Na+ and/or Cl− Toxicities Determine Salt Sensitivity in Soybean (Glycine max (L.) Merr.), Mungbean (Vigna radiata (L.) R. Wilczek), Cowpea (Vigna unguiculata (L.) Walp.), and Common Bean (Phaseolus vulgaris L.)

Grain legumes are important crops, but they are salt sensitive. This research dissected the responses of four (sub)tropical grain legumes to ionic components (Na⁺ and/or Cl⁻) of salt stress. Soybean, mungbean, cowpea, and common bean were subjected to NaCl, Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and a “high cation” negative control for 57 days. Growth, leaf gas exchange, and tissue ion concentrations were assessed at different growing stages. For soybean, NaCl and Na⁺ salts impaired seed dry mass (30% of control), more so than Cl⁻ salts (60% of control). All treatments impaired mungbean growth, with NaCl and Cl⁻ salt treatments affecting seed dry mass the most (2% of control). For cowpea, NaCl had the greatest adverse impact on seed dry mass (20% of control), while Na⁺ salts and Cl⁻ salts had similar intermediate effects (~45% of control). For common bean, NaCl had the greatest adverse effect on seed dry mass (4% of control), while Na⁺ salts and Cl⁻ salts impaired seed dry mass to a lesser extent (~45% of control). NaCl and Na⁺ salts (without Cl⁻) affected the photosynthesis (P_n) of soybean more than Cl⁻ salts (without Na⁺) (50% of control), while the reverse was true for mungbean. Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and NaCl had similar adverse effects on P_n of cowpea and common bean (~70% of control). In conclusion, salt sensitivity is predominantly determined by Na⁺ toxicity in soybean, Cl⁻ toxicity in mungbean, and both Na⁺ and Cl⁻ toxicity in cowpea and common bean.     

Performance evaluation of dynamic probabilistic broadcasting for flooding in mobile ad hoc networks

In mobile ad hoc networks (MANETs), flooding is a required message dissemination technique for network-wide broadcast. The conventional blind flooding algorithm causes broadcast storm problem, a high number of unnecessary packet rebroadcasts thus resulting in high contention and packet collisions. This paper proposes a new probabilistic approach that dynamically fine-tunes the rebroadcasting probability of a node for routing request packets (RREQs) according to the number of neighbour nodes. We evaluate the performance of the proposed approach for the ad hoc on demand distance vector (AODV) routing protocol and compared against the blind flooding, fixed probabilistic and adjusted probabilistic flooding [L.M.M.M. Bani-Yassein, M. Ould-Khaoua et al., Performance analysis of adjusted probabilistic broadcasting in mobile ad hoc networks, International Journal of Wireless Information Networks 13(2) (2006) 127–140; M.B. Yassein, M.O. Khaoua et al., Improving route discovery in on-demand routing protocols using local topology information in MANETs, Proceedings of the ACM international workshop on Performance Monitoring, Measurement, and Evaluation of Heterogeneous Wireless and Wired Networks, Terromolinos, Spain, ACM Press, 2006, pp. 95–99.] approaches. The simulation results show that our proposed approach demonstrates better performance than blind flooding, fixed probabilistic and adjusted flooding approaches.     

The optimal solution for the flow of a fourth-grade fluid with partial slip

The steady flow of a non-Newtonian fluid when slippage between the plate and the fluid occurs is considered. The constitutive equations of the fluid are modeled for a fourth-grade non-Newtonian fluid with partial slip; they give rise to nonlinear boundary value problems. Analytical solutions are obtained using powerful analytic techniques for solving nonlinear problems, homotopy perturbation and optimal homotopy asymptotic methods. The results obtained are compared with the numerical results and it is shown that solutions exist for all values of the non-Newtonian parameters. The solutions valid for the no-slip condition for all values of the non-Newtonian parameters can be derived as special cases of the present analysis. Finally the solutions are discussed using a graphical approach.     

Influence of fine ceramic aggregates on the residual properties of concrete subjected to elevated temperature

The residual properties of concrete subjected to elevated temperature are of importance to assess the stability of the structure. This paper investigates the performance of concrete containing white ware ceramic sand exposed to elevated temperature. Concrete mixes containing 0%, 50%, and 100% ceramic sand were prepared. The specimen were exposed to elevated temperatures of 200°C, 500°C, and 800°C for a duration of 60 minutes. Their residual mechanical properties (compressive strength, split tensile strength), ultra sonic pulse velocity, and mass change for different cooling regimes were investigated and compared among specimen. The results showed that incorporation of ceramic sand in concrete mixes improved the resistance against elevated temperature of hardened concrete.     

Physics‐based search and optimization: Inspirations from nature

This paper presents a review of recently developed physics‐based search and optimization algorithms that have been inspired by natural phenomena. They include Big Bang–Big Crunch, black hole search, galaxy‐based search, artificial physics optimization, electromagnetism optimization, charged system search, colliding bodies optimization, and particle collision algorithm.     

