Consensus-based data replication protocol for distributed cloud

The Journal of Supercomputing - Data availability ensures efficient data accessibility by the readers anytime and from anywhere. It can be addressed by creating multiple copies of each data file...     

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli

The Calvin Cycle is the primary conduit for the fixation of carbon dioxide into the biosphere; ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the rate-limiting fixation step. Our goal is to direct the evolution of RuBisCO variants with improved kinetic and biophysical properties. The Calvin Cycle was partially reconstructed in Escherichia coli; the engineered strain requires the Synechococcus PCC6301 RuBisCO for growth in minimal media supplemented with a pentose. We randomly mutated the gene encoding the large subunit of RuBisCO (rbcL), co-expressed the resulting library with the small subunit (rbcS) and the Synechococcus PCC7492 phosphoribulokinase (prkA), and selected hypermorphic variants. The RuBisCO variants that evolved during three rounds of random mutagenesis and selection were over-expressed, and exhibited 5-fold improvement in specific activity relative to the wild-type enzyme. These results demonstrate a new strategy for the artificial selection of RuBisCO and other non-native metabolic enzymes.     

Decouple and compare the role of abiotic factors and developed iron and sulphur oxidizers for enhanced extraction of metals from television printed circuit boards

Chemical leaching agents were compared for metal extractions from selected e-waste. Effect of iron, sulphur oxidizers and the formulated consortium were investigated for metals extraction from television printed circuit boards. The consortium showed maximum metal leaching efficiency. The bioleaching profile was studied as a function of pH, redox potential, acid consumed and the amount of Cu, Zn and Ni extracted. At 10 g/L of e-waste, 90%, 93% and 87% of Cu, Zn and Ni were extracted within 96 h. Ethylene diamine tetra acetic and pyrite were found beneficial. Two-step process resulted in more metal extraction even at 100 g/L pulp density.