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Quantitative UPLC‐MS/MS analysis of chlorogenic acid derivatives in antioxidant fractionates from dandelion (Taraxacum officinale) root
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Owen Kenny Thomas J. Smyth Chandralal M. Hewage Nigel P. Brunton 《International Journal of Food Science & Technology》2015,50(3):766-773
While qualitative studies have identified chlorogenic acids in antioxidant extracts, particularly ethyl acetate‐derived extracts, of Taraxacum officinale, quantitative analysis of these phenolic compounds remains largely unreported for this species. In this study, bioactivity‐guided fractionation of an antioxidant crude ethyl acetate extract (DPPH = 295.481 ± 0.955 mg TE g?1 extract) from T. officinale root resulted in a number of reverse‐phase fractions that demonstrated high antioxidant activity (DPPH = 1058.733–1312.136 mg TE g?1 extract), stronger than that of the synthetic antioxidant Trolox®. UPLC‐MS/MS screening of these fractions for the presence of selected mono‐ and di‐caffeoylquinic acids revealed large quantities of 1,5‐dicaffeoylquinic acid present in several fractions (853.052–907.324 μg mg?1), respectively. Due to the antioxidant potency and high levels of 1,5‐dicaffeoylquinic acid observed in these fractions, it was concluded that specifically this chlorogenic acid derivative is a major contributor to the antioxidant efficacy of dandelion root. 相似文献
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IG Sumner A Vaughan R Eisenthal RW Pickersgill AJ Owen PW Goodenough 《Canadian Metallurgical Quarterly》1993,1164(3):243-251
Papaya proteinase omega (pp omega) has been purified from dried latex both by immunoaffinity and traditional methods. Kinetic analysis revealed that (1), the pp omega-catalysed hydrolysis of N-benzoyl-L-arginine p-nitroanilide (BApNA) has a lower specificity (kcat/Km) than the same reaction catalysed by papain; (2), the pp omega-catalysed hydrolysis of a tripeptide substrate having phenylalanine at the second position (S2-site) showed a more similar specificity to that catalysed by papain; (3), the significant difference between the two enzymes is that steady state kinetics with both L-BApNA and a tripeptide enables the identification in pp omega of other ionizations affecting binding. The active sites of papain and pp omega can therefore be distinguished by pH-dependence of kcat/Km. 相似文献
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Norris Fran H.; Murphy Arthur D.; Baker Charlene K.; Perilla Julia L.; Rodriguez Francisco Gutiérrez; Rodriguez José de Jesús Gutiérrez 《Canadian Metallurgical Quarterly》2003,112(4):646
Prevalence rates of trauma and posttraumatic stress disorder (PTSD) were estimated from a probability sample of 2,509 adults from 4 cities in Mexico. PTSD was assessed according to Diagnostic and Statistical Manual of Mental Disorders (American Psychiatric Association, 1994) criteria using the Composite International Diagnostic Interview (CIDI; WHO, 1997). Lifetime prevalence of exposure and PTSD were 76% and 11.2%, respectively. Risk for PTSD was highest in Oaxaca (the poorest city), persons of lower socioeconomic status, and women. Conditional risk for PTSD was highest following sexual violence, but nonsexual violence and traumatic bereavement had greater overall impact because of their frequency. Of lifetime cases, 62% became chronic; only 42% received medical or professional care. The research demonstrates the importance of expanding the epidemiologic research base on trauma to include developing countries around the world. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Examined the theory that chronic migraine headaches can lead to permanent changes in brain function in 71 female college students. Ss were 20 classic migraineurs, 27 common migraineurs, and 24 controls. No significant group differences were found on the Halstead Reitan Neuropsychological Test Battery and additional memory tests. Classic migraineurs had significantly higher scores than common migraineurs on the Hysteria and Paranoia scales of the Minnesota Multiphasic Personality Inventory (MMPI) and significantly higher scores than the controls on Hypochondriasis and Hysteria scales. Explanations for the lack of cognitive deficits in this group are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Emergency department status boards: user-evolved artefacts for inter- and intra-group coordination 总被引:1,自引:1,他引:0
Robert L. Wears Shawna J. Perry Stephanie Wilson Julia Galliers James Fone 《Cognition, Technology & Work》2007,9(3):163-170
Large, easily viewed status boards are commonly used in some healthcare settings such as emergency departments, operating
theaters, intensive care units, and inpatient wards. Because these artefacts were developed by front-line users, and have
little to no supervisory or regulatory control, they offer valuable insights into the theories of work and hazard held by
those users. Although the status boards case were locally developed over many years for within-group coordination, they have
also become useful for between-group coordination across organizational boundaries. In this paper, we compare and contrast
the use of such status boards in two disparate settings: a US emergency department, and a UK pediatric ward, and note striking
similarities in their form and usage, despite the large differences in setting. 相似文献
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Annie I. Anton Julia B. Eart Matthew W. Vail Neha Jain Carrie M. Gheen Jack M. Frink 《Security & Privacy, IEEE》2007,5(1):45-52
Healthcare institutions typically post their privacy practices online as privacy policy documents. We conducted a longitudinal study that examines the effects of HIPAA's enactment on a collection of privacy policy documents for a fixed set of organizations over a four-year period. We present our analysis of 24 healthcare privacy policy documents from nine healthcare Web sites, analyzed using goal mining, a content-analysis method that supports extraction of useful information about institutions' privacy practices from documents. We compare our results to our pre-HIPAA study of these same institutions' online privacy practices and evaluate their evolution in the presence of privacy laws 相似文献
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Art Owen 《Data mining and knowledge discovery》2003,7(1):101-113
Data squashing was introduced by W. DuMouchel, C. Volinsky, T. Johnson, C. Cortes, and D. Pregibon, in Proceedings of the 5th International Conference on KDD (1999). The idea is to scale data sets down to smaller representative samples instead of scaling up algorithms to very large data sets. They report success in learning model coefficients on squashed data. This paper presents a form of data squashing based on empirical likelihood. This method reweights a random sample of data to match certain expected values to the population. The computation required is a relatively easy convex optimization. There is also a theoretical basis to predict when it will and won't produce large gains. In a credit scoring example, empirical likelihood weighting also accelerates the rate at which coefficients are learned. We also investigate the extent to which these benefits translate into improved accuracy, and consider reweighting in conjunction with boosted decision trees. 相似文献