基于蛋白质组学技术研究秦川牛肉宰后贮藏过程中肌红蛋白含量及其衍生物转化

本实验采用4D-非标记蛋白质组学技术研究秦川牛肉贮藏过程中（0～8 d）肌红蛋白含量及其衍生物的转化情况，阐释冷却秦川牛肉中肌红蛋白含量及其衍生物转化的分子机制。结果表明：贮藏过程中，肌红蛋白表达量在宰后0～4 d上升、4～8 d下降，利用非标记蛋白质组学技术鉴定出与肌红蛋白及其衍生物相关的差异蛋白14 种，具体包括代谢酶、氧化还原酶、过氧化物酶、伴侣蛋白4 类，这4 类蛋白的表达共同调控贮藏过程中肌红蛋白含量的变化及其3 种衍生物之间的转化，具体表现为贮藏过程中肌红蛋白表达量整体呈下降趋势，氧合肌红蛋白相对含量持续下降，脱氧肌红蛋白、高铁肌红蛋白相对含量逐渐增加，导致肉色发生褐变。本研究结果有利于理解秦川牛肉贮藏过程肉类变色的复杂生化变化机制。     

Formal Analysis of Streaming Downloading Protocol for System Upgrading

For a PC-mobile download system which is embedded with streaming download protocol, there are problems that the data cannot be transmitted correctly from the PC to the mobile, or the transmission is unacceptably slow. To solve these problems, we carry out a formal analysis for the protocol with some timing parameters and a given probability of message loss and unordered data using a probabilistic model checking tool PRISM. We introduce a technique to reduce the state space of the system modeling the protocol which is a network of probabilistic timed automata. The experimental results in PRISM give us a clear explanation to the problems, and are helpful in identifying the optimal parameter settings to meet industrial requirements.     

南水北调东干渠工程安全监测数据分析与处理技术研究

东干渠工程是北京市南水北调配套工程之一,是中心城环线供水系统重要组成部分,承担第十水厂、通州水厂等东部区域供水任务.东干渠工程建设了安全监测系统,监测内容包括环境量、变形、渗流、应力、应变及专项监测等.但监测系统存在监测数据中断、仪器失效及缺少初值等问题.对某典型断面的应力、应变及温度等内观监测数据以及东五环段的沉降监测数据进行了梳理、对比验证分析,明确了监测数据的合理性,初步确定了沉降风险点,为东干渠工程安全监控提供技术支撑.     

OsMre11 Is Required for Mitosis during Rice Growth and Development

Meiotic recombination 11 (Mre11) is a relatively conserved nuclease in various species. Mre11 plays important roles in meiosis and DNA damage repair in yeast, humans and Arabidopsis, but little research has been done on mitotic DNA replication and repair in rice. Here, it was found that Mre11 was an extensively expressed gene among the various tissues and organs of rice, and loss-of-function of Mre11 resulted in severe defects of vegetative and reproductive growth, including dwarf plants, abnormally developed male and female gametes, and completely abortive seeds. The decreased number of cells in the apical meristem and the appearance of chromosomal fragments and bridges during the mitotic cell cycle in rice mre11 mutant roots revealed an essential role of OsMre11. Further research showed that DNA replication was suppressed, and a large number of DNA strand breaks occurred during the mitotic cell cycle of rice mre11 mutants. The expression of OsMre11 was up-regulated with the treatment of hydroxyurea and methyl methanesulfonate. Moreover, OsMre11 could form a complex with OsRad50 and OsNbs1, and they might function together in non-homologous end joining and homologous recombination repair pathways. These results indicated that OsMre11 plays vital roles in DNA replication and damage repair of the mitotic cell cycle, which ensure the development and fertility of rice by maintaining genome stability.     

