Cervid Prion Protein Polymorphisms: Role in Chronic Wasting Disease Pathogenesis

Chronic wasting disease (CWD) is a prion disease found in both free-ranging and farmed cervids. Susceptibility of these animals to CWD is governed by various exogenous and endogenous factors. Past studies have demonstrated that polymorphisms within the prion protein (PrP) sequence itself affect an animal’s susceptibility to CWD. PrP polymorphisms can modulate CWD pathogenesis in two ways: the ability of the endogenous prion protein (PrP^C) to convert into infectious prions (PrP^Sc) or it can give rise to novel prion strains. In vivo studies in susceptible cervids, complemented by studies in transgenic mice expressing the corresponding cervid PrP sequence, show that each polymorphism has distinct effects on both PrP^C and PrP^Sc. It is not entirely clear how these polymorphisms are responsible for these effects, but in vitro studies suggest they play a role in modifying PrP epitopes crucial for PrP^C to PrP^Sc conversion and determining PrP^C stability. PrP polymorphisms are unique to one or two cervid species and most confer a certain degree of reduced susceptibility to CWD. However, to date, there are no reports of polymorphic cervid PrP alleles providing absolute resistance to CWD. Studies on polymorphisms have focused on those found in CWD-endemic areas, with the hope that understanding the role of an animal’s genetics in CWD can help to predict, contain, or prevent transmission of CWD.     

Auto-generation of IEC standard PLC code using t-MPSG

The objective of this paper is to reduce the development time of a PLC (Programmable Logic Controller) by automating the task of code generation. For this purpose, we applied t-MPSG (Timed-Message Based Part State Graph). The t-MPSG is an extended finite state automata used to model and generate an execution module for a real-time shop floor controller system. In our proposed method, t-MPSG is used to model the formal specification of the controller system that can be translated into textual structure. After the verification of the t-MPSG model, it can be used as an input to the plc-builder tool. The plc-builder tool is an extended version of a conventional MPSG simulator. It can be used to translate the textual structure of the t-MPSG into an IEC standard PLC code. Finally, the generated code can be downloaded to a PLC emulator or a PLC device for the purpose of simulation and execution. The similarity in the hierarchical structure of the t-MPSG and the IEC standard PLC program has made it convenient to transform from one form to another. Furthermore, an illustration of the methodology to auto-generate IEC standard PLC code using t-MPSG is explained with a suitable example. Recommended by Editorial Board member Young Soo Suh under the direction of Editor Jae Weon Choi. This work was partially supported by Defense Acquisition Program Administration and Agency for Defense Development under the contract (UD080042AD). Devinder Thapa is a Postdoc Research Fellow in the Department of Industrial & information systems at Ajou University, Korea. He completed his Ph.D. from Ajou University in Industrial and Information Systems Engineering. His area of research is related to manufacturing automation and intelligent decision support systems. Chang Mok Park is a Professor in the Department of Technology & Systems Management at Induk Institute of Technology. He completed his Ph.D. in 2002 from Ajou University in Industrial Engineering. His research interest is related to manufacturing optimization, discrete event system simulation and signal analysis. Sang C. Park is an Associate Professor in the Department of Industrial & Information Systems Engineering at Ajou University. He received his B.S., M.S., and Ph.D. degrees from KAIST in 1994, 1996, and 2000, respectively, all in Industrial Engineering. His research interests include geometric algorithms in CAD/CAM, process planning, engineering knowledge management, and discrete event system simulation. Gi-Nam Wang is the Head and a Professor in the Department of Industrial & Information Systems Engineering at Ajou University, Korea. He completed his Ph.D. in 1992 from Texas A&M University, in Industrial Engineering. He has worked as Visiting Professor at University of Texas at Austin during 2000–2001. His area of research is related to Intelligent Information & manufacturing systems, system integration & automation, e-Business solutions and image processing.     

