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排序方式: 共有1021条查询结果,搜索用时 62 毫秒
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Dr. Yibin Zhang Dr. Shuai Xia Shulin Wan Tessa E. Steenwinkel Tara Vohs Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2021,22(13):2282-2291
Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. 相似文献
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ABSTRACT Design and implementation of an effective dissemination programme for decentralised renewable energy system necessitate an accurate estimate of its utilisation potential. Hence, in this study, an attempt has been made to develop frameworks to estimate the utilisation potential of decentralised renewable energy systems in the state of Uttarakhand in India. Estimations imply large resource, technical and economic potentials of the domestic solar water heater, solar home system, solar lantern, family size biogas plant and improved biomass cookstove in Uttarakhand. With higher impact on the purchasing power of households, prevailing soft loan scheme has been found to be more appropriate than a capital subsidy for promoting the usage of decentralised renewable energy systems. 相似文献
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Industries in the US continue to lose ground in international competition. If IS is to help change that trend, it will have to become more creative. Creativity improvement programs are being introduced in other areas of the company— now is the time for IS organizations to follow suit. 相似文献
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A 73-year-old HTLV-1 infected male developed overt ATL following 3-years observation, and the clonality of HTLV-1 infected cells changed before overt ATL onset. 10 micrograms of DNA, extracted from mononuclear cells was digested with PstI, and the clonal proliferation of HTLV-1 infected cells was examined by Southern blotting with LTR probe. 4 bands were observed 3 years prior to ATL onset, and 2 different bands were detected 2 years and 6 months later. This suggests that in some ATL cases, the clonality of monoclonally proliferated HTLV-1 infected cells may be changeable before overt ATL onset. 相似文献
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Flynn P.J. Jain A.K. 《IEEE transactions on pattern analysis and machine intelligence》1991,13(10):1066-1075
BONSAI, a model-based 3D object recognition system, is described. It identifies and localizes 3D objects in range images of one or more parts that have been designed on a computer-aided-design (CAD) system. Recognition is performed via constrained search of the interpretation tree, using unary and binary constraints (derived automatically from the CAD models) to prune the search space. Attention is focused on the recognition procedure, but the model-building, image acquisition, and segmentation procedures are also outlined. Experiments with over 200 images demonstrate that the constrained search approach to 3D object recognition has an accuracy comparable to that of previous systems 相似文献
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A Region Ensemble for 3-D Face Recognition 总被引:1,自引:0,他引:1
Faltemier T.C. Bowyer K.W. Flynn P.J. 《Information Forensics and Security, IEEE Transactions on》2008,3(1):62-73
In this paper, we introduce a new system for 3D face recognition based on the fusion of results from a committee of regions that have been independently matched. Experimental results demonstrate that using 28 small regions on the face allow for the highest level of 3D face recognition. Score-based fusion is performed on the individual region match scores and experimental results show that the Borda count and consensus voting methods yield higher performance than the standard sum, product, and min fusion rules. In addition, results are reported that demonstrate the robustness of our algorithm by simulating large holes and artifacts in images. To our knowledge, no other work has been published that uses a large number of 3D face regions for high-performance face matching. Rank one recognition rates of 97.2% and verification rates of 93.2% at a 0.1% false accept rate are reported and compared to other methods published on the face recognition grand challenge v2 data set. 相似文献