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1.
A 0.3% Au/Al2O3 catalyst prepared by the incipient wetness (IW) method was investigated in the continuous-flow liquid-phase glucose oxidation. Therefore, a continuous stirred tank reactor (CSTR) system equipped with an ultrasonic separator was used. The continuous-flow glucose oxidation was carried out at 40 °C, pH 9 and 1 bar oxygen partial pressure. Residence time and glucose concentration were varied. The IW gold catalyst showed very high activity and selectivity to gluconic acid within its 110 days of operation and, thus, an excellent long-term stability. Even after severe microbial contaminations of the catalyst, its activity could be completely restored by in situ regeneration with 2-propanol. 相似文献
2.
Hui Ma Klaus-Dieter Schewe Bernhard Thalheim Qing Wang 《Service Oriented Computing and Applications》2012,6(3):189-205
Large-scale service-oriented computing is based on the idea that services from various servers are combined into one distributed application. Referring to a collection of services on one server as a ??service cloud??, the problem investigated in this paper is to define formal high-level specifications of such distributed applications and to enable the location of suitable services for them. Based on the language-independent model of Abstract State Services (AS2s), which serves as a universal integrated model for data and software as services, we extend AS2s by high-level action schemes called ??plots?? as a means to specify permitted sequences of service operations. On these grounds, we develop a model for service mediators, that is, specifications of composed services in which service slots have to be filled by actual services, and investigate matching conditions for slots of mediators and services. For a services to match a slot in a mediator, a (generalised) projection of the mediator must comply with the plot of the service. Furthermore, the service must be semantically adequate, which requires the use of a service ontology. 相似文献
3.
Sabine Schulze Christin Neuber Stephanie Mller Ute Hempel Lorenz C. Hofbauer Klaus-Dieter Schaser Jens Pietzsch Stefan Rammelt 《International journal of molecular sciences》2022,23(23)
Bone in diabetes mellitus is characterized by an altered microarchitecture caused by abnormal metabolism of bone cells. Together with diabetic neuropathy, this is associated with serious complications including impaired bone healing culminating in complicated fractures and dislocations, especially in the lower extremities, so-called Charcot neuroarthropathy (CN). The underlying mechanisms are not yet fully understood, and treatment of CN is challenging. Several in vitro and in vivo investigations have suggested positive effects on bone regeneration by modifying biomaterials with sulfated glycosaminoglycans (sGAG). Recent findings described a beneficial effect of sGAG for bone healing in diabetic animal models compared to healthy animals. We therefore aimed at studying the effects of low- and high-sulfated hyaluronan derivatives on osteoclast markers as well as gene expression patterns of osteoclasts and osteoblasts from patients with diabetic CN compared to non-diabetic patients with arthritis at the foot and ankle. Exposure to sulfated hyaluronan (sHA) derivatives reduced the exaggerated calcium phosphate resorption as well as the expression of genes associated with bone resorption in both groups, but more pronounced in patients with CN. Moreover, sHA derivatives reduced the release of pro-inflammatory cytokines in osteoclasts of patients with CN. The effects of sHA on osteoblasts differed only marginally between patients with CN and non-diabetic patients with arthritis. These results suggest balancing effects of sHA on osteoclastic bone resorption parameters in diabetes. 相似文献
4.
Pradeep Bhartiya Neha Kaushik Linh N. Nguyen Sander Bekeschus Kai Masur Klaus-Dieter Weltmann Nagendra Kumar Kaushik Eun Ha Choi 《International journal of molecular sciences》2022,23(6)
Excess amounts of redox stress and failure to regulate homeostatic levels of reactive species are associated with several skin pathophysiologic conditions. Nonmalignant cells are assumed to cope better with higher reactive oxygen and nitrogen species (RONS) levels. However, the effect of periodic stress on this balance has not been investigated in fibroblasts in the field of plasma medicine. In this study, we aimed to investigate intrinsic changes with respect to cellular proliferation, cell cycle, and ability to neutralize the redox stress inside fibroblast cells following periodic redox stress in vitro. Soft jet plasma with air as feeding gas was used to generate plasma-activated medium (PAM) for inducing redox stress conditions. We assessed cellular viability, energetics, and cell cycle machinery under oxidative stress conditions at weeks 3, 6, 9, and 12. Fibroblasts retained their usual physiological properties until 6 weeks. Fibroblasts failed to overcome the redox stress induced by periodic PAM exposure after 6 weeks, indicating its threshold potential. Periodic stress above the threshold level led to alterations in fibroblast cellular processes. These include consistent increases in apoptosis, while RONS accumulation and cell cycle arrest were observed at the final stages. Currently, the use of NTP in clinical settings is limited due to a lack of knowledge about fibroblasts’ behavior in wound healing, scar formation, and other fibrotic disorders. Understanding fibroblasts’ physiology could help to utilize nonthermal plasma in redox-related skin diseases. Furthermore, these results provide new information about the threshold capacity of fibroblasts and an insight into the adaptation mechanism against periodic oxidative stress conditions in fibroblasts. 相似文献
5.
6.
A Method for the Quantitative Control of Solid Phase Reactions in the Peptide Synthesis A quantitative control-method of partial steps of a solid phase peptide synthesis is described, based on a hydrazinolytic cleavage of protected peptides from the solid support. A separation of the peptide hydrazides by gelchromatography on sephadex G 15 in dimethylformamide and a quantitative evaluation of the hydrazide-content in the elution fractions is reported, which permits the control of the Nα-deprotection step in the same way as the evaluation of the coupling yields of the desired sequence. The new method is discussed for the example of two solid phase syntheses of the pentapeptide N-tert.-butyl-oxycarbonyl-L -valyl-S-benzyl-L -cysteinyl-L -alanyl-L - leucyl-O-benzyl-L -tyrosine hydrazide. 相似文献
7.
8.
Boniol Frédéric Wiels Virginie Aït-Ameur Yamine Schewe Klaus-Dieter 《International Journal on Software Tools for Technology Transfer (STTT)》2017,19(2):133-140
International Journal on Software Tools for Technology Transfer - 相似文献
9.
This paper focuses on extensions of Abstract State Machines (ASMs) for the benefit of a refinement calculus in the area of
data-intensive applications, in particular data warehouses and on-line analytical processing. The extensions lead to a typed
version of ASMs and a more specific notion of strong data refinement, which incorporates the preservation of information content
by means of schema dominance. Each typed ASM can be effectively translated into an equivalent ordinary one. Providing typed
ASMs helps to exploit the existing logical formalisms used in data-intensive applications to define a ground model. Furthermore,
strong data refinement helps to set up a refinement-based development method that uses provably correct standard refinement
rules. 相似文献
10.
Klaus-Dieter Rinnen Dahv A.V. Kliner Richard N. Zare Winifred M. Huo 《Israel journal of chemistry》1989,29(4):369-382
The relationship between quantum state populations and ion signals in (2+1) resonance-enhanced multiphoton ionization (REMPI) detection of HD is investigated both experimentally, by measurements of hot-nozzle population distributions, and theoretically, by calculations of the two-photon E,F 1Σg+—X1Σg+ transition moments. The experimental and theoretical relative rotational cross-sections are in good agreement for v″ = 0 and v″ = 1, but differ for v″ = 2 and for the relative vibrational cross-sections. Arguments are presented that saturation of the E,F-X transition is the main cause of these discrepancies. In spite of the presence of saturation, this calibration gives confidence in determining the relative internal state populations of HD from its (2+1) REMPI spectrum. 相似文献