QoS-Aware Optical Fog-Assisted Cyber-Physical System in the 5G Ready Heterogeneous Network

Wireless Personal Communications - In recent years, the emergence of Internet of things and cyber-physical system provide a proactive and efficacious solution to enable remote monitoring, machine...     

Identification of thermoresistive solar radiation sensors

An identification procedure to estimate the parameters of a thermoresistive solar radiation sensor is presented. The proposed technique employs only electrical excitation for the sensor. The estimation algorithm is recursive and is applied to the sensor model derived from the thermodynamic equilibrium differential equations. The simulation and the experimental results demonstrate the validity of the proposed approach     

Soluble Prokaryotic Overexpression and Purification of Human GM-CSF Using the Protein Disulfide Isomerase b′a′ Domain

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as neutrophils, dendritic cells, or macrophages. These proteins have thus generated considerable interest in clinical therapy research. A current obstacle to the prokaryotic production of human GM-CSF (hGM-CSF) is its low solubility when overexpressed and subsequent complex refolding processes. In our present study, the solubility of hGM-CSF was examined when combined with three N-terminal fusion tags in five E. coli strains at three different expression temperatures. In the five E. coli strains BL21 (DE3), ClearColi BL21 (DE3), LOBSTR, SHuffle T7 and Origami2 (DE3), the hexahistidine-tagged hGM-CSF showed the best expression but was insoluble in all cases at each examined temperature. Tagging with the maltose-binding protein (MBP) and the b′a′ domain of protein disulfide isomerase (PDIb′a′) greatly improved the soluble overexpression of hGM-CSF at 30 °C and 18 °C. The solubility was not improved using the Origami2 (DE3) and SHuffle T7 strains that have been engineered for disulfide bond formation. Two conventional chromatographic steps were used to purify hGM-CSF from the overexpressed PDIb′a′-hGM-CSF produced in ClearColi BL21 (DE3). In the experiment, 0.65 mg of hGM-CSF was isolated from a 0.5 L flask culture of these E. coli and showed a 98% purity by SDS-PAGE analysis and silver staining. The bioactivity of this purified hGM-CSF was measured at an EC₅₀ of 16.4 ± 2 pM by a CCK8 assay in TF-1 human erythroleukemia cells.     

A study on the deposition of pyrolytic carbons from hydrocarbons

A new method for forming isotropic, laminar, and columnar pyrolytic carbons is proposed. For this, a low RPM (below 2.4 rpm) tumbling bed has been used to deposit pyrolytic carbons from hydrocarbon gases. All deposits were made on graphite substrates from propane and methane at a constant temperature of 1200°C. The microstructures of the pyrolytic carbons deposited were dependent on the flow pattern of the reactant gas, the rpm of the reactor, the hydrocarbon concentration, the nature of the hydrocarbon, and the geometry of the bed. Isotropic pyrolytic carbon is formed under deposition conditions where homogeneous nucleation occurs in the gas phase and at the gas flow conditions where the gas-borne droplets can collide on the substrate. Laminar carbon is formed under deposition conditions where homogeneous nucleation does not occur in the gas phase and at gas flow conditions where the carbon species existing in the bulk of the gas phase can collide on the substrate. Columnar carbon is formed when any carbon products existing in the bulk of the gas phase cannot collide on the substrate. The suggested deposition mechanism can also be applied to pyrolytic carbons deposited in a fluidized bed or in a stationary bed. In particular, isotropic carbon can be obtained even in a stationary bed if the requirements for the deposition of the isotropic carbon described above are satisfied.     

Cloud based intelligent system for delivering health care as a service

The promising potential of cloud computing and its convergence with technologies such as mobile computing, wireless networks, sensor technologies allows for creation and delivery of newer type of cloud services. In this paper, we advocate the use of cloud computing for the creation and management of cloud based health care services. As a representative case study, we design a Cloud Based Intelligent Health Care Service (CBIHCS) that performs real time monitoring of user health data for diagnosis of chronic illness such as diabetes. Advance body sensor components are utilized to gather user specific health data and store in cloud based storage repositories for subsequent analysis and classification. In addition, infrastructure level mechanisms are proposed to provide dynamic resource elasticity for CBIHCS. Experimental results demonstrate that classification accuracy of 92.59% is achieved with our prototype system and the predicted patterns of CPU usage offer better opportunities for adaptive resource elasticity.     

A novel layered Li [Li0.12NizMg0.32−zMn0.56]O2 cathode material for lithium-ion batteries

Layered Li[Li_0.12Ni_zMg_0.32−zMn_0.56]O₂ oxide cathodes containing lithium atoms in the transition metal layers were synthesized and characterized using X-ray diffraction (XRD), galvanostatic cycling, and differential scanning calorimetry (DSC). The Li[Li_0.12Ni_zMg_0.32−zMn_0.56]O₂ cathodes deliver a specific discharge capacity of about 190 mAh/g at room temperature and 236 mAh/g at 55 °C when cycled between 2.7 and 4.6 V versus Li/Li⁺. Excellent capacity retention and smooth potential profiles at room and elevated temperatures over extended cycles suggest that this material does not convert into a spinel structure.     

Enhanced surface morphologies of screen-printed carbon nanotube films by heat treatment and their field-emission properties

A heating process for obtaining free-standing carbon nanotube emitters is presented with the aim of improving field-emission properties from the screen-printed multiwalled carbon nanotube (MWCNT) films. Using an atmosphere with an optimum combination of nitrogen and air for heat treatment of CNT films, the CNT emitters can be made to protrude from the surface. This allows for a high emission current and the formation of very uniform emission sites without special surface treatment. The morphological change of the CNT film by this technique has eliminated additional processing steps, such as surface treatment which may result in secondary contamination and damage to the film. Despite its simplicity the process provides a high reproducibility in emission current density which makes the films suitable for practical applications.     

加强战略管理势在必行

战略管理是指对企业经营战略的制订、实施和控制进行的管理。目前国内涂料企业的战略意识淡薄、决策随意性大 ,提出企业领导应把主要精力放在战略的制订和实施上 ,建立健全相关制度的建议