Permeability of high-performance concrete subjected to elevated temperature (600 °C)

Permeability is one of the most important parameters to quantify the durability of high-performance concrete. Permeability is closely related with the spalling phenomenon in concrete at elevated temperature. This parameter is commonly measured on non-thermally damaged specimens. This paper presents the results of an experimental investigation carried out to study the effect of elevated temperature on the permeability of high-performance concrete. For this purpose, three types of concrete mixtures were prepared: (i) control high-performance concrete; (ii) high-performance concrete incorporating polypropylene fibres; and (iii) high-performance concrete made with lightweight aggregates. A heating–cooling cycle was applied on 160 × 320 mm, 110 × 220 mm, and 150 × 300 mm cylindrical specimens. The maximum test temperature was kept as either 200 or 600 °C. After the thermal treatment, 65 mm thick slices were cut from each cylinder and dried prior to being subjected to permeability test. Results of thermal gradients in the concrete specimens during the heating–cooling cycles, compressive strength, and splitting tensile strength of concrete mixtures are also presented here. A relationship between the thermal damage indicators and permeability is presented.     

Prediction of compressive strength of self-compacting concrete containing bottom ash using artificial neural networks

The paper presents a comparative performance of the models developed to predict 28 days compressive strengths using neural network techniques for data taken from literature (ANN-I) and data developed experimentally for SCC containing bottom ash as partial replacement of fine aggregates (ANN-II). The data used in the models are arranged in the format of six and eight input parameters that cover the contents of cement, sand, coarse aggregate, fly ash as partial replacement of cement, bottom ash as partial replacement of sand, water and water/powder ratio, superplasticizer dosage and an output parameter that is 28-days compressive strength and compressive strengths at 7 days, 28 days, 90 days and 365 days, respectively for ANN-I and ANN-II. The importance of different input parameters is also given for predicting the strengths at various ages using neural network. The model developed from literature data could be easily extended to the experimental data, with bottom ash as partial replacement of sand with some modifications.     

Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells

The small intestinal epithelium has an important role in nutrition, but also in drug absorption and metabolism. There are a few two-dimensional (2D) patient-derived induced pluripotent stem cell (iPSC)-based intestinal models enabling easy evaluation of transcellular transport. It is known that animal-derived components induce variation in the experimental outcomes. Therefore, we aimed to refine the differentiation protocol by using animal-free components. More specifically, we compared maturation of 2D-cultured iPCSs toward small intestinal epithelial cells when cultured either with or without serum, and either on Geltrex or on animal-free, recombinant laminin-based substrata. Differentiation status was characterized by qPCR, immunofluorescence imaging, and functionality assays. Our data suggest that differentiation toward definitive endoderm is more efficient without serum. Both collagen- and recombinant laminin-based coating supported differentiation of definitive endoderm, posterior definitive endoderm, and small intestinal epithelial cells from iPS-cells equally well. Small intestinal epithelial cells differentiated on recombinant laminin exhibited slightly more enterocyte specific cellular functionality than cells differentiated on Geltrex. Our data suggest that functional small intestinal epithelial cells can be generated from iPSCs in serum-free method on xeno-free substrata. This method is easily converted to an entirely xeno-free method.     

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

The frequency and severity of extreme climatic conditions such as drought, salinity, cold, and heat are increasing due to climate change. Moreover, in the field, plants are affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative to compare the effects of stress combinations on crop plants relative to individual stresses. This study investigated the differential regulation of physio-biochemical and metabolomics parameters in peanut (Arachis hypogaea L.) under individual (salt, drought, cold, and heat) and combined stress treatments using multivariate correlation analysis. The results showed that combined heat, salt, and drought stress compounds the stress effect of individual stresses. Combined stresses that included heat had the highest electrolyte leakage and lowest relative water content. Lipid peroxidation and chlorophyll contents did not significantly change under combined stresses. Biochemical parameters, such as free amino acids, polyphenol, starch, and sugars, significantly changed under combined stresses compared to individual stresses. Free amino acids increased under combined stresses that included heat; starch, sugars, and polyphenols increased under combined stresses that included drought; proline concentration increased under combined stresses that included salt. Metabolomics data that were obtained under different individual and combined stresses can be used to identify molecular phenotypes that are involved in the acclimation response of plants under changing abiotic stress conditions. Peanut metabolomics identified 160 metabolites, including amino acids, sugars, sugar alcohols, organic acids, fatty acids, sugar acids, and other organic compounds. Pathway enrichment analysis revealed that abiotic stresses significantly affected amino acid, amino sugar, and sugar metabolism. The stress treatments affected the metabolites that were associated with the tricarboxylic acid (TCA) and urea cycles and associated amino acid biosynthesis pathway intermediates. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and heatmap analysis identified potential marker metabolites (pinitol, malic acid, and xylopyranose) that were associated with abiotic stress combinations, which could be used in breeding efforts to develop peanut cultivars that are resilient to climate change. The study will also facilitate researchers to explore different stress indicators to identify resistant cultivars for future crop improvement programs.