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Li-Jane Shih Jia-Yu Wang Jing-Yao Jheng An-Ci Siao Yen-Yue Lin Yi-Wei Tsuei Yow-Chii Kuo Chih-Pin Chuu Yung-Hsi Kao 《International journal of molecular sciences》2020,21(23)
Prostate cancer (PCa) is a reproductive system cancer in elderly men. We investigated the effects of betel nut arecoline on the growth of normal and cancerous prostate cells. Normal RWPE-1 prostate epithelial cells, androgen-independent PC-3 PCa cells, and androgen-dependent LNCaP PCa cells were used. Arecoline inhibited their growth in dose- and time-dependent manners. Arecoline caused RWPE-1 and PC-3 cell cycle arrest in the G2/M phase and LNCaP cell arrest in the G0/G1 phase. In RWPE-1 cells, arecoline increased the expression of cyclin-dependent kinase (CDK)-1, p21, and cyclins B1 and D3, decreased the expression of CDK2, and had no effects on CDK4 and cyclin D1 expression. In PC-3 cells, arecoline decreased CDK1, CDK2, CDK4, p21, p27, and cyclin D1 and D3 protein expression and increased cyclin B1 protein expression. In LNCaP cells, arecoline decreased CDK2, CDK4, and cyclin D1 expression; increased p21, p27, and cyclin D3 expression; had no effects on CDK1 and cyclin B1 expression. The antioxidant N-acetylcysteine blocked the arecoline-induced increase in reactive oxygen species production, decreased cell viability, altered the cell cycle, and changed the cell cycle regulatory protein levels. Thus, arecoline oxidant exerts differential effects on the cell cycle through modulations of regulatory proteins. 相似文献
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Yen-Jung Chou Wei-Chih Kan Chieh-Min Chang Yi-Jen Peng Hsien-Yi Wang Wen-Chun Yu Yu-Hsuan Cheng Yu-Rou Jhang Hsia-Wei Liu Jiunn-Jye Chuu 《International journal of molecular sciences》2016,17(9)
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease in diabetes mellitus. Oxidative stress, insulin resistance and pro-inflammatory cytokines have been shown to play an important role in pathogeneses of renal damage on type 2 diabetes mellitus (DM). Inonotus obliquus (IO) is a white rot fungus that belongs to the family Hymenochaetaceae; it has been used as an edible mushroom and exhibits many biological activities including anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic properties. Especially the water-soluble Inonotus obliquus polysaccharides (IOPs) have been previously reported to significantly inhibit LPS-induced inflammatory cytokines in mice and protect from streptozotocin (STZ)-induced diabetic rats. In order to identify the nephroprotective effects of low molecular weight of IOP fraction (LIOP), from the fruiting bodies of Inonotus obliquus, high-fat diet (HFD) plus STZ-induced type 2-like diabetic nephropathy C57BL/6 mice were investigated in this study. Our data showed that eight weeks of administration of 10–100 kDa, LIOP (300 mg/kg) had progressively increased their sensitivity to glucose (less insulin tolerance), reduced triglyceride levels, elevated the HDL/LDL ratio and decreased urinary albumin/creatinine ratio(ACR) compared to the control group. By pathological and immunohistochemical examinations, it was indicated that LIOP can restore the integrity of the glomerular capsules and increase the numbers of glomerular mesangial cells, associated with decreased expression of TGF-β on renal cortex in mice. Consistently, three days of LIOP (100 μg/mL) incubation also provided protection against STZ + AGEs-induced glucotoxicity in renal tubular cells (LLC-PK1), while the levels of NF-κB and TGF-β expression significantly decreased in a dose-dependent manner. Our findings demonstrate that LIOP treatment could ameliorate glucolipotoxicity-induced renal fibrosis, possibly partly via the inhibition of NF-κB/TGF-β1 signaling pathway in diabetic nephropathy mice. 相似文献
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Ying-Yu Kuo Wai-Tim Jim Liang-Cheng Su Chi-Jung Chung Ching-Yu Lin Chieh Huo Jen-Chih Tseng Shih-Han Huang Chih-Jen Lai Bo-Chih Chen Bi-Juan Wang Tzu-Min Chan Hui-Ping Lin Wun-Shaing Wayne Chang Chuang-Rung Chang Chih-Pin Chuu 《International journal of molecular sciences》2015,16(5):10748-10766
