Phosphatidic acid (PtdOH) and lysophosphatidic acid (lysoPtdOH) have been shown to enhance T-lymphocyte function. However,
the FA preference and influence of acyl-CoA binding proteins on lysoPtdOH and PtdOH biosynthesis are not known. Therefore,
we determined glycerol-3-phosphate acyltransferase (GPAT) and lysophosphatidic acid acyltransferase (LAT) activity in rat
T-lymphocyte and liver membrane preparations in the presence of palmitoyl-CoA and oleoyl-CoA with or without BSA. We found
two different properties of GPAT and LAT in whole T-lymphocyte membrane preparations relative to liver. First, T-lymphocyte
basal GPAT and LAT activities were similar, whereas in liver membranes LAT activity was 10-fold higher than GPAT. Second,
T-lymphocyte LAT, but not GPAT, activity was inducible (fivefold) by the addition of albumin in the presence of palmitoyl-CoA
but not oleoyl-CoA. In contrast, albumin stimulated GPAT, but not LAT, activity in liver membranes in the presence of palmitoyl-CoA.
These results show, for the first time, that T-lymphocyte LAT activity can be increased by the presence of an acyl-CoA binding
protein, which may indicate a new important control mechanism for regulating intracellular lysoPtdOH and PtdOH levels in T-lymphocytes. 相似文献
A series of N-alkylacetonitrilium ions were characterized by IR spectroscopy on an activated H-ZSM-5 sample pretreated by CD3CN with various adsorbed olefins. Two kinds of(CN) band were evidenced according to whether the carbenium ion is secondary (2387 cm–1) or tertiary (2376 cm–1). The method is able to detect the isomerization intermediates of cyclohexene into methyl-cyclopentene. 相似文献
The world has been challenged since late 2019 by COVID-19. Higher education institutions have faced various challenges in adapting online education to control the pandemic spread of COVID-19. The present study aims to conduct a survey study through the interview and scrutinizing the literature to find the key challenges. Subsequently, an integrated MCDM framework, including Stepwise Weight Assessment Ratio Analysis (SWARA) and Multiple Objective Optimization based on Ratio Analysis plus Full Multiplicative Form (MULTIMOORA), is developed. The SWARA procedure is applied to the analysis and assesses the challenges to adapt the online education during the COVID-19 outbreak, and the MULTIMOORA approach is utilized to rank the higher education institutions on hesitant fuzzy sets. Further, an illustrative case study is considered to express the proposed idea's feasibility and efficacy in real-world decision-making. Finally, the obtained result is compared with other existing approaches, confirming the proposed framework's strength and steadiness. The identified challenges were systemic, pedagogical, and psychological challenges, while the analysis results found that the pedagogical challenges, including the lack of experience and student engagement, were the main essential challenges to adapting online education in higher education institutions during the COVID-19 outbreak.
The problem tackled in this article consists in associating perceived objects detected at a certain time with known objects previously detected, knowing uncertain and imprecise information regarding the association of each perceived objects with each known objects. For instance, this problem can occur during the association step of an obstacle tracking process, especially in the context of vehicle driving aid. A contribution in the modeling of this association problem in the belief function framework is introduced. By interpreting belief functions as weighted opinions according to the Transferable Belief Model semantics, pieces of information regarding the association of known objects and perceived objects can be expressed in a common global space of association to be combined by the conjunctive rule of combination, and a decision making process using the pignistic transformation can be made. This approach is validated on real data. 相似文献
The Clean Seas project focused on the role that existing Earth observing satellites might play in monitoring marine pollution. Results are presented here from August 1997, for the North Sea test site, using sea surface temperature (SST), colour and synthetic aperture radar (SAR) images in conjunction with a hydrodynamic model. There was good correlation between data sources, e.g. between SST and ERS-2 SAR images. Both datasets showed the development of fine plume structures close to the Rhine outflow, apparently associated with the outflow, and possibly caused by tidal pulsing of the Rhine Plume. The model reproduced general temperature and sediment distributions well, but fine structures were not reproduced. Model sediment distribution patterns were verified using ‘chlorophyll concentration’ data from colour sensors, representative of sediment concentration in turbid water. In conjunction with the visible channels of the Advanced Very High Resolution Radiometer and Along-Track Scanning Radiometer, they give an uncalibrated measure of the sediment load. The model gives a more complete picture of the temporal dispersion of the Rhine Plume over time than is evident from the remotely sensed data alone. 相似文献
During the transient phase of filling a casting running system, surface turbulence can cause the entrainment of oxide films
into the bulk liquid. Research has shown that these are detrimental to the material’s integrity. Common mechanisms for this
entrainment include returning waves, arising during filling of the runner bar, and plunging jets, found when pouring into
a basin. One of these, the returning wave, has been studied in greater depth, using real-time X-ray and process modeling techniques
alongside the application of physical principals. It has been concluded that when developed, returning waves cannot attain
the more stable and less entraining tranquil flow regime desirable in the running system of castings. 相似文献
We conducted a comparative evaluation of the Chlamydiazyme (Abbott Laboratories), PACE 2 (Gen-Probe), and AMP-CT (Gen-Probe) assays for the detection of Chlamydia trachomatis in endocervical samples. Specimens from 787 females were included in the study. The sensitivities of the PACE 2 and Chlamydiazyme assays in comparison to the results of the AMP-CT assay were 79.3 and 63.4%, respectively. The specificities of the Chlamydiazyme and PACE 2 assays were 100%. All of the positive specimens detected in this study were positive by the AMP-CT assay. On the basis of the final results of the comparison, the prevalence of C. trachomatis in the population was 10.4%. Retesting of specimens whose results were in the intermediate zone by the PACE 2 assay by a probe competition assay identified some additional true-positive specimens. Amplification assay testing of such specimens did not significantly increase the yield. The majority of specimens which tested positive by the AMP-CT assay only were not in the intermediate zone by the PACE 2 assay. We were unable to identify demographic or clinical factors which could predict those individuals who tested positive by amplified tests but not by nonamplified tests. The Gen-Probe PACE 2 assay proved to be superior to the Chlamydiazyme assay for the screening and diagnosis of C. trachomatis infections in female endocervical specimens. 相似文献