时间的校验:应对渐进性疾病危机——为艾滋病和痴呆症患者而建的两座康复花园

设计是价值观的一种表达,它将生活中的希望和灵感融合到我们建造的工作、娱乐、学习和场所中去,设计也是我们应对疾病,理解环境如何改善不健康的生理、社会和心理影响的一种表达形式。以为艾滋病患者所建的纽约乔尔·施奈伯纪念园和为老年痴呆症患者所建的北卡罗莱纳州金斯山丰盛生命中心花园为例,阐释辅助渐进性疾病患者康复的花园设计方法,为园艺疗法的花园设计提供借鉴。     

Unstained and in vivo fluorescently stained bacterial nucleoids and plasmolysis observed by a new specimen preparation method for high-power light microscopy of metabolically active cells

Microscope slides were coated with a layer of gelatin, the thickness of the gelatin increasing linearly along the long axis. The bacterial suspension is applied to the dried gelatin and covered by a coverslip. The medium is absorbed by the gelatin and thus the cells applied against the coverslip. By this method, cultures of concentrations below 10⁸ cells/ml provide statistically relevant numbers for observation without prior concentration steps. It is easier to apply than the existing methods for the observation of bacterial nucleoids by phase contrast imaging. Because the cells are maintained in growing conditions the method is useful for the vital fluorescence DAPI-staining of various bacterial species and for observations of plasmolysis and its reversal at different physiological conditions and extracellular osmolalities. The previously generally assumed view that the plasmolytic changes of the cell morphology are immediate upon the hyperosmotic shock and are rapidly repaired when the cell is able to metabolize actively was confirmed; this is in contrast to some recent claims.