Immune response to baculovirus expressed protein fragment amino acids 190-289 of respiratory syncytial virus (RSV) fusion protein

At least two neutralizing epitopes have been identified in the amino acid (aa) sequence 190-289 of the RSV fusion protein. The authors expressed this region in insect cells (bF190-289) and compared the immune response to bF190-289 with that induced by baculovirus expressed full-length fusion protein (bF). As with bF, mice primed with bF190-289 produced exclusively antibodies of IgG1 isotype, generated neutralizing antibodies, reduced significantly the virus titer (about a half log10 reduction) after RSV challenge and induced a Helper T (Th) 2 cell response in mediastinal lymph node cells (MLNC) restimulated in vitro. Thus, the aa sequence 190-289 represents a major immunogenic region of the RSV fusion protein.     

A novel packaging approach for RF MEMS switching functions on alumina substrate

This paper reports the design of a hermetic-compatible wafer-scale package for RF MEMS based components. The presented packaging concept consists in encapsulating the whole RF device or subsystem instead of encapsulating each MEMS component separately, which will reduce the device size and cost. This approach is based on the MEMS fabrication technology on ceramic substrate and the use of laser drilled vias hole techniques to realize full metallized vias in alumina substrates. These vias holes will allow a low loss RF signal transmission inside the package without breaking its hermeticity. Hence, several packaged switching networks prototypes, based on ohmic contact MEMS switches, have been designed following this approach and the packaging electromagnetic impact on these components has been especially studied to result on good performance devices.     

Sewage impact on shellfish microbial contamination.

Coastal areas are frequently contaminated by microorganisms of human origin, due to high population density and low seawater renewal. To evaluate the impact of wastewater input on shellfish quality, a study was conducted in Brittany (France) over a period of 20 months. A hydrodynamic model was used to simulate wastewater impact on microbial water quality. To validate the model, wastewater from the three main sewage treatment plants and shellfish from three sites were sampled monthly. Bacterial indicators (E. coli), F-RNA phages were searched for by culture and noroviruses by RT-PCR and hybridisation. These microorganisms were detected in the three effluents and clams, with no marked seasonal variation. The microbial concentrations in the two oyster beds, distant from the effluent outfall, were low, and only three of the samples were positive for norovirus. For simulation, the winter wastewater inputs of E. coli and phages were calculated and an estimation for norovirus flux was made from the epidemic situation in the population. The microbial behaviour was included in the model by a decay-rate factor. Results from the model calculations were found to be very similar to E. coli and phage concentrations observed in shellfish. For noroviruses, the model indicated that shellfish distant from the wastewater input were under the detection limit of the RT-PCR method. This study demonstrated the use of modelisation to interpret norovirus contamination in various areas.     

Epitope mapping of the major inner capsid protein of group A rotavirus using peptide synthesis

Nucleic acid amplification by polymerase chain reaction (PCR) is a very powerful technique in terms of sensitivity but is limited in terms of ability to perform accurate quantitation. While there is a theoretical correlation between copies of input target sequence and those of PCR product, the quantitative nature of this relationship is obscured by unpredictable variations in reaction conditions and by inhibitory and/or stimulatory substances which might be present in sample preparations, especially those derived from biological fluids. To reliably estimate copies of input DNA target from PCR product, we designed a combination of internal and external control systems coupled to DNA/RNA hybridization and enzymatic immunodetection techniques. The internal control system served to monitor amplification efficiency and to correct for the effects of inhibitors or stimuli on the efficiency of the DNA amplification. The assay is quantitative, nonisotopic, and can be widely applied to assessment of the quantity of DNA present in a wide range of preparations.     

Adhesion of human pathogenic enteric viruses and surrogate viruses to inert and vegetal food surfaces

