Cold Atmospheric Plasma Jet Treatment Improves Human Keratinocyte Migration and Wound Closure Capacity without Causing Cellular Oxidative Stress

Cold Atmospheric Plasma (CAP) is an emerging technology with great potential for biomedical applications such as sterilizing equipment and antitumor strategies. CAP has also been shown to improve skin wound healing in vivo, but the biological mechanisms involved are not well known. Our study assessed a possible effect of a direct helium jet CAP treatment on keratinocytes, in both the immortalized N/TERT-1 human cell line and primary keratinocytes obtained from human skin samples. The cells were covered with 200 µL of phosphate buffered saline and exposed to the helium plasma jet for 10–120 s. In our experimental conditions, micromolar concentrations of hydrogen peroxide, nitrite and nitrate were produced. We showed that long-time CAP treatments (≥60 s) were cytotoxic, reduced keratinocyte migration, upregulated the expression of heat shock protein 27 (HSP27) and induced oxidative cell stress. In contrast, short-term CAP treatments (<60 s) were not cytotoxic, did not affect keratinocyte proliferation and differentiation, and did not induce any changes in mitochondria, but they did accelerate wound closure in vitro by improving keratinocyte migration. In conclusion, these results suggest that helium-based CAP treatments improve wound healing by stimulating keratinocyte migration. The study confirms that CAP could be a novel therapeutic method to treat recalcitrant wounds.     

Trends of night-time hourly cloud-cover values over Manila Observatory: ground-based remote-sensing observations using a digital camera for 13 months

ABSTRACT

Night-time cloud detection provides data sets of cloud-cover percentage. Although night-time cloud-cover data sets from satellite-based instruments are common, these data sets do not have relatively high temporal resolution. To quantify local temporal cloud-cover variability and to attain long-term cloud-cover measurements, ground-based instruments would be the appropriate apparatus. In this study, a digital camera is used to continuously gather images of the night sky at 5-min intervals over Manila Observatory (14.64° N, 121.07° E). For the first time in Manila, ground-based remote-sensing data gathered from October 2015 to October 2016 are analysed for hourly cloud cover. The results indicate that wet season has relatively higher cloud-cover values (median >40%) as compared to the dry season (median <40%). Moreover, cloud-cover values are observed to decrease during the night. For the wet season, August having the highest cloud-cover values has the highest value of change of hourly cloud-cover percentage (?0.82% h^?1). For the dry season, February having the lowest cloud-cover values has the highest value of change of hourly cloud-cover percentage (?1.04% h^?1).     

Effects of Dielectric Barrier Ambient Air Plasma on Two Brassicaceae Seeds: Arabidopsis thaliana and Camelina sativa

We investigated low-temperature plasma effects on two Brassicaceae seeds (A. thaliana and C. sativa) using dielectric barrier discharge in air. Comparisons of plasma treatments on seeds showed distinct responses on germination rate and speed. Optimal treatment time giving optimal germination is 15 min for A. thaliana with 85% increase compared to control after 48 h of germination and 1 min for C. sativa with 75% increase compared to control after 32 h of germination. Such germination increases are associated with morphological changes shown by SEM of seed surface. For better understanding at the biochemical level, seed surfaces were analyzed using gas chromatography-mass spectrometry which underlined changes of lipidic composition. For both treated seeds, there is a decrease of saturated (palmitic and stearic) fatty acids while treated C. sativa showed a decrease of unsaturated (oleic and linoleic) acids and treated A. thaliana an increase of unsaturated ones. Such lipid changes, specifically a decrease of hydrophobic saturated fatty acids, are coherent with the other analyses (SEM, water uptake and contact angle). Moreover, an increase in A. thaliana of unsaturated acids (very reactive) probably neutralizes plasma RONS effects thus needing longer plasma exposure time (15 min) to reach optimal germination. For C. sativa, 1 min is enough because unsaturated linoleic acid becomes lower in treated C. sativa (1.2 × 10⁷) compared to treated A. thaliana (3.7 × 10⁷).     

High-efficiency aerosol scatterometer that uses an integrating sphere for the calibration of multiwavelength lidar data

For the purpose of calibrating multiwavelength lidar data, we developed a scatterometer to measure the aerosol scattering coefficient at the ground level. The system is based on an integrating sphere, cw lasers (532 and 633 nm), and a controlled flow of the ambient air, including aerosol particles. The simulation study and experimental results indicate that the detection efficiency of this instrument is approximately 10%-40% better than that of an integrating nephelometer, because of the wider acceptance angle of the scattered light. The scattering coefficients measured at the two wavelengths, as well as the resulting value of the angstrom exponent, show good correlation with the results simultaneously measured with an integrating nephelometer and an optical particle counter.