Analysis of reflectance spectra of tropical seagrass species and their value for mapping using multispectral satellite images

ABSTRACT

Mapping of the distribution of individual seagrass species is essential for any attempts to manage seagrass ecosystems. It is therefore important to understand how the spectra of different seagrass species vary, in order to establish their unique absorption features and how these can be utilised for mapping by making use of remote-sensing images. This paper presents measurements of the reflectance spectra between 400 and 900 nm for nine tropical species of seagrass. Continuum removal and multispectral resampling procedures were applied to the spectra. Dendrogram analysis was carried out to identify species clustering as the basis for a mapping scheme. Spectral Angle Mapper (SAM) and Spectral Information Divergence (SID) approaches were employed for the classification of seagrass species using WorldView-2 images and measured spectra as the input endmember. Classification Tree Analysis (CTA) and an image segmentation approach using CTA (Object-Based Image Analysis – OBIA) were performed as a means of comparison. The results indicate that the absorption features and overall shape of the spectra for all seagrass species are relatively similar, and implied that the major differences are attributable to the absolute reflectance values. Consequently, SAM and SID produced results of low accuracy (<30%), whereas, CTA and OBIA delivered results exhibiting higher accuracy (60–92%). The use of a spectral-based classification algorithm was ineffective for the classification and mapping of seagrass species using multispectral images. The utilisation of absolute reflectance values was beneficial for the classification of seagrass species having similar spectral shape.     

Mechanism of interaction of digitalis with estradiol binding sites in rat uteri

Digitalis preparations have a weak estrogenic effect in man. The data in animals are equivocal. We have studied.the biologic effect of both digitoxin and digoxin on the rat uterus in vivo and the interaction of these drugs with the rat uterus estrogen receptor in vitro. Digitoxin and estradiol significantly increased the uterine weight of immature rats, while digoxin did not. The interaction of digitoxin and digoxin with the rat uterus estrogen cytosol receptor was studied using protamine sulfate precipitation and dextran-coated charcoal (DCC) assays. Both methods gave a Kd for the estradiol-receptor interaction between 0.8-3.1 X 10(-9) M (n = 20). Digitoxin at concentrations of 0.5-2.0 X 10(-6) M significantly inhibited the binding of estradiol to the specific or saturable binding sites with minimal inhibition of hormonal binding to nonspecific sites. The binding was competitive with a calculated Ki for digitoxin of 5.2-7.8 X 10(-7) M (n = 18). Digoxin failed to inhibit estradiol binding to the receptor protein in vitro. We conclude that digitoxin probably acts directly as a weak estrogen and that this effect probably explains the estrogen-like side effects seen with digitoxin therapy in man.