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1.
Quantitative UPLC‐MS/MS analysis of chlorogenic acid derivatives in antioxidant fractionates from dandelion (Taraxacum officinale) root 下载免费PDF全文
Owen Kenny Thomas J. Smyth Chandralal M. Hewage Nigel P. Brunton 《International Journal of Food Science & Technology》2015,50(3):766-773
While qualitative studies have identified chlorogenic acids in antioxidant extracts, particularly ethyl acetate‐derived extracts, of Taraxacum officinale, quantitative analysis of these phenolic compounds remains largely unreported for this species. In this study, bioactivity‐guided fractionation of an antioxidant crude ethyl acetate extract (DPPH = 295.481 ± 0.955 mg TE g?1 extract) from T. officinale root resulted in a number of reverse‐phase fractions that demonstrated high antioxidant activity (DPPH = 1058.733–1312.136 mg TE g?1 extract), stronger than that of the synthetic antioxidant Trolox®. UPLC‐MS/MS screening of these fractions for the presence of selected mono‐ and di‐caffeoylquinic acids revealed large quantities of 1,5‐dicaffeoylquinic acid present in several fractions (853.052–907.324 μg mg?1), respectively. Due to the antioxidant potency and high levels of 1,5‐dicaffeoylquinic acid observed in these fractions, it was concluded that specifically this chlorogenic acid derivative is a major contributor to the antioxidant efficacy of dandelion root. 相似文献
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Cocaine lowers brain reward thresholds, reflecting increased brain reward function. The authors investigated whether, similar to acute cocaine administration, cocaine-predictive conditioned stimuli would lower intracranial self-stimulation (ICSS) thresholds. Rats received a saline injection for 5 days, a cocaine injection (10 mg/kg) for 20 consecutive days, then saline for 5 additional days. Thresholds were measured immediately before and 10 min after each injection. The initial 5 saline injections had no effect on thresholds, whereas cocaine significantly lowered thresholds for 20 days. There was no tolerance or sensitization to this effect of cocaine over days. During the last 5 days when cocaine administration was substituted with saline, rats demonstrated a conditioned lowering of thresholds during the 2nd daily ICSS session. These data demonstrate that cocaine-predictive conditioned stimuli induce a conditioned facilitation of brain reward function. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Maria E. Amato Antonio Grassi Kenny B. Lipkowitz Giuseppe M. Lombardo Giuseppe C. Pappalardo Claudia Sadun 《Journal of Inorganic and Organometallic Polymers and Materials》1996,6(3):237-253
Suitable parameter sets for the CHARMm force field were derived for the structural units in polychlorophosphazene [P=N, P N, P Cl] using the Dinur Hagler energy second derivative procedure based on quantum mechanical SCI calculations using the 6–31G* basis set. To validate the reliability of the parameter set, structural results obtained with CHARMm for the adopted model compounds (OP2NCl5 and OP3N2Cl5) were compared with those derived fromab initio quantum mechanics using the 6–31G* basis set. Application of molecular dynamics (MD) simulations in combinatioin with the available X-ray diffraction data provided structural and conformational information on the polymer. The calculation made using the periodic boundary conditions (PBC) agree well with the polychlorophosphazene ordered in a monoclinic unit cell (a=5.98,b=12.99,c=4.92 A; β=111.7). This model was stabilized mainly by the image atoms contribution to the electrostatic energy term and had aquasi-planar conformation of the backbone chain (glide symmetry). The MD calculations also provided evidence that the difference between single and double PN bonds is less marked than that measured experimentally. This result is, however, in agreement with more recent and accurate X-ray studies on poly(methylphosphazene). Validation of the polymer model provided a complete picture, otherwise experimentally inaccessible, of the internal fluctuations of the polymeric chains. 相似文献
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H Rodgers M Sudlow R Dobson RA Kenny RG Thomson 《Canadian Metallurgical Quarterly》1997,47(418):309-310
The demand for anticoagulation services is rising. Warfarin anticoagulation has been shown to reduce the risk of stroke in patients with non-valvular atrial fibrillation by 68%. This raises issues about how services are best organized to initiate and monitor anticoagulation in this potentially large group of patients. We report the results of a regional postal survey undertaken to describe the views of general practitioners and consultants regarding warfarin anticoagulation in light of this potentially high increase in demand. 相似文献
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Kenny P. Ouellet P. Dehak N. Gupta V. Dumouchel P. 《IEEE transactions on audio, speech, and language processing》2008,16(5):980-988
We propose a new approach to the problem of estimating the hyperparameters which define the interspeaker variability model in joint factor analysis. We tested the proposed estimation technique on the NIST 2006 speaker recognition evaluation data and obtained 10%-15% reductions in error rates on the core condition and the extended data condition (as measured both by equal error rates and the NIST detection cost function). We show that when a large joint factor analysis model is trained in this way and tested on the core condition, the extended data condition and the cross-channel condition, it is capable of performing at least as well as fusions of multiple systems of other types. (The comparisons are based on the best results on these tasks that have been reported in the literature.) In the case of the cross-channel condition, a factor analysis model with 300 speaker factors and 200 channel factors can achieve equal error rates of less than 3.0%. This is a substantial improvement over the best results that have previously been reported on this task. 相似文献
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Enteropathogenic Escherichia coli (EPEC) secretes at least five proteins. Two of these proteins, EspA and EspB (previously called EaeB), activate signal transduction pathways in host epithelial cells. While the role of the other three proteins (39, 40, and 110 kDa) remains undetermined, secretion of all five proteins is under the control of perA, a known positive regulator of several EPEC virulence factors. On the basis of amino-terminal protein sequence data, we cloned and sequenced the gene which encodes the 110-kDa secreted protein and examined its possible role in EPEC signaling and interaction with epithelial cells. In accordance with the terminology used for espA and espB, we called this gene espC, for EPEC-secreted protein C. We found significant homology between the predicted EspC protein sequence and a family of immunoglobulin A (IgA) protease-like proteins which are widespread among pathogenic bacteria. Members of this protein family are found in avian pathogenic Escherichia coli (Tsh), Haemophilus influenzae (Hap), and Shigella flexneri (SepA). Although these proteins and EspC do not encode IgA protease activity, they have considerable homology with IgA protease from Neisseria gonorrhoeae and H. influenzae and appear to use a export system for secretion. We found that genes homologous to espC also exist in other pathogenic bacteria which cause attaching and effacing lesions, including Hafnia alvei biotype 19982, Citrobacter freundii biotype 4280, and rabbit diarrheagenic E. coli (RDEC-1). Although these strains secrete various proteins similar in molecular size to the proteins secreted by EPEC, we did not detect secretion of a 110-kDa protein by these strains. To examine the possible role of EspC in EPEC interactions with epithelial cells, we constructed a deletion mutant in espC by allelic exchange and characterized the mutant by standard tissue culture assays. We found that EspC is not necessary for mediating EPEC-induced signal transduction in HeLa epithelial cells and does not play a role in adherence or invasion of tissue culture cells. 相似文献
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Martin Sulzmann Kenny Zhuo Ming Lu 《Electronic Notes in Theoretical Computer Science》2006,148(2):239
We consider the problem of integrating XDuce into ML. This is difficult because of incompatible type and value representations. Our solution is a type-driven translation scheme from XDuce to ML based on a structured representation of XDuce values. XDuce type inference guides the insertion of appropriate coercion functions to translate regular expression pattern matching and uses of semantic subtyping. We can extend our translation scheme to include ML function calls and patterns into XDuce. Thus, we can embed XDuce into ML. Our results allow to enrich the ML language with support for dealing with semi-structured data. 相似文献
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