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C Lupu CA Goodwin AD Westmuckett JJ Emeis MF Scully VV Kakkar F Lupu 《Canadian Metallurgical Quarterly》1997,17(11):2964-2974
Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor.factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC with caveolin, urokinase-type plasminogen activator receptor, and glycosphingolipids. The localization of TFPI in caveolae in resting endothelium is proved by double immunogold electron microscopy for TFPI and caveolin. After ultracentrifugation of rat lung or EC homogenates through density gradients of Nycodenz, TFPI was highly enriched at densities of 1.05 to 1.08 g/mL, together with caveolin and alkaline phosphatase. By ELISA, more than half of the cellular TFPI was detected in Triton X-100-insoluble extracts of EC. TFPI incorporates [1-3H]ethanolamine and is cleaved from the cell surface by phosphatidylinositol-phospholipase C, indicating a specific glycosylphosphatidylinositol-anchorage mechanism for TFPI in the plasma membrane. Clustering of TFPI and its localization in caveolae are dependent on the presence of cholesterol in the membrane. Agonist-induced stimulation of EC caused marked changes of distribution for both TFPI and caveolin at subcellular level, with subsequent increase of the cell surface-associated inhibitory activity toward tissue factor.factor VIIa. Our findings suggest that, beside their function in transcytosis, potocytosis, cell surface proteolysis, and regulation of signal transduction, caveolae also play a direct role in the regulation of EC anticoagulant properties. 相似文献
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Alberto Schaeffer-Filho Emil Lupu Morris Sloman 《Journal of Network and Systems Management》2015,23(3):753-793
Ubiquitous systems and applications involve interactions between multiple autonomous entities—for example, robots in a mobile ad-hoc network collaborating to achieve a goal, communications between teams of emergency workers involved in disaster relief operations or interactions between patients’ and healthcare workers’ mobile devices. We have previously proposed the Self-Managed Cell (SMC) as an architectural pattern for managing autonomous ubiquitous systems that comprise both hardware and software components and that implement policy-based adaptation strategies. We have also shown how basic management interactions between autonomous SMCs can be realised through exchanges of notifications and policies, to effectively program management and context-aware adaptations. We present here how autonomous SMCs can be composed and federated into complex structures through the systematic composition of interaction patterns. By composing simpler abstractions as building blocks of more complex interactions it is possible to leverage commonalities across the structural, control and communication views to manage a broad variety of composite autonomous systems including peer-to-peer collaborations, federations and aggregations with varying degrees of devolution of control. Although the approach is more broadly applicable, we focus on systems where declarative policies are used to specify adaptation and on context-aware ubiquitous systems that present some degree of autonomy in the physical world, such as body sensor networks and autonomous vehicles. Finally, we present a formalisation of our model that allows a rigorous verification of the properties satisfied by the SMC interactions before policies are deployed in physical devices. 相似文献
4.
Leonidas Lymberopoulos Emil Lupu Morris Sloman 《Journal of Network and Systems Management》2003,11(3):277-303
This paper presents a framework for specifying policies for the management of network services. Although policy-based management has been the subject of considerable research, proposed solutions are often restricted to condition-action rules, where conditions are matched against incoming traffic flows. This results in static policy configurations where manual intervention is required to cater for configuration changes and to enable policy deployment. The framework presented in this paper supports automated policy deployment and flexible event triggers to permit dynamic policy configuration. While current research focuses mostly on rules for low-level device configuration, significant challenges remain to be addressed in order to:a) provide policy specification and adaptation across different abstraction layers; and, b) provide tools and services for the engineering of policy-driven systems. In particular, this paper focuses on solutions for dynamic adaptation of policies in response to changes within the managed environment. Policy adaptation includes both dynamically changing policy parameters and reconfiguring the policy objects. Access control for network services is also discussed. 相似文献
5.
Charalambides M. Flegkas P. Pavlou G. Rubio-Loyola J. Bandara A.K. Lupu E.C. Russo A. Dulay N. Sloman M. 《Network and Service Management, IEEE Transactions on》2009,6(1):15-30
Policy-based management provides the ability to (re-)configure differentiated services networks so that desired Quality of Service (QoS) goals are achieved. This requires implementing network provisioning decisions, performing admission control, and adapting bandwidth allocation to emerging traffic demands. A policy-based approach facilitates flexibility and adaptability as policies can be dynamically changed without modifying the underlying implementation. However, inconsistencies may arise in the policy specification. In this paper we provide a comprehensive set of QoS policies for managing Differentiated Services (DiffServ) networks, and classify the possible conflicts that can arise between them. We demonstrate the use of Event Calculus and formal reasoning for the analysis of both static and dynamic conflicts in a semi-automated fashion. In addition, we present a conflict analysis tool that provides network administrators with a user-friendly environment for determining and resolving potential inconsistencies. The tool has been extensively tested with large numbers of policies over a range of conflict types. 相似文献
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A. S. Lupashko G. K. Dikusar O. F. Lupu 《Surface Engineering and Applied Electrochemistry》2007,43(2):131-135
Application of a convective method and a combined method employing a high-frequency current alongside convection for drying kernels of apricot stones is experimentally studied. The drying rate, the drying rate constants, and the duration of the first and second periods are determined. The effect of a high-frequency electromagnetic field on the kinetics of the drying is analyzed. 相似文献
9.
Liliana Olenic Gheorghe Mihailescu Stela Pruneanu Ioan Bratu Alexandru R. Biris Dan Lupu Grigore Sigartau Lucian B. Tudoran 《Particulate Science and Technology》2005,23(1):79-83
Gold colloidal nanoparticles (GNPs) were prepared by reducing a complex of Au+. Na3Au(SO3)2 with sodium citrate. The procedure provides control of the dimension of nanoparticles produced, which is a function of reaction time and concentration of reactants. The nanoparticles were separated by centrifugation and their characteristics and dimensions were studied using UV-Vis and IR spectroscopy and transmission electronic microscopy (TEM). 相似文献
10.
D. Lupu A. Biris E. Indrea N. Aldea R.V. Bucur M. Morariu 《International Journal of Hydrogen Energy》1983,8(10):797-799
The hydrogenation properties of the alloys of overall formula Mg2Ni1?xFex (x ? 0.37) have been studied. In this range of composition multi-phase alloys were obtained containing Mg2Ni, Mg and more or less finely dispersed Fe in different coordination as proved by the EXAFS technique and Mössbauer spectroscopy. There is no significant substitution of Ni by Fe atoms in the Mg2Ni lattice. Two or three plateau-pressures are observed on the pressure-composition isotherms of the hydrides with the heats of formation in the range ?18.4 to 20.4 kcal/mol H2 (?77 to ?85.4 kJ/mol H2). The hydrides of the Fe containing alloys show higher desorption rates of hydrogen compared to the pure Mg2Ni hydride. 相似文献