Urban growth modeling of Kathmandu metropolitan region, Nepal

The complexity of urban system requires integrated tools and techniques to understand the spatial process of urban development and project the future scenarios. This research aims to simulate urban growth patterns in Kathmandu metropolitan region in Nepal. The region, surrounded by complex mountainous terrain, has very limited land resources for new developments. As similar to many cities of the developing world, it has been facing rapid population growth and daunting environmental problems. Three time series land use maps in a fine-scale (30 m resolution), derived from satellite remote sensing, for the last three decades of the 20th century were used to clarify the spatial process of urbanization. Based on the historical experiences of the land use transitions, we adopted weight of evidence method integrated in cellular automata framework for predicting the future spatial patterns of urban growth. We extrapolated urban development patterns to 2010 and 2020 under the current scenario across the metropolitan region. Depending on local characteristics and land cover transition rates, this model produced noticeable spatial pattern of changes in the region. Based on the extrapolated spatial patterns, the urban development in the Kathmandu valley will continue through both in-filling in existing urban areas and outward rapid expansion toward the east and south directions. Overall development will be greatly affected by the existing urban space, transportation network, and topographic complexity.     

Improving hydrophobicity of polyurethane by PTFE incorporation

A simple and facile method was established of incorporating polytetrafluoroethylene (PTFE) on to polyurethane (PU) to improve hydrophobicity of PU by incorporating low levels of fluorine at a molecular level. Nanocomposites were made by preparing PU in the presence of PTFE using seeded miniemulsion polymerization method. The resulting PTFE/PU nanocomposites were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectrometry, differential scanning calorimetric, and thermo gravimetric analysis (TGA). FTIR and TEM indicated changes observed in their structure, size and morphology. The water contact angle of PTFE/PU nanocomposite films increased with increasing amount of PTFE and more on blending with silica nanoparticles but a slight decrease in thermal stabilities of SiO₂/PTFE/PU nanocomposites were noticed. The hydrophobicity imparted by PTFE to PU surface was found to be at its best for 1 : 2 PTFE/PU latex film. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42779.     

Phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the Eastern Himalayas

Sukako maacha, gnuchi, sidra and sukuti are traditional smoked and sun-dried fish products of the Eastern Himalayan regions of Nepal and India. A total of 40 samples of sukako maacha (14), gnuchi (6), sidra (10) and sukuti (10) were collected and were analysed for microbial load. Population of lactic acid bacteria (LAB) as well as aerobic mesophilic counts ranged from 4.7-8.3 to 5.1-8.5 log cfu g(-1), respectively. A total of 189 strains of LAB were isolated from sukako maacha, gnuchi, sidra and sukuti samples, out of which 171 strains were cocci and 15 strains, were heterofermentative lactobacilli. LAB were identified on the basis of phenotypic characters including API system as Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactococcus plantarum, Leuconostoc mesenteroides, Enterococcus faecium, Enterococcus faecalis, Pediococcus pentosaceus and Weissella confusa. LAB strains produced a wide spectrum of enzymes. Some strains of LAB showed antagonistic properties against pathogenic strains. None of the strains produced biogenic amines in the method applied. This paper is the first report on the microbial composition, mostly lactic acid bacteria, of traditionally processed fish products of Eastern Himalayas.     

Identification and enzymatic profiles of the predominant lactic acid bacteria isolated from soft‐variety Chhurpi,a traditional cheese typical of the Sikkim Himalayas

Abstract

This paper is the first report on the microbial population and predominant lactic acid bacteria (LAB) of two soft‐varieties (mild and strong flavoured) of chhurpi., a traditional cheese product of the Sikkim Himalayas. The enzymatic profiles and percentage hydrophobicity (as one criterion of potential adhesion capability) of the predominant LAB were also studied. The LAB, yeasts and viable mesophilic microbial numbers ranged from 7.6–7.9, 7.0–7.4 and 7.7–8.0 log₁₀ cfu/g respectively. The predominant LAB were identified as Lactobacillus plantarum, Lb. curvatus, Lb. fermentum, Lb. paracasei subsp. pseudoplcmtarum and Leuconostoc mesenteroides. They produced a wide spectrum of enzymes and also exhibited similar patterns of enzymatic activity between species. In comparison to the peptidases, they showed relatively weak esterase and lipase activities. No proteinase activity was detected. Most strains had a high degree of hydrophobicity. The results are discussed in relation to the possible role of the strains in food processing.     