Red-Blood-Cell-Membrane-Coated Metal-Drug Nanoparticles for Enhanced Chemotherapy

To overcome high toxicity, low bioavailability and poor water solubility of chemotherapeutics, a variety of drug carriers have been designed. However, most carriers are severely limited by low drug loading capacity and adverse side effects. Here, a new type of metal-drug nanoparticles (MDNs) was designed and synthesized. The MDNs self-assembled with Fe(III) ions and drug molecules through coordination, resulting in nanoparticles with high drug loading. To assist systemic delivery and prolong circulation time, the obtained MDNs were camouflaged with red blood cell (RBCs) membranes (RBCs@Fe-DOX MDNs) to improve their stability and dispersity. The RBCs@Fe-DOX MDNs presented pH-responsive release functionalities, resulting in drug release accelerated in acidic tumor microenvironments. The outstanding in vitro and in vivo antitumor therapeutic outcome was realized by RBCs@Fe-DOX MDNs. This study provides an innovative design guideline for chemotherapy and demonstrates the great capacity of nanomaterials in anticancer treatments.     

基于卷积神经网络的直线描述方法研究

为提高直线特征匹配的可靠性,提出一种基于卷积神经网络（CNN）学习的直线特征描述方法。构建用于网络学习的大规模直线数据集,该数据集包含约20.8万对匹配直线对,每条直线用其周围的局部图像块表征。将图像块输入CNN,利用HardNet网络结构提取特征,使用三元组损失函数进行训练,输出强鲁棒性的直线特征描述子。实验结果表明,与手工设计的描述子MSLD和IOCD相比,该描述子在视角、模糊、尺度和旋转变化下均具有较好的区分性,在图像拼接应用中同样表现出良好的描述性能。     

犹豫模糊语言后悔理论和ELECTRE决策方法及应用

针对决策信息为犹豫模糊语言元素形式、属性权重完全未知的多属性决策问题,提出了一种基于后悔理论和ELECTRE III的多属性决策方法。利用灰色关联分析和极大熵原理确定属性权重。确定犹豫模糊语言信息的后悔-欣喜函数,凭借该函数确定方案对的后悔-欣喜和谐指数与不和谐指数,进而确定方案对的可信度指数。通过方案对的可信度指数确定各方案的净可信度,依此对方案进行排序。通过算例说明了所提方法的可行性和有效性。由于该方法同时考虑了决策者的心理行为和属性间的部分可补偿性,因此决策结果更加贴近现实且更为合理。对后悔规避系数[μ]的灵敏度分析表明了所提方法的稳定性,与其他两种方法的对比分析展示了所提方法的优势。     

流动空气压差比拟与风电叶片翼型线确定理论的探讨

翼型是风电叶片设计的关键元素,对风电叶片的气动性能、风能转化和利用率都有重要影响。因此,翼型研究一直是风电叶片研究的热点和难点。论文深入分析了流动空气与风电叶片的相互作用,提出了用空气重力比拟其流动前后压差产生的动力的概念,建立了流动空气沿风电叶片流动的能量转化关系,以此为基础推导出了风电叶片翼型线理论公式,并用Xfoil软件对比分析了翼型线理论公式确定的新翼型和BRUXEL36翼型,发现在-2°~6°攻角条件下,新翼型具有较高升阻比,其中在攻角为3°时,新翼型升阻比高出BRUXEL36翼型25左右。论文研究为流动空气与风电叶片相互作用理论及风电叶片翼型线理论的发展提供了新尝试和重要借鉴。     

乌伦古坳陷断裂特征及其构造演化过程

利用现有二维地震资料进行断裂解释,识别乌伦古坳陷主断裂及其衍生断裂的的展布特征,分析断裂形成的力学背景。乌伦古东断裂和吐孜托依拉断裂是主要的控坳断裂,属于逆冲推覆类型的断裂;伦2井南断裂是以逆冲推覆为主、走滑为辅的断裂。根据乌伦古坳陷中生代沉降中心的演化特点,认为在印支和燕山期乌伦古坳陷东北缘所受挤压力强于西北缘。