Developmental and light-dependent changes of the cytosolic chaperonin containing TCP-1 (CCT) subunits in maize seedlings, and the localization in coleoptiles

The cytosolic chaperonin containing TCP-1 (CCT) is known to keep fold cytoskeletal proteins and is involved in the proper organization of the cytoskeleton. These studies are based on the assumption that growth responses linked to structural rearrangement of the plant cytoskeleton include the action of CCT and the need for newly synthesized tubulin. The presence of the alpha- and epsilon-subunits of CCT was investigated in soluble fractions of protein extracts from maize mesocotyls and coleoptiles at distinct growth stages. The CCT-subunits, tubulins and actin decreased in the coleoptile in response to far-red light. In addition, independent from light treatment, the amount of CCT epsilon abundance declined with age in coleoptiles and mesocotyls between 2 and 4.5 days after sowing. In contrast to CCT epsilon, no significant light regulation of CCT alpha was found in the mesocotyl. In two day old, light-grown rapidly elongating coleoptiles part of the CCT alpha subunit and the bulk of actin and tubulin was found shifted into fractions of high molecular weight complexes when compared to slowly elongating, dark grown coleoptiles. In 4.5 day old, etiolated and elongating coleoptiles, part of both CCT-subunits and cytoskeleton proteins were found in fractions of high molecular weight. A complete disappearance of these polypeptides was observed in old far-red irradiated growth-arrested coleoptiles. CCT epsilon was found to be colocalized to microtubular structures and to the nucleus. We conclude from our data that abundance of CCT-subunits in soluble extracts is dependent on age and light treatment, but independent from the growth stage of mesocotyl and coleoptile.     

Rainbow smelt population responses to species invasions and change in environmental condition

Invasive species can cause major disruptions in native food webs, yet the impact of species introductions and whether they will become invasive appears to be context-dependent. Rainbow smelt and alewife co-exist as invasive species in the Laurentian Great Lakes and as native species on the Atlantic coast of North America, but in Lake Champlain rainbow smelt is the dominant native forage fish and alewife are invasive. Alewife became abundant by 2007, providing an opportunity to explore the dynamics of these two species in a system where only one is invasive. We used data from a 28-year forage fish survey to compare demographics of rainbow smelt populations in three basins of Lake Champlain with different volumes, nutrient levels, and predator abundances. Rainbow smelt catch-per-unit-effort (CPUE) remained constant in the large, deep Main Lake before and after alewife invaded, but decreased in the two smaller basins. Declines were primarily a result of increased age-0 and age-1 mortality. Predation by top piscivores, system productivity, and competition for resources alone could not explain the patterns in CPUE across the basins. The mechanisms that allow alewife and rainbow smelt to co-exist could be related to system volume and oxythermal habitat availability, and may explain why the two species do not negatively affect each other in the Great Lakes. Summer hypoxia in the smaller basins could force individuals into smaller habitat volumes with higher densities of competitors and cannibalistic adult rainbow smelt. Habitat availability may mediate the impact of invasive alewife on native rainbow smelt.     

1993: Axline's eight principles of play therapy revisited.

Examined V. Axlines's book, Play Therapy (1947) which includes 8 basic principles on non-directive play therapy. Student responses to learning these principles in play therapy classes were also examined. Each student was assigned a play therapy client and was observed and assisted directly by the instructor, a licensed clinical psychologist. Clients were mildly trouble children (aged 5–10 yrs) and were selected from a referral base by the instructor as appropriate for the course. As the semester progressed and students gained direct experience, weekly small (3–4 student) group discussions were held to explore in-depth understanding of play therapy principles and how these principles are put into practice. It is concluded that Axline's book remains a classic text and a foremost resource in the field. Coupling it with experiential learning is an invaluable basis for teaching play therapy. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Embodied categorisation for vision-guided mobile robots

This paper outlines a philosophical and psycho-physiological basis for embodied perception, and develops a framework for conceptual embodiment of vision-guided robots. We argue that categorisation is important in all stages of robot vision. Further, that classical computer vision is unsuitable for this categorisation, however, through conceptual embodiment, active perception can be effective. We present a methodology for developing vision-guided robots that applies embodiment, explicitly and implicitly, in categorising visual data to facilitate efficient perception and action. Finally, we present systems developed using this methodology, and demonstrate that embodied categorisation can make algorithms more efficient and robust.     

RuleML representation and simulation of Fuzzy Cognitive Maps

Fuzzy Cognitive Map (FCM) technique is a combination of Fuzzy Logic and Artificial Neural Networks that is extensively used by experts and scientists of a diversity of disciplines, for strategic planning, decision making and predictions. A standardized representation of FCMs accompanied by a system that would assist decision makers to simulate their own developed Fuzzy Cognitive Maps would be highly appreciated by them, and would help the dissemination of FCMs. In this paper, (a) a RuleML representation of FCM is proposed and (b) a system is designed and implemented in Prolog programming language to assist experts to simulate their own FCMs. This system returns results in valid RuleML syntax, making them readily available to other cooperative systems. The representation capabilities and the design choices of the implemented system are discussed and a variety of examples are given to demonstrate the use of the system.     

Virtual Interpersonal Touch: Expressing and Recognizing Emotions Through Haptic Devices

ABSTRACT

This article examines the phenomenon of Virtual Interpersonal Touch (VIT), people touching one another via force-feedback haptic devices. As collaborative virtual environments become utilized more effectively, it is only natural that interactants will have the ability to touch one another. In the work presented here, we used relatively basic devices to begin to explore the expression of emotion through VIT. In Experiment 1, participants utilized a 2 DOF force-feedback joystick to express seven emotions. We examined various dimensions of the forces generated and subjective ratings of the difficulty of expressing those emotions. In Experiment 2, a separate group of participants attempted to recognize the recordings of emotions generated in Experiment 1. In Experiment 3, pairs of participants attempted to communicate the seven emotions using physical handshakes. Results indicated that humans were above chance when recognizing emotions via VIT but not as accurate as people expressing emotions through nonmediated handshakes. We discuss a theoretical framework for understanding emotions expressed through touch as well as the implications of the current findings for the utilization of VIT in human-computer interaction.