排序方式: 共有7条查询结果,搜索用时 15 毫秒
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Farah Perveen Mughal Ann Christina Bergmann Ha Uyen Buu Huynh Sarah Hyllekvist Jrgensen Inaam Mansha Meliha Kesmez Patrick Mark Schürch Alexandre Pierre Andr Theocharides Paul Robert Hansen Tina Friis Morten Orebo Holmstrm Evaldas Ciplys Rimantas Slibinskas Peter Hjrup Gunnar Houen Nicole Hartwig Trier 《International journal of molecular sciences》2022,23(12)
Myeloproliferative Neoplasms (MPNs) constitute a group of rare blood cancers that are characterized by mutations in bone marrow stem cells leading to the overproduction of erythrocytes, leukocytes, and thrombocytes. Mutations in calreticulin (CRT) genes may initiate MPNs, causing a novel variable polybasic stretch terminating in a common C-terminal sequence in the frameshifted CRT (CRTfs) proteins. Peptide antibodies to the mutated C-terminal are important reagents for research in the molecular mechanisms of MPNs and for the development of new diagnostic assays and therapies. In this study, eight peptide antibodies targeting the C-terminal of CRTfs were produced and characterised by modified enzyme-linked immunosorbent assays using resin-bound peptides. The antibodies reacted to two epitopes: CREACLQGWTE for SSI-HYB 385-01, 385-02, 385-03, 385-04, 385-07, 385-08, and 385-09 and CLQGWT for SSI-HYB 385-06. For the majority of antibodies, the residues Cys1, Trp9, and Glu11 were essential for reactivity. SSI-HYB 385-06, with the highest affinity, recognised recombinant CRTfs produced in yeast and the MARIMO cell line expressing CRTfs when examined in Western immunoblotting. Moreover, SSI-HYB 385-06 occasionally reacted to CRTfs from MPN patients when analysed by flow cytometry. The characterized antibodies may be used to understand the role of CRTfs in the pathogenesis of MPNs and to design and develop new diagnostic assays and therapeutic targets. 相似文献
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Meliha Oktav Bulut Cihan Devirenoğlu Lutfi Oksuz Ferhat Bozdogan Erdogan Teke 《纺织学会志》2013,104(8):828-841
In this study, corona plasma discharge was applied to desize polyvinyl alcohol (PVA) and starch on cotton fabrics. Plasma treated and non-treated samples were processed in various steps in a textile firm. The samples were tested to evaluate their weight loss, size dissolution, capillarity, dyeability, pilling resistance and strength values. The surface morphology and the chemical structures were examined by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy analyses. The experimental results showed that the plasma has positive effects on size removal, hydrophility and the pilling values of the PVA and also starch sized fabrics. 相似文献
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Sezgin Meliha Kern-Isberner Gabriele Beierle Christoph 《Annals of Mathematics and Artificial Intelligence》2021,89(10-11):1101-1131
Annals of Mathematics and Artificial Intelligence - Probability kinematics is a leading paradigm in probabilistic belief change. It is based on the idea that conditional beliefs should be... 相似文献
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Derya Ilem-Ozdemir Evren Gundogdu Meliha Ekinci Emre Ozgenc Makbule Asikoglu 《Drug development and industrial pharmacy》2015,41(9):1493-1498
The purpose of this work is to prepare a self-microemulsifying drug delivery system (SMEDDS) for risedronate sodium (RSD) and to compare the permeability with RSD solution. The solubility of RSD was determined in different vehicles. Phase diagrams were constructed to determine the optimum concentration of oil, surfactant, and cosurfactant. RSD SMEDDS was prepared by using a mixture of soybean oil, cremophor EL, span 80, and transcutol (2.02:7.72:23.27:61.74, w/w, respectively). The prepared RSD SMEDDS was characterized by droplet size value. In vitro Caco-2 cell permeability studies were performed for SMEDDS and solution of radioactive (99?mTc-labeled RSD) and nonradioactive RSD. The experimental results indicated that RSD SMEDDS has good stability and its droplet size is between 216.68?±?3.79 and 225.26?±?7.65 during stability time. In addition, RSD SMEDDS has higher permeability value than the RSD solution for both radioactive and nonradioactive experiments. The results illustrated the potential use of SMEDDS for delivery of poorly absorbed RSD. 相似文献
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In this study, woollen yarn was dyed with industrial rose pulp from Isparta, a process that may pose environmental risks. The woollen yarn was processed with Savinase 16L, a protease enzyme, then dyed with biomordants such as citric acid, tannic acid and acetic acid, as well as with metal mordants such as aluminium sulphate, aluminium potassium sulphate, ferrous sulphate and potassium dichromate. The results showed that the yarn pretreated with the protease enzyme exhibited increased dyeability and pilling values without excessive loss of weight or strength. Although different shades were obtained with biomordants, different colours were obtained with metal mordants. The analysis of the waste water and metal content of the final product showed compliance with the OEKO-TEX® Standard 100. 相似文献
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A xanthine biosensor was prepared by electrochemical immobilization of xanthine oxidize enzyme onto carbon paste electrode via entrapment of Bi3+. After the optimization of experimental parameters, analytical characteristics were investigated. Two linear ranges between 0.02 and 0.06 and 1–7.5 μM with the equation y?=?93.00x?+?0.12 and y?=?1.07x?+?18.03 with the correlation coefficients of R 2?=?0.9951 and R 2?=?0.9931, respectively, were obtained for this biosensing system. RSD value was calculated for 0.04 μM xanthine (n?=?5) and found as 3.84%. LOD and LOQ values were also calculated and revealed as 1.30?×?10?8 and 4.3?×?10?8 M, respectively. Then, this biosensor was applied for xanthine detection in real samples. As a sample treatment, only necessary dilutions were made. Four types of beverages including wine, energy drink, peach, and sour cherry juice were used for this purpose. Obtained recovery values demonstrate that this system is applicable for xanthine detection in real samples without needing any laborious sample pretreatment procedures. 相似文献
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