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This short paper focuses on some issues involved in the design of ubiquitous technologies and their integration within physical spaces. Current design methodologies and techniques do not explicitly consider the importance of gaining a full understanding of the human experience of space when designing technologies that will pervade and become an integral part of our physical environment. This paper is specifically focused on the importance of considering spaces as places, and of analysing those features of a place that are going to shape users interactions with technologically enhanced environments. It also examines the future implications that this approach may have on theoretical and methodological aspects of interaction design.
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Luigina CiolfiEmail: Fax: +353-61-202734 |
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Mara Di Giulio Viviana di Giacomo Emanuela Di Campli Soraya Di Bartolomeo Susi Zara Guido Pasquantonio Amelia Cataldi Luigina Cellini 《Journal of materials science. Materials in medicine》2013,24(8):1977-1983
This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample. 相似文献
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Luigina Sorbara Trefford Simpson Stephanie Duench Marc Schulze Desmond Fonn 《Contact lens & anterior eye》2007,30(1):53-59
PURPOSE: The primary objective was to compare measures of bulbar redness objectively using a photometric method with standard grading methods. Measures of redness were made on 24 participants wearing a silicone hydrogel contact lens in one eye for overnight wear. This report compares hyperaemia after 1 week of daily wear (baseline) with redness measured after 6 months of overnight wear. METHOD: A new method of objectively measuring bulbar conjunctival redness was performed using the Spectrascan650 Photometer by Photo Research under fixed illumination. Photometric measures in CIEu(*) chromaticity values involve the measurement of chromaticity, a physical analogue of redness, greenness and blueness in the image. This method was validated in Part 1 of the study using repeated measurements on the photographic CCLRU scale. In Part 2 of the study, the photographic grading scale (CCLRU) from 0 (none) to 100 (extreme) was used to make the comparison. RESULTS: Part 1 indicated that the photometer provides a repeatable and reliable measure of bulbar redness (CCC=0.989). A moderately strong and significant correlation was found between the CIEu(*) chromaticity values and the analogue data (R=0.795, p=0.000) at each measurement session (from baseline to 1 day, 1 week, and 1, 3 and 6 months of overnight wear). CONCLUSIONS: This new standardized and objective method of measuring bulbar redness has great potential to replace subjective grading scales, especially with multi-centre studies, where variability between investigators occurs. This method may also detect smaller changes between visits or between eyes. 相似文献
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Silvia Di Lodovico Firas Diban Paola Di Fermo Morena Petrini Antonella Fontana Mara Di Giulio Adriano Piattelli Simonetta DErcole Luigina Cellini 《International journal of molecular sciences》2022,23(13)
Innovative non-antibiotic compounds such as graphene oxide (GO) and light-emitting diodes (LEDs) may represent a valid strategy for managing chronic wound infections related to resistant pathogens. This study aimed to evaluate 630 nm LED and 880 nm LED ability to enhance the GO antimicrobial activity against Staphylococcus aureus- and Pseudomonas aeruginosa-resistant strains in a dual-species biofilm in the Lubbock chronic wound biofilm (LCWB) model. The effect of a 630 nm LED, alone or plus 5-aminolevulinic acid (ALAD)-mediated photodynamic therapy (PDT) (ALAD-PDT), or an 880 nm LED on the GO (50 mg/l) action was evaluated by determining the CFU/mg reductions, live/dead analysis, scanning electron microscope observation, and reactive oxygen species assay. Among the LCWBs, the best effect was obtained with GO irradiated with ALAD-PDT, with percentages of CFU/mg reduction up to 78.96% ± 0.21 and 95.17% ± 2.56 for S. aureus and P. aeruginosa, respectively. The microscope images showed a reduction in the cell number and viability when treated with GO + ALAD-PDT. In addition, increased ROS production was detected. No differences were recorded when GO was irradiated with an 880 nm LED versus GO alone. The obtained results suggest that treatment with GO irradiated with ALAD-PDT represents a valid, sustainable strategy to counteract the polymicrobial colonization of chronic wounds. 相似文献
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Marta Colletti Angela Galardi Evelina Miele Virginia Di Paolo Ida Russo Cristiano De Stefanis Rita De Vito Martina Rinelli Andrea Ciolfi Biagio De Angelis Angelica Zin Alessandro Guffanti Maria Cristina Digilio Antonio Novelli Rita Alaggio Giuseppe Maria Milano Angela Di Giannatale 《International journal of molecular sciences》2021,22(11)
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