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SecA is a dynamic protein that undergoes ATP-dependent membrane cycling to drive protein translocation across the Escherichia coli inner membrane. To understand more about this process, azide-resistant (azi) and signal sequence suppressor (prlD) alleles of secA were studied. We found that azide resistance is cold sensitive because of a direct effect on protein export, suggesting that SecA-membrane interaction is regulated by an endothermic step that is azide inhibitable. secG function is required for expression of azide-resistant and signal sequence suppressor activities of azi and prlD alleles, and in turn, these alleles suppress cold-sensitive and export-defective phenotypes of a secG null mutant. These remarkable genetic observations support biochemical data indicating that SecG promotes SecA membrane cycling and that this process is dependent on an endothermic change in SecA conformation. 相似文献
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Sustaining Rural Landscapes: the role of integrated tourism 总被引:2,自引:0,他引:2
The aim of a European Union-funded research project entitled 'Supporting and Promoting Integrated Tourism in Europe's Lagging Rural Regions' (SPRITE) is to analyse and develop the potential for better-integrated tourism in the rural landscapes of Europe. Integrated tourism can be defined as that which is explicitly linked to the localities in which it takes place and, in practical terms, has clear connections with local resources, activities, products, production and service industries, and a participatory local community. Enhancing the sustainable economic potential of rural landscapes requires partnerships among rural people and the sustainable yet productive use of rural resources. A 'culture economy' approach to integrated tourism is proposed, which emphasizes the importance of local identity, the strategic commodification of resources and of place, and the importance of extra-local forces in enabling local activities. It is suggested that this approach may represent a shift beyond more traditional approaches to rural development. 相似文献
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Pharmacological interactions are important when nuclear medical procedures are applied to patients under drug therapy, or drug provocation. This study compares in baboon models (regional) cerebral blood flow [(r)CBF] results from 99mTc-HMPAO and 123I-iodoamphetamine [123I(IMP)] each with and without acetazolamide, the latter a suggested drug for testing cerebrovascular reserve. Expected differences in cerebral uptake were observed between the two radio-tracers without acetazolamide. The increase in tracer uptake resulting from acetazolamide is significantly enhanced for 123I(IMP), which could have diagnostic implications. 相似文献
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Finite element analyses of repaired articular surfaces. 总被引:1,自引:0,他引:1
J S Wayne S L Woo M K Kwan 《Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine》1991,205(3):155-162
The response to a compressive load of a repaired cartilage surface, consisting of full-thickness repair tissue adjacent to normal cartilage, was predicted by the u-p finite element method and compared to that of a normal cartilage surface under the same loading conditions. By individually varying the aggregate modulus, permeability and Poisson's ratio for the repair tissue, analyses were performed to assess the contributions of each to the changes in mechanical behaviour. In comparing the repaired to normal surfaces, the presence of a softer repair tissue resulted in increased axial and decreased radial deformations at any given time point, while a repaired surface with an increased permeability compressed more easily due to the increased fluid flow and caused equilibrium to occur sooner. For smaller Poisson's ratio, the axial deformation was not different from normal if the aggregate modulus was the same as normal; however, the radial expansion was reduced as the repair tissue experienced a larger volume change. These results indicate that the presence of repair tissue in a joint surface can have a strong influence on the mechanical behaviour of the surface. 相似文献
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An adaptive analog continuous-time biquadratic filter is realized in a 2-μm digital CMOS process for operation at 300 kHz. The biquad implements the notch, bandpass and low-pass transfer functions. The only parameter adapted is the resonant frequency of the biquad, which is identical to the notch frequency and the bandpass center frequency. The update method is based on a least-means-square algorithm which adapts the notch frequency to minimize the power at the notch filter output. The actual update is modified to reduce the circuit complexity to one biquad and one correlator. When the filter is tracking a sinusoid, this update generates a ripple-free gradient that decreases tracking error. Applications include phase-frequency detectors, FM demodulators (linear and frequency shift keying), clock extractors, and frequency acquisition aids for phase-locked loops and Costas loops. Measured results from experimental prototypes are presented. Nonidealities of an all-analog implementation are discussed, along with suggestions to improve performance 相似文献
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Personality is an essential feature for creating socially interactive robots. Studies on this dimension will facilitate enhanced human–robot interaction (HRI). Using AIBO, a social robotic pet developed by Sony, we examined the issue of personality in HRI. In this gender-balanced 2 (AIBO personality: introvert vs. extrovert) by 2 (participant personality: introvert vs. extrovert) between-subject experiment (N = 48), we found that participants could accurately recognize a robot's personality based on its verbal and nonverbal behaviors. In addition, various complementarity attraction effects were found in HRI. Participants enjoyed interacting with a robot more when the robot's personality was complementary to their own personalities than when the robot's personality was similar to their own personalities. The same complementarity attraction effect was found in participants' evaluation of the robot's intelligence and social attraction. Participants' feelings of social presence during the interaction were a significant mediator for the complementarity attraction effects observed. Practical and theoretical implications of the current study for the design of social robots and the study of HRI were discussed. 相似文献