Enteric viruses, particularly human Noroviruses (NoV) and hepatitis A virus (HAV), are key food-borne pathogens. The attachment of these pathogens to foodstuff and food-contact surfaces is an important mechanism in the human contamination process. Studies were done to investigate the nature of the physicochemical forces, such as hydrophobic and electrostatic ones, involved in the interaction virus/matrix but, at this day, only few data are available concerning surface properties of viruses and prediction of the adhesion capacity of one specific virus onto matrices is still very difficult. The purpose of this study was to propose a reference system, including a representative virus surrogate, able to predict as close as possible behaviour of pathogenic viruses in term of adhesion on inert (stainless steel and polypropylene) and food surfaces (lettuce leaves, strawberries and raspberries). The adhesion of human pathogenic enteric viruses, cultivable strain of HAV and non-cultivable strains of human NoV (genogroups I and II), have been quantified and compared to these of human enteric viruses surrogates, included the MNV-1 and three F-specific RNA bacteriophages (MS2, GA and Qβ). A standardized approach was developed to assess and quantify viral adhesion on tested matrices after a contact time with each virus using real-time RT-PCR. Methods used for virus recovery were in accordance with the CEN recommendations, including a bovine Enterovirus type 1 as control to monitor the efficiency of the extraction process and amplification procedure from directly extracted or eluted samples. The adhesion of human pathogenic viruses, ranging from 0.1 to 2%, could be comparable for all matrices studied, except for NoV GII on soft fruits. Adhesion percentages obtained for the studied surrogate virus and phages were shown to be comparable to those of HAV and NoV on inert and lettuce surfaces. The MNV-1 appeared as the best candidate to simulate adhesion phenomena of all human pathogenic enteric viruses on all studied surfaces, while MS2 and GA bacteriophages could be a good alternative as model of viral adhesion on inert and lettuce surfaces. These results will be usable to design relevant experimental systems integrating adhesion behaviour of enteric viruses in the assessment of the efficiency of a technological or hygienic industrial process.     

Performance-based indicators for controlling geosynthetic clay liners in landfill applications

The results of a project aimed at identifying performance-based indicators that can be used by landfill operators to check the suitability of GCLs for bottom barrier applications are presented. The general methodology consisted of performing detailed characterization of the prevalent GCLs used in France for landfill barrier applications, before and after prolonged contact with several fluids during oedo-permeameter tests. Results of mineralogical analysis illustrate the variety of composition of the tested bentonites, which in addition to smectite clay contain a large number of accessory minerals. For one of the GCLs tested, the proportion of smectite was lower than 30 wt%, which highlights the limitations of the generic designation “bentonite” when referring to GCLs destined to landfill applications. Results also underline the correlation between cation exchange capacity (CEC) and smectite content, the correlation between free swell volume and proportion of exchangeable sodium and the influence of the bentonite's calcium carbonate fraction on hydraulic conductivity. Transmission electron microscopy (TEM) photographs illustrate the effect of cation exchange on clay microstructure, with the formation of clay particles which lead to increased hydraulic conductivity. The exchange is also documented by exchangeable cation analyses. Results of isotopic analyses indicate that information provided by suppliers with respect to the “natural” versus “activated” nature of the bentonite, may sometimes be arbitrary and related to factors that are very difficult to check in practice, even by the suppliers themselves. This further underlines the need for performance-based indicators, rather than generic designations, to provide objective information regarding GCL suitability for landfill applications. Several performance-based indicators are selected in order to provide practical tools for checking the suitability of sodium-bentonite GCLs in bottom barrier applications and limit values are proposed.     

An information processing model of some seriation tasks.

Children were video-tape recorded while trying to solve one of Piaget's weight seriation tasks. Detailed protocol analyses and computer simulations of the behavior of three children at different stages of intellectual development are presented. The programs, organized as systems of production rules, simulate the Ss' behavior in detail. They show how intellectual development is related to the Ss' (a) progressive sophistication in structuring their environment, (b) better use of memory, (c) span for drawing inferences, and (d) initial conception of what a seriation is, as reflected in such parameters as the adequacy of their stop rules and single vs. multiple block comparisons. Two additional experiments (N=35) compare length with weight seriation; access to information is controlled in one of the length seriation conditions. Results indicate that the typically observed décalage in performance on the two tasks largely disappears when a systematic effort is made to render the information-processing requirements of the two tasks isomorphic. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Gamma radiation effects on RF MEMS capacitive switches

Dielectric-based RF MEMS capacitive switches were fabricated and characterized for their response to dielectric charging, thermal storage and cycling and to total dose gamma irradiations. The evolution of the switch electromechanical and RF characteristics (actuation and releasing voltages, insertion losses, isolation) were evaluated as a function of the applied stress (temperature or total ionizing dose). It is indicated that the thermal stress has a relatively minor impact on the switches (the switches remained functional with nearly the same electrical properties). Under our particular test conditions, C(V) and S-parameters measurements show that gamma radiation has low to moderate effects on the components behavior.     

消除无线调制解调器中的PCMCIA功率限制

引言 支持移动计算要求的无线网络接入的需求正在迅速增长,移动通信全球系统（GSM）电信网络,以及引中出来的通用分组无线业务（GPRS）无处不在。工程设计人员已开始转向PCMCIA总线,将其作为实现GSM调制解调器设计的一种解决方案。