that it produces a new and interesting hybrid language. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
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Diana Hurdowar-Castro Ioannis Tsanis Ilmar Simanovskis 《Journal of Great Lakes research》2007,33(1):232-252
A 3D transport model is used to perform a comparative analysis of several potential drinking water intakes located along the northwest shore of Lake Ontario between Toronto and Oshawa. The model is specifically used to assess each intake under both long- and short-term transport of a potential pollutant release from the Pickering Nuclear Generating Station and potential and actual pollutant releases from local land sources respectively. A model based on a 500 m grid resolution is calibrated using data collected in the aftermath of the 1992 tritium spill at the Pickering Nuclear Generation Station and subsequently used to simulate long-term transport. A model based on a 100 m grid resolution is verified using drogue studies and used to simulate short-term transport events. Both models are used to assess pollutant levels at each of nine potential intake locations under different wind scenarios and pollutant releases. Field data for the study included water quality and flow measurements from local sewers and rivers, and estimates of pollutant levels from the local waste water treatment plants. This paper describes the model setup for both the long-term and short-term transport models, calibration using field data, long-term transport modeling, short-term transport modeling, and the comprehensive analysis approach used to evaluate the nine potential intake locations proposed. Results indicated that four intakes in particular outperformed other intake locations by maintaining bottom pollutant levels within governmental standards and warning times that exceeded 20 hours. 相似文献