Lignite aided dewatering of digested sewage sludge

Mechanical dewatering is commonly used to increase the solids content of municipal sludge prior to its disposal. However, if the rate of filtration is slow, mechanical dewatering can be expensive. In this study, the use of lignite to improve the sludge dewatering is investigated. The effectiveness of lignite conditioning of polyelectrolyte-flocculated sludge is examined using mechanical compression tests. Results show that lignite conditioning in conjunction with polyelectrolyte flocculation gives much better dewatering than the polyelectrolyte flocculation alone. Using Darcy's filtration theory, the specific cake resistance and permeability of the compressed cakes are obtained. Both of these parameters are significantly improved after lignite conditioning. Mercury porosimetry tests on compressed cakes show that the porosity of the lignite-conditioned sludge cake is much higher than that of the polyelectrolyte-flocculated sludge and it increases with increasing doses of lignite. The mercury porosimetry results show that the lignite pore volume of pores greater than 0.5 μm are reduced with increasing sludge ratio indicating that sludge is trapped within these pores, whereas smaller pores are unaffected. The yield stress curves for sludge, lignite and sludge-lignite mixtures show that the sludge filter cake is very compressible, but the lignite-conditioned cake has a range of compressibility which although more than lignite indicate that the cake is relatively incompressible at low pressures. Thus, lignite conditioning acts to maintain the permeability of the filter cake during compression dewatering by resisting cake compression. This leads to a trade-off between the rate of dewatering and the solids content of the compressed cake. With lignite conditioning, the dewatering rate can be increased by a factor of five for the same degree of water removal.     

Prediction of semiconducting behavior in minority spin of Co2CrZ（Z=Ga,Ge,As）:LSDA

正Volume optimization was performed to obtain the theoretical lattice constants by using the generalized gradient approximation(GGA).The electronic and magnetic properties of Heusler alloys Co_2CrZ(Z = Ga,Ge, As) were investigated by using local spin density approximation(LSDA).Amongst the systems under investigation, Co_2CrGe and Co_2CrGa give 100%spin polarization at the Fermi level(E_f)- Co_2CrGe and Co_2CrGa are the most stable half-metallic ferromagnets(HMFs);their E_F lie exactly at the gap of 0.24 eV and 0.38 eV,respectively,in the spin-down channel.Even though Co_2CrAs gives a distinct and bigger gap as compared to Co_2CrGa and Co_2CrGe, its E_F is not located at the middle of the gap in the spin-down channel.We have also found that the total magnetic moments increase as the Z goes from Ga to As.The calculated density of states and band structures show the HMF character for Co_2CrGe and Co_2CrGa.     

Identification of yeast strains isolated from marcha in Sikkim, a microbial starter for amylolytic fermentation

Marcha or murcha is a traditional amylolytic starter used to produce sweet-sour alcoholic drinks, commonly called jaanr in the Himalayan regions of India, Nepal, Bhutan, and Tibet (China). The aim of this study was to examine the microflora of marcha collected from Sikkim in India, focusing on yeast flora and their roles. Twenty yeast strains were isolated from six samples of marcha and identified by genetic and phenotypic methods. They were first classified into four groups (Group I, II, III, and IV) based on physiological features using an API test. Phylogenetic, morphological, and physiological characterization identified the isolates as Saccharomyces bayanus (Group I); Candida glabrata (Group II); Pichia anomala (Group III); and Saccharomycopsis fibuligera, Saccharomycopsis capsularis, and Pichia burtonii (Group IV). Among them, the Group I, II, and III strains produced ethanol. The isolates of Group IV had high amylolytic activity. Because all marcha samples tested contained both starch degraders and ethanol producers, it was hypothesized that all four groups of yeast (Group I, II, III, and IV) contribute to starch-based alcohol fermentation.     

A DFT study of BeX (X = S,Se, Te) semiconductor: Modified Becke Johnson (mBJ) potential

The electronic, optical and elastic properties of BeX were performed within full potential liberalized augmented plane wave method based on density functional theory (DFT). Generalized gradient approximation (GGA) and modified Becke Johnson (TB-mBJ) potential were used for exchange correlation. The mBJ gives improved band gap as compare to GGA and in close agreement with the experimental results. The present band gaps of BeS, BeSe and BeTe calculated within mBJ are 4.40, 4.0 and 2.40 eV respectively.