Head and neck cancers, which affect 650,000 people and cause 350,000 deaths per year, is the sixth leading cancer by cancer incidence and eighth by cancer-related death worldwide. Oral cancer is the most common type of head and neck cancer. More than 90% of oral cancers are oral and oropharyngeal squamous cell carcinoma (OSCC). The overall five-year survival rate of OSCC patients is approximately 63%, which is due to the low response rate to current therapeutic drugs. In this review we discuss the possibility of using caffeic acid phenethyl ester (CAPE) as an alternative treatment for oral cancer. CAPE is a strong antioxidant extracted from honeybee hive propolis. Recent studies indicate that CAPE treatment can effectively suppress the proliferation, survival, and metastasis of oral cancer cells. CAPE treatment inhibits Akt signaling, cell cycle regulatory proteins, NF-κB function, as well as activity of matrix metalloproteinase (MMPs), epidermal growth factor receptor (EGFR), and Cyclooxygenase-2 (COX-2). Therefore, CAPE treatment induces cell cycle arrest and apoptosis in oral cancer cells. According to the evidence that aberrations in the EGFR/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling, NF-κB function, COX-2 activity, and MMPs activity are frequently found in oral cancers, and that the phosphorylation of Akt, EGFR, and COX-2 correlates to oral cancer patient survival and clinical progression, we believe that CAPE treatment will be useful for treatment of advanced oral cancer patients. 相似文献
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Dynamic dielectric analysis was used to analyze the miscibility of poly(vinyl acetate) and natural rubber and the effect of moisture on the dielectric properties of the polymer blend. Two separate dielectric relaxations were observed within the temperature range studied. The high-temperature relaxation was a result of the glass transition of poly(vinyl acetate), whereas the low-temperature relaxation was attributed to that of natural rubber. The peak temperatures of ε″ of the high-temperature relaxation decreased with increasing aging time, while the magnitudes of the ε″ peaks increased with increasing days of aging. The peak temperatures of ε″ of the low-temperature relaxation stayed constant, while the magnitudes of the ε″ peaks increased with increasing days of aging. This indicates that both polymers are responsible for the sorption of moisture; however, water is an effective plasticizer only for poly(vinyl acetate). Anomalous increases in the magnitudes of ε″, especially in the low frequency sides, of the Argand diagrams were explained by Maxwell–Wagner–Sillar (MWS) polarization at component interfaces. The oscillator strength, relaxation time, and the two distribution parameters, A and B, calculated from the skewed circular are equation, were affected with days of aging. 相似文献
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Yutsung Tsai Zhaodong Chu Yimo Han Chih‐Piao Chuu Di Wu Alex Johnson Fei Cheng Mei‐Yin Chou David A. Muller Xiaoqin Li Keji Lai Chih‐Kang Shih 《Advanced materials (Deerfield Beach, Fla.)》2017,29(41)
Semiconductor heterostructures have played a critical role as the enabler for new science and technology. The emergence of transition‐metal dichalcogenides (TMDs) as atomically thin semiconductors has opened new frontiers in semiconductor heterostructures either by stacking different TMDs to form vertical heterojunctions or by stitching them laterally to form lateral heterojunctions via direct growth. In conventional semiconductor heterostructures, the design of multijunctions is critical to achieve carrier confinement. Analogously, successful synthesis of a monolayer WS2/WS2(1?x )Se2x /WS2 multijunction lateral heterostructure via direct growth by chemical vapor deposition is reported. The grown structures are characterized by Raman, photoluminescence, and annular dark‐field scanning transmission electron microscopy to determine their lateral compositional profile. More importantly, using microwave impedance microscopy, it is demonstrated that the local photoconductivity in the alloy region can be tailored and enhanced by two orders of magnitude over pure WS2. Finite element analysis confirms that this effect is due to the carrier diffusion and confinement into the alloy region. This work exemplifies the technological potential of atomically thin lateral heterostructures in optoelectronic applications. 相似文献