Compact optical diagnostic device for isothermal nucleic acids amplification

We recently reported the successful use of the loop-mediated isothermal amplification (LAMP) reaction for hepatitis B virus (HBV) DNA amplification and its optimal primer design method. In this study, we report the development of an integrated isothermal device for both amplification and detection of targeted HBV DNA. It has two major components, a disposable polymethyl methacrylate (PMMA) micro-reactor and a temperature-regulated optical detection unit (base apparatus) for real-time monitoring of the turbidity changes due to the precipitation of DNA amplification by-product, magnesium pyrophosphate. We have established a correlation curve (R² = 0.99) between the concentration of pyrophosphate ions and the level of turbidity by using a simulated chemical reaction to evaluate the characteristics of our device. For the applications of rapid pathogens detection, we also have established a standard curve (R² = 0.96) by using LAMP reaction with a standard template in our device. Moreover, we also have successfully used the device on seven clinical serum specimens where HBV DNA levels have been confirmed by real-time PCR. The result indicates that different amounts of HBV DNA can be successfully detected by using this device within 1 h.     

Isolated nonnegative solutions of infinite-dimensional algebraic Riccati equations

For an infinite-dimensional continuous (or discrete)-time linear system, based on the study of the representation of nonnegative solutions of the algebraic Riccati equation (ARE), we get some sufficient and necessary conditions for a nonnegative solution of (ARE) to be isolated in the set of all nonnegative solution of (ARE) with respect to the norm topology, the strong operator topology and weak operator topology, respectively.     

一种面向HDFS的多层索引技术

SOH（SQL over HDFS）系统通常将数据存储于分布式文件系统HDFS中,采用Map/Reduce或分布式查询引擎来处理查询任务。得益于HDFS以及Map/Reduce的容错能力和可扩展性,SOH系统可以很好地应对数据规模的飞速增长,完成分析型查询处理。然而,在处理选择型查询或交互式查询时,这类系统暴露出性能上的缺陷。本文提出一个通用的索引技术,可以应用于SOH系统中,以提高其查询处理的效率。分析了SOH系统访问HDFS文件的过程,指出了其中影响数据加载时间的关键因素;提出了split层和split内部双层索引机制;设计并实现了聚集索引和非聚集索引。最后,在标准数据集上进行了大量实验,并与现有基于HDFS的索引技术进行了比较。实验结果表明,所提出的索引技术可以有效地提高查询处理的效率。     

在超（超）临界机组中应用的新钢种

介绍了超（超）临界机组高效节能的特点,从钢种分类、发展脉络、应用范围、焊接特点等方面对应用于超（超）临界机组中的新钢种进行了论述。指出,超（超）临界机组是火力发电发展的新方向,发展高效率的火力发电机组的关键技术之一是开发耐高温性能更好的耐热钢,使其能用于更高的温度区间。     

Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos

After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos.     

Estimations of previewed road curvatures and vehicular motion by a vision-based data fusion scheme

In this study, a new framework of vision-based estimation is developed using some data fusion schemes to obtain previewed road curvatures and vehicular motion states based on the scene viewed from an in-vehicle camera. The previewed curvatures are necessary for the guidance of an automatically steering vehicle, and the desired vehicular motion variables, including lateral deviation, heading angle, yaw rate, and sideslip angle, are also required for proper control of the vehicular lateral motion via steering. In this framework, physical relationships of previewed curvatures among consecutive images, motion variables in terms of image features searched at various levels in the image plane, and dynamic correlation among vehicular motion variables are derived as bases of data fusion to enhance the accuracy of estimation. The vision-based measurement errors are analyzed to determine the fusion gains based on the technique of a Kalman filter such that the measurements from the image plane and predictions of physical models can be properly integrated to obtain reliable estimations. Off-line experimental works using real road scenes are performed to verify the whole framework for image sensing.     

RISC32-E: Field programmable gate array based sensor node with queue system to support fast encryption in Industrial Internet of Things applications

Industrial Internet of Things (IIoT) is an emerging technology that relies on the use of massively connected sensor nodes to gather industrial-related data. The collected data are used for postanalysis to generate insights for reducing production down time, cost optimization, and predictive maintenance. One of the key requirements for sensor node in such application is the data confidentiality; as such sensor data may potentially leak the manufacturing and industrial secret to their competitors. In this paper, a field programmable gate array (FPGA)-based sensor node with Advanced Encryption Standard (AES) crypto-processor is proposed to safeguard the sensor data. A novel queue system is proposed to further reduce the data processing time and energy consumption. The proposed queue system is able to achieve 1.48× speed up and ∼16% energy reduction, which makes it a competitive candidate for Industrial IoT applications. The technique developed in this paper can also be extended to implement FPGA-based gateway with encryption feature, which is very useful for edge computing in IoT applications.     

基于纳什均衡的智能合约缺陷检测

本文研究区块链智能合约的缺陷检测问题,即检测合约中是否存在部分合约方无论选择什么动作,均无法避免损失的状态.将智能合约问题转换成合约状态迁移图上的博弈策略选择问题,提出了基于纳什均衡理论的合约缺陷自动检测方法,以及为了提高检测效率,提出了针对合约状态变迁图和博弈策略形式的一系列化简方法.最后,实现了一个智能合约建模、分...     

微晶合金磁芯材料在逆变电源中的应用

目前微晶合金软磁材料已制成各种各样的磁性器件以替代传统硅钢、铁氧体和坡莫合金等磁性材料,介绍了微晶合金材料的磁性能,并与传统磁性材料进行对比分析,在逆变电源中,尤其在焊机逆变电源主变设计,普遍采用微晶合金为主变压器铁芯,并逐渐应用于电力电子工业及电力技术领域中。     

活性炭纤维吸附氙气机理的研究

研究了不同基体的活性炭纤维对氙气的吸附性能,同时对活性炭纤维结构修饰后的性能进行研究.结果表明活性炭纤维对氙气的吸附量不是随表面积增大而增加,修饰后的活性炭纤维的微孔尺寸变窄,对氙气的吸附量有所增加,吸附前后活性炭纤维的结构未发生变化.