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排序方式: 共有110条查询结果,搜索用时 15 毫秒
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Antonio Sánchez‐Díaz Marta Izquierdo Salvatore Filippone Nazario Martin Emilio Palomares 《Advanced functional materials》2010,20(16):2695-2700
Organic solar cells made using a blend of DPM12 and P3HT are studied. The results show that higher Voc can be obtained when using DPM12 in comparison to the usual mono‐substituted PCBM electron acceptor. Moreover, better device performances are also registered when the cells are irradiated with sun‐simulated light of 10–50 mW cm?2 intensity. Electrochemical and time‐resolved spectroscopic measurements are compared for both devices and a 100‐mV shift in the density of states (DOS) is observed for DPM12/P3HT devices with respect to PCBM/P3HT solar cells and slow polaron‐recombination dynamics are found for the DPM12/P3HT devices. These observations can be directly correlated with the observed increase in Voc, which is in contrast with previous results that correlated the higher Voc with different ideality factors obtained using dark‐diode measurements. The origin for the shift in the DOS can be correlated to the crystallinity of the blend that is influenced by the properties of the included fullerene. 相似文献
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Patricia Garrido-Pascual Ana Alonso-Varona Begoa Castro María Burn Teodoro Palomares 《International journal of molecular sciences》2020,21(24)
Oxidative stress associated with neuroinflammation is a key process involved in the pathophysiology of neurodegenerative diseases, and therefore, has been proposed as a crucial target for new therapies. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for neuroprotection. These cells can be preconditioned by exposing them to mild stress in order to improve their response to oxidative stress. In this study, we evaluate the therapeutic potential of hASCs preconditioned with low doses of H2O2 (called HC016 cells) to overcome the deleterious effect of oxidative stress in an in vitro model of oligodendrocyte-like cells (HOGd), through two strategies: i, the culture of oxidized HOGd with HC016 cell-conditioned medium (CM), and ii, the indirect co-culture of oxidized HOGd with HC016 cells, which had or had not been exposed to oxidative stress. The results demonstrated that both strategies had reparative effects, oxidized HC016 cell co-culture being the one associated with the greatest recovery of the damaged HOGd, increasing their viability, reducing their intracellular reactive oxygen species levels and promoting their antioxidant capacity. Taken together, these findings support the view that HC016 cells, given their reparative capacity, might be considered an important breakthrough in cell-based therapies. 相似文献
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Current advances in the non‐chromatographic fractionation and characterization of PEGylated proteins
Oscar Aguilar Jorge Benavides Marco Rito‐Palomares 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2011,86(1):18-25
For more than 30 years, PEGylation has been used to improve the physicochemical properties of several proteins and therapeutic drugs having a major impact in the biopharmaceutical industry. The purification of PEGylated proteins usually involves two basic challenges: (1) the separation of PEG‐proteins from other reaction products; and (2) the sub‐fractionation of PEG‐proteins on the basis of their degree of PEGylation and positional isomerism. Currently, most PEGylated protein purification processes are based on chromatographic techniques, especially size exclusion chromatography (SEC) and ion exchange chromatography (IEX). Nonetheless, other less frequently used strategies based on non‐chromatographic techniques such as ultrafiltration, electrophoresis, capillary electrophoresis, and aqueous two‐phase systems have been developed in order to fractionate and analyze PEGylated derivates. This review presents current advances in some of the most widely used non‐chromatographic strategies for the fractionation and analysis of PEG‐protein conjugates. Copyright © 2010 Society of Chemical Industry 相似文献
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K. Mayolo‐Deloisa C. Machín‐Ramírez M. Rito‐Palomares M. R. Trejo‐Hernández 《化学工程与技术》2011,34(8):1368-1372
Laccase partially purified from residual compost of Agaricus bisporus by an aqueous two‐phase system (Lac ATPS) was used in degrading polycyclic aromatic hydrocarbons: fluorene (Flu), phenanthrene (Phe), anthracene (Ant), benzo[a]pyrene (BaP), and benzo[a]anthracene (BaA). The capacity of the enzyme to oxidize polyaromatic compounds was compared to that of the crude laccase extract (CE). After treatment of 72 h, Lac ATPS and CE were not capable of oxidizing Flu and Phe, while Ant, BaP, and BaA were oxidized, resulting in percentages of oxidation of 11.2 ± 1, 26 ± 2, and 11.7 ± 4 % with CE, respectively. When Lac ATPS was used, the following percentages of oxidation were obtained: 11.4 ± 3 % for Ant, 34 ± 0.1 % for BaP, and 13.6 ± 2 % for BaA. The results reported here demonstrate the potential application of Lac ATPS for the oxidation of polycyclic aromatic hydrocarbons. 相似文献
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Francisco J. Rodriguez-Lozano Juan C. Gámez-Granados Jose M. Palomares Joaquín Olivares 《Computer-Aided Civil and Infrastructure Engineering》2023,38(16):2339-2354
Automatic crack classification plays an essential role in road maintenance. Using many features for the classification is inefficient for implementing embedded systems with low computational resources makes it difficult. Therefore, this work proposes a new data dimensionality reduction (DDR) for crack classification algorithms (DDR4CC). DDR4CC reduces the required information about the cracks to only four features. Using these features, the images can be classified into longitudinal, transverse, and alligator cracks or healthy pavement. DDR4CC is compared with eight DDR methods, and the reduced set of features is analyzed using five different classification algorithms. Besides, five different datasets, generated by a combination of several public datasets, are used. We are proposing a simple DDR method with high interpretability of the data, obtaining very fast computation and high accuracy. Experiments show that DDR4CC enhances the results of the classification algorithms, providing almost perfect classifiers with a minimum computation time. 相似文献
10.
Aqueous two‐phase systems for the recovery of a recombinant viral coat protein from Escherichia coli
Marco Rito‐Palomares Anton P
J Middelberg 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2002,77(9):1025-1029
In this study the use of an aqueous two‐phase system (ATPS) following the direct chemical extraction of a recombinant viral coat protein, from the cytoplasm of Escherichia coli, is evaluated. The driving force is the need to establish an economically‐viable process for the manufacture of a vaccine against human papilloma infection. The partition behaviour of recombinant L1 protein, the major structural protein of the virus, and DNA was investigated in a polyethylene glycol (PEG)–phosphate system. An evaluation of system parameters including PEG molecular mass and the concentrations of PEG and phosphate was conducted, to estimate conditions under which the L1 protein and DNA partition to opposite phases. ATPS extraction comprising a volume ratio of 1.00, PEG 1000 (18.0%(w/w)) and phosphate (15.0%(w/w)) provided the conditions for accumulation of DNA into the bottom phase and concentration of L1 protein into the opposite phase (ie partition coefficient of DNA; ln KDNA < 0.0 and partition coefficient of L1; ln KL1 > 2.5). The findings reported here demonstrate the potential of ATPS to recover recombinant protein released from E coli by direct chemical extraction. © 2002 Society of Chemical Industry 相似文献