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1.
K Sugahara Y Tanaka S Yamada N Seno H Kitagawa SM Haslam HR Morris A Dell 《Canadian Metallurgical Quarterly》1996,271(43):26745-26754
We prepared a series of oligosaccharides from king crab cartilage chondroitin sulfate K after exhaustive digestion with testicular hyaluronidase, and determined the structures of four tetrasaccharides and a pentasaccharide by fast atom bombardment mass spectrometry, high performance liquid chromatography analysis of chondroitinase AC-II digests, and 500-MHz 1H NMR spectroscopy. The tetrasaccharides shared the common core structure GlcAbeta1-3GalNAcbeta1-4GlcAbeta1-3GalNAc with various sulfation profiles. One structure was GlcAbeta1-3GalNAc(4S)beta1-4GlcAbeta1-3GalNAc(4S), whereas three of them have the following hitherto unreported structures including a novel glucuronate 3-O-sulfate: GlcA(3S)beta1-3GalNAc(4S)beta1-4GlcAbeta1-3GalNAc(4S), GlcAbeta1-3GalNAc(4S)beta1-4GlcA(3S)beta1-3GalNAc(4S), and GlcA(3S)beta1-3GalNAc(4S)beta1-4GlcA(3S)beta1-3GalNAc(4S), where 3S or 4S represents 3-O- or 4-O-sulfate, respectively. The structure of the pentasaccharide was determined as GlcA(3S)beta1-3GalNAc(4S)beta1-4GlcA(3S)beta1- 3GalNAc(4S)beta1-4GlcA. Chondroitinase ABC digestion of the tetrasaccharides with GlcA(3S) at the internal position destroyed the disaccharide unit containing GlcA(3S) derived from the reducing side and resulted in only the disaccharide unit from the non-reducing side. In contrast, these tetrasaccharides remained totally resistant to chondroitinase AC-II. The results indicated that it is necessary to reevaluate the disaccharide composition of chondroitin sulfate poly- or oligosaccharides purified from various biological sources, since they were usually determined after chondroitinase ABC digestion. It is probable that the structures containing GlcA(3S) would not have been detected. 相似文献
2.
Hiroko Ishii Maram H. Zahra Atushi Takayanagi Masaharu Seno 《International journal of molecular sciences》2021,22(4)
Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC50 at approximately 110 nM. The artificially humanized antibody is proposed to be a good candidate to target cancer cells overexpressing Cripto-1. 相似文献
3.
Summary A weak logic of programs is a formal system in which statements that mean the program halts cannot be expressed. In order to prove termination, we would usually have to use a stronger logical system. In this paper we show how we can prove termination of both iterative and recursive programs within a weak logic by augmenting the programs with counters and adding bound assertions on the counters as loop invariants and entry conditions. Thus, most of the existing verifiers which are based on a weak logic of programs can be used to prove termination of programs without any modification. We give examples of proofs of termination and of upper bounds on computation time that were obtained using a Pascal program verifier. The use of the method to locate causes of non-termination is also illustrated.This research was supported inpart by the Advanced Research Agency of the Office of the Secretary of Defence under contract DAHC 15-73-C-0435 相似文献
4.
Business process integration as a solution to the implementation of supply chain management systems 总被引:1,自引:0,他引:1
In the domain of supply chain management (SCM), various software packages have been developed for planning business strategies. To solve the problem of system productivity in applying planning packages, we propose a solution concept, business process integration (BPI), which fuses workflow and enterprise application integration (EAI) technology. Two characteristic policies are included in BPI. The first is to design the minimum set of business processes for real-time information sharing with planning packages without changing other processes. The second is to integrate several systems with EAI technology and to manage their execution with a workflow tool. Based on these policies, we propose various design templates and integration adapters. Our evaluation shows that using BPI, a target system can be developed with less manpower, in less time, and with higher quality than previous methods. 相似文献
5.
Norihisa Saeki Hiromi Sakamoto Teruhiko Yoshida 《International journal of molecular sciences》2014,15(5):7958-7973
Gastric cancer (GC) is one of the major malignant diseases worldwide, especially in Asia. It is classified into intestinal and diffuse types. While the intestinal-type GC (IGC) is almost certainly caused by Helicobacter pylori (HP) infection, its role in the diffuse-type GC (DGC) appears limited. Recently, genome-wide association studies (GWAS) on Japanese and Chinese populations identified chromosome 1q22 as a GC susceptibility locus which harbors mucin 1 gene (MUC1) encoding a cell membrane-bound mucin protein. MUC1 has been known as an oncogene with an anti-apoptotic function in cancer cells; however, in normal gastric mucosa, it is anticipated that the mucin 1 protein has a role in protecting gastric epithelial cells from a variety of external insults which cause inflammation and carcinogenesis. HP infection is the most definite insult leading to GC, and a protective function of mucin 1 protein has been suggested by studies on Muc1 knocked-out mice. 相似文献
6.
