Developmental and light-dependent changes of the cytosolic chaperonin containing TCP-1 (CCT) subunits in maize seedlings, and the localization in coleoptiles

The cytosolic chaperonin containing TCP-1 (CCT) is known to keep fold cytoskeletal proteins and is involved in the proper organization of the cytoskeleton. These studies are based on the assumption that growth responses linked to structural rearrangement of the plant cytoskeleton include the action of CCT and the need for newly synthesized tubulin. The presence of the alpha- and epsilon-subunits of CCT was investigated in soluble fractions of protein extracts from maize mesocotyls and coleoptiles at distinct growth stages. The CCT-subunits, tubulins and actin decreased in the coleoptile in response to far-red light. In addition, independent from light treatment, the amount of CCT epsilon abundance declined with age in coleoptiles and mesocotyls between 2 and 4.5 days after sowing. In contrast to CCT epsilon, no significant light regulation of CCT alpha was found in the mesocotyl. In two day old, light-grown rapidly elongating coleoptiles part of the CCT alpha subunit and the bulk of actin and tubulin was found shifted into fractions of high molecular weight complexes when compared to slowly elongating, dark grown coleoptiles. In 4.5 day old, etiolated and elongating coleoptiles, part of both CCT-subunits and cytoskeleton proteins were found in fractions of high molecular weight. A complete disappearance of these polypeptides was observed in old far-red irradiated growth-arrested coleoptiles. CCT epsilon was found to be colocalized to microtubular structures and to the nucleus. We conclude from our data that abundance of CCT-subunits in soluble extracts is dependent on age and light treatment, but independent from the growth stage of mesocotyl and coleoptile.     

有助于防止激光二极管毁坏的激光仿真器

激光二极管可能在数纳秒内自行毁坏，因此测试一个反馈稳定的激光二极管驱动器的响应和稳定性可能是费用很高的。图1所示仿真器电路示出了一个典型的激光二极管封装，封装内不仅有由电流1．驱动的激光二极管，而且还有一个光电二极管。激光二极管的前端面发射在外界起作用的主光束，而后端面则发射落到光电二极管上的参考光束。     

Scalar network analysers operating at Ka- and W-bands

Scalar network analysers measuring the amplitude of the reflection/transmission coefficients of waveguide structures have been realised at Ka- and W-bands. Their accuracy derived by using standard mismatches is excellent at Ka- band and fairly good at W-band. To our knowledge this is the first instance where the accuracy of measurements obtained from a scalar network analyser was checked over full waveguide bands with the aid of standard mismatches. The reported reflectometers are precursors of vector network analysers based on six-port reflectometers. Methods aimed at increasing the signal-to-noise ratio (SNR) of the measurements have been developed, while proposals for improving the dynamic range of these network analysers are put forward.     

PAC-learnability of probabilistic deterministic finite state automata in terms of variation distance

We consider the problem of PAC-learning distributions over strings, represented by probabilistic deterministic finite automata (PDFAs). PDFAs are a probabilistic model for the generation of strings of symbols, that have been used in the context of speech and handwriting recognition, and bioinformatics. Recent work on learning PDFAs from random examples has used the KL-divergence as the error measure; here we use the variation distance. We build on recent work by Clark and Thollard, and show that the use of the variation distance allows simplifications to be made to the algorithms, and also a strengthening of the results; in particular that using the variation distance, we obtain polynomial sample size bounds that are independent of the expected length of strings.     

Using Automated Negotiation for Grid Services

The popularity of grid services has widened their application to numerous domains and increased the utilization of computational resources. In order to create more incentives for the resources owners to lease their resources and prevent users from wasting the resources, the introduction of a market-oriented grid is inevitable. However, the issues for the negotiation between service provider and consumer over the supply and demand of resources can be complex, with highly interdependent issues. In this research, a simulated automated negotiation mechanism including a co-evolutionary mechanism and a modified game theory approach is proposed, to assist them in reaching an agreement over the conflicting issues. In the proposed architecture, the co-evolution process is able to reduce the multiple dimensional search space into a two-dimension search space and identify the appropriate negotiation strategies for the negotiating agents to form a payoff matrix which can be used for the game theory related stage of their interaction. The multiple stage negotiation process is introduced to improve the negotiation result. In this paper, an application which requires a large amount of computational resources to process the data generated from mobile devises is used to demonstrate that the proposed system is able to resolve the conflicts and obtain a valid solution.     

The lipid composition of selected tissues from a Mediterranean monk seal,Monachus monachus

The lipid composition of blubber, brain, muscle and heart from a Mediterranean monk sealMonachus monachus (an endangered species) were examined to allow comparisons with more common species of seals. Only neutral lipids (mainly triacylglycerols) were detectable in the blubber lipids, whereas polar lipids predominated in the heart and in the brain. Neutral and polar lipids comprised almost equal proportions in both liver and muscle. Choline glycerophospholipids (CGP) were the major polar lipids, followed by ethanolamine glycerophospholipids (EGP) in the liver, heart and muscle. Cerebrosides accounted for 28.8% of the brain lipids. All lipid classes of the liver contained high levels (31–47%) of polyunsaturated fatty acids (PUFA), with the exception of phosphatidylserine. The total proportion of n−6 PUFA exceeded that of n−3 PUFA in all lipid classes of the liver, due mainly to the high levels of 20∶4n−6. The highest level of 20∶4n−6 occurred in phosphatidylinositol, where it comprised 32.4% of the total fatty acids. The CGP and EGP of the brain contained lower levels of PUFA than those of the liver, muscle and heart. Alkenyl ethers accounted for 35.8% of the total long-chain moieties in brain EGP. The fatty acid composition of blubber triacylglycerols differed from those of the lipid classes from other tissues in that it had a very low ratio of n−6 to n−3 PUFA (0.3) as a result of a lower content of 20∶4n−6.     

Rainbow smelt population responses to species invasions and change in environmental condition

Invasive species can cause major disruptions in native food webs, yet the impact of species introductions and whether they will become invasive appears to be context-dependent. Rainbow smelt and alewife co-exist as invasive species in the Laurentian Great Lakes and as native species on the Atlantic coast of North America, but in Lake Champlain rainbow smelt is the dominant native forage fish and alewife are invasive. Alewife became abundant by 2007, providing an opportunity to explore the dynamics of these two species in a system where only one is invasive. We used data from a 28-year forage fish survey to compare demographics of rainbow smelt populations in three basins of Lake Champlain with different volumes, nutrient levels, and predator abundances. Rainbow smelt catch-per-unit-effort (CPUE) remained constant in the large, deep Main Lake before and after alewife invaded, but decreased in the two smaller basins. Declines were primarily a result of increased age-0 and age-1 mortality. Predation by top piscivores, system productivity, and competition for resources alone could not explain the patterns in CPUE across the basins. The mechanisms that allow alewife and rainbow smelt to co-exist could be related to system volume and oxythermal habitat availability, and may explain why the two species do not negatively affect each other in the Great Lakes. Summer hypoxia in the smaller basins could force individuals into smaller habitat volumes with higher densities of competitors and cannibalistic adult rainbow smelt. Habitat availability may mediate the impact of invasive alewife on native rainbow smelt.