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排序方式: 共有1718条查询结果,搜索用时 15 毫秒
1.
M Sternfeld G Ming H Song K Sela R Timberg M Poo H Soreq 《Canadian Metallurgical Quarterly》1998,18(4):1240-1249
Accumulated indirect evidence suggests nerve growth-promoting activities for acetylcholinesterase (AChE). To determine unequivocally whether such activities exist, whether they are related to the capacities of this enzyme to hydrolyze acetylcholine and enhance synapse development, and whether they are associated with alternative splicing variants of AChEmRNA, we used four recombinant human AChEDNA vectors. When Xenopus laevis embryos were injected with a vector expressing the synapse-characteristic human AChE-E6, which contains the exon 6-encoded C terminus, cultured spinal neurons expressing this enzyme grew threefold faster than co-cultured control neurons. Similar enhancement occurred in neurons expressing an insertion-inactivated human AChE-E6-IN protein, containing the same C terminus, and displaying indistinguishable immunochemical and electrophoretic migration properties from AChE-E6, but incapable of hydrolyzing acetylcholine. In contrast, the nonsynaptic secretory human AChE-I4, which contains the pseudointron 4-derived C terminus, did not affect neurite growth. Moreover, no growth promotion occurred in neurons expressing the catalytically active C-terminally truncated human AChE-E4, demonstrating a dominant role for the E6-derived C terminus in neurite extension. Also, AChE-E6 was the only active enzyme variant to be associated with Xenopus membranes. However, postsynaptic length measurements demonstrated that both AChE-E6 and AChE-E4 enhanced the development of neuromuscular junctions in vivo, unlike the catalytically inert AChE-E6-IN and the nonsynaptic AChE-I4. These findings demonstrate an evolutionarily conserved synaptogenic activity for AChE that depends on its hydrolytic capacity but not on its membrane association. Moreover, this synaptogenic effect differs from the growth-promoting activity of AChE, which is unrelated to its hydrolytic capacity yet depends on its exon 6-mediated membrane association. 相似文献
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In this paper, mobility parameters for enhancement-mode N-channel 4H SiC MOSFETs are extracted and implemented into 2-D device simulation program and SPICE circuit simulator. The experimental data were obtained from lateral N-channel 4H SiC MOSFETs with nitrided oxide–semiconductor interfaces, exhibiting normal mobility behavior. The presence of increasing interface-trap density (Dit) toward the edge of the conduction band is included during the 2-D device simulation. Using measured distribution of interface-trap density for simulation of the transfer characteristics leads to a good agreement with the experimental transfer characteristic. 相似文献
4.
Susan J. Masten Kuan‐Chung Chen Jaime Graulau Subhash L. Kari Kyung‐Hyuk Lee 《工程教育杂志》2002,91(1):69-80
The use of computer‐based technology is becoming more prevalent in the classroom. As a part of an educational research project sponsored by the GE Foundation, strategies for augmenting a course, Introduction to Environmental Engineering (CE 280), were investigated including cross‐disciplinary experiences in teamwork, design, and the use of advanced teaching technologies such as the web. Interactive tools to assist student learning were developed and refined. Efforts have focused on developing an extensive website, web‐based quizzes and homework assignments, and tutorials. Base groups were used to provide both intellectual and emotional support to students. This paper summarizes the development of this course and the impact of rapid feedback on the progression of student understanding. 相似文献
5.
Organic‐acid‐catalyzed sol–gel route for preparing poly(methyl methacrylate)–silica hybrid materials
Jui‐Ming Yeh Kuan‐Yeh Huang Chung‐Feng Dai B. G. Chand Chang‐Jian Weng 《应用聚合物科学杂志》2008,110(4):2108-2114
In this study, a series of organic–inorganic hybrid sol–gel materials consisting of a poly(methyl methacrylate) (PMMA) matrix and dispersed silica (SiO2) particles were successfully prepared through an organic‐acid‐catalyzed sol–gel route with N‐methyl‐2‐pyrrolidone as the mixing solvent. The as‐synthesized PMMA–SiO2 nanocomposites were subsequently characterized with Fourier transform infrared spectroscopy and transmission electron microscopy. The solid phase of organic camphor sulfonic acid was employed to catalyze the hydrolysis and condensation (i.e., sol–gel reactions) of tetraethyl orthosilicate in the PMMA matrix. The formation of the hybrid membranes was beneficial for the physical properties at low SiO2 loadings, especially for enhanced mechanical strength and gas barrier properties, in comparison with the neat PMMA. The effects of material composition on the thermal stability, thermal conductivity, mechanical strength, molecular permeability, optical clarity, and surface morphology of the as‐prepared hybrid PMMA–SiO2 nanocomposites in the form of membranes were investigated with thermogravimetric analysis, differential scanning calorimetry, dynamic mechanical analysis, gas permeability analysis, ultraviolet–visible transmission spectroscopy, and atomic force microscopy, respectively. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008 相似文献
6.
提出了一种基于6LoWPAN的物联网总体方案,同时对协议中的Mesh字段进行了改进,然后在网络模拟环境下对本协议平均传输成功率、平均延迟时间的性能进行了比较分析,并在仿真环境中进行了实验验证工作。实验数据表明,改进后的协议具有更好的性能。 相似文献
7.
利用测井资料评价储集层性质的探讨 总被引:2,自引:0,他引:2
根据对储集层之简化导电模型的理论分析,提出了一个由电阻率、孔隙度信息表示的、反映储层性质的“孔隙结构参数S”。实际应用表明,S参数在评价储集层性质、计算储层渗透率等方面具有一定的使用价值。 相似文献
8.
年最大洪峰流量预测,受较多的复杂因素的影响,不确定性较强,用常规统计方法做出准确预报具有较大困难。从水文序列本身出发,提出将投影回归模型应用于年最大洪峰流量预测,为了更好获得投影寻踪模型参数和预测精度,提出了运用延迟相关系数法确定回归预测因子、群居蜘蛛算法优化投影寻踪模型最佳投影方向参数a、利用最小二乘法确定多项式的权系数c、岭函数个数M的群居蜘蛛优化投影寻踪年最大洪峰流量预测模型,结合长江宜昌站(1882年-2004年)的年最大洪峰流量资料进行实例预测,训练阶段平均绝对相对误差为8.61%,预测阶段平均绝对相对误差为10.51%,该模型预测效果较好,模型结果稳定,可有效应用于年最大洪峰流量预测。 相似文献
9.
随着计算机技术及外围设备的提高和完善 ,计算机辅助设计和计算机辅助工程技术得到了日益广泛应用 ,绘图技术也得到了很大的发展 ,并已成为工程制图发展的必然趋势。我公司应用美国AUTODESK公司推出的新版AutoCAD2 0 0 2中文版本 ,是当前国际上最为先进和流行的绘图软件之一 ,具有以下增强功能。1 新增设计中心图库 ,在绘图过程中 ,可边绘图 ,边自动将所绘单元图储存在设计中心库中 ,这样可重复和快速使用相同或相近单元图 ,或在绘制新图时 ,可从中心库中随时提取相同或相近单元图形来绘制新图。其次 ,在绘图过程中 ,也可把… 相似文献
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