A simple screening procedure for heterotrophic nitrifying bacteria with oxygen-tolerant denitrification activity 总被引:4,自引:0,他引:4
Matsuzaka E Nomura N Nakajima-Kambe T Okada N Nakahara T 《Journal of Bioscience and Bioengineering》2003,95(4):409-411
Various naturally occurring strains of heterotrophic nitrifying bacteria were isolated by enrichment culture using acetamide as the C and N source, and 21 strains were identified as heterotrophic nitrifiers. Using a new simple procedure, these 21 strains were also investigated for the ability to carry out denitrifcation in the presence of oxygen. Several of the nitrifying strains were found to exhibit a distinct activity that allows for denitrifcation via nitrite (NO2-) in the presence of oxygen, indicating that they have an oxygen-tolerant denitrifcation system. A wide variety of bacteria possessing both nitrification and denitrifcation capabilities in the presence of oxygen were isolated and partially characterized by using the simple screening combinatorial procedure described in this paper. 相似文献
7.
Kanami Ikegami Tomoya Kawashima Shinji Hayashi Norihisa Moriya 《Journal of the Society for Information Display》2007,15(3):193-197
Abstract— A polymerizable liquid crystal (PLC), the orientation of which can be frozen, is useful for making retardation layers. In this paper, a new color filter (CF) with retardation‐controlling layers made of PLC is reported. It has a positive A‐plate and a negative C‐plate, both directly stacked on a color‐filter layer. These two retardation layers exhibit good orientation ability, and function well as retarders, even when they are only 1/10 or less as thick as ordinary retardation films. The new CF also has excellent thermal stability. The change in retardation after heat treatment at 200°C for 30 min is around 5%, and there is no observable peeling. A prototype VA‐LCD made with our new CF provides good optical compensation, with the light leakage being extremely low in all azimuthal directions. This technology is very useful for making thin, highly reliable color filters for LCDs, even with other modes. 相似文献
8.
Ranimol Stephen A. M. Siddique Fouran Singh Lekshmi Kailas Seno Jose Kuruvilla Joseph Sabu Thomas 《应用聚合物科学杂志》2007,105(2):341-351
The effect of microfillers on the thermal stability of natural rubber (NR), carboxylated styrene butadiene rubber (XSBR) latices, and their 70/30 NR/XSBR blend were studied using thermogravimetric method. Microcomposites of XSBR and their blend were found to be thermally more stable than unfilled samples. The activation energy needed for the degradation of polymer chain was calculated from Coats‐Redfern plot. Activation energy needed for the thermal degradation of filled samples was higher than unfilled system. It indicated the improved thermal stability of the filled samples. The ageing resistance of the micro‐filled samples was evaluated from the mechanical properties of aged samples. The thermal ageing was carried out by keeping the samples in hot air oven for 7 days at 70°C. The mechanical properties such as tensile strength, modulus at 300% elongation, and strain at break were computed. As compared to unfilled samples, micron‐sized fillers reinforced systems exhibited higher ageing resistance. Finally, an investigation was made on the influence of ion‐beam irradiation on microcomposites of NR, XSBR latices, and their 70/30 blend systems using 28Si8+ performed at 100 MeV. The surface changes of the samples after irradiation were analyzed using X‐ray photoelectron spectroscopy. The results of XPS measurements revealed that the host elements were redistributed without any change in binding energies of C1s, O1s, and Si2p. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007 相似文献
9.
Nakai M. Akui S. Seno K. Meguro T. Seki T. Kondo T. Hashiguchi A. Kawahara H. Kumano K. Shimura M. 《Solid-State Circuits, IEEE Journal of》2005,40(1):28-35
High-performance and low-power microprocessors are key to PDA applications. A dynamic voltage and frequency management (DVFM) scheme with leakage power compensation effect is introduced in a microprocessor with 128-bit wideband 64-Mb embedded DRAM. The DVFM scheme autonomously controls clock frequency from 8 to 123 MHz in steps of 0.5 MHz and also adaptively controls supply voltage from 0.9 to 1.6 V in steps of 5 mV, achieving 82% power reduction in personal information management scheduler application and 40% power reduction in MPEG4 movie playback. This low-power embedded microprocessor, fabricated with 0.18-/spl mu/m CMOS embedded DRAM technology, enables high-performance operations such as audio and video applications. As process technology shrinks, this adaptive leakage power compensation scheme will become more important in realizing high-performance and low-power mobile consumer applications. 相似文献
10.
Yoshinori Nishida Norihisa Izawa Yukio Kuramasu 《Metallurgical and Materials Transactions A》1999,30(3):839-844
Separation of matrix metals in composites was tried on alumina short fiber-reinforced aluminum and 6061 alloy composites and
SiC whisker-reinforced 6061 alloy composite for recycling. It is possible to separate molten matrix metals from fibers in
the composites using fluxes that are used for melt treatment to remove inclusions. About 50 vol pct of the matrix metals was
separated from the alumina short fiber-reinforced composites. The separation ratio of the matrix from the SiC whisker-reinforced
6061 alloy composite was low and about 20 vol pct. The separation mechanism was discussed thermodynamically using interface
free energies. Since the flux/fiber interface energy is smaller than the aluminum/fiber interface energy, the replacement
of aluminum with fluxes in composites takes place easily. Gases released by the decomposition of fluxes act an important role
in pushing out the molten matrix metal from the composite. The role was confirmed by the great amount cavity formed in the
composite after the matrix metal flowed out. 相似文献