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排序方式: 共有47条查询结果,搜索用时 15 毫秒
1.
Benjamin Alexander Tyrone L. Daulton Guy M. Genin Justin Lipner Jill D. Pasteris Brigitte Wopenka Stavros Thomopoulos 《Journal of the Royal Society Interface》2012,9(73):1774-1786
The nanometre-scale structure of collagen and bioapatite within bone establishes bone''s physical properties, including strength and toughness. However, the nanostructural organization within bone is not well known and is debated. Widely accepted models hypothesize that apatite mineral (‘bioapatite’) is present predominantly inside collagen fibrils: in ‘gap channels’ between abutting collagen molecules, and in ‘intermolecular spaces’ between adjacent collagen molecules. However, recent studies report evidence of substantial extrafibrillar bioapatite, challenging this hypothesis. We studied the nanostructure of bioapatite and collagen in mouse bones by scanning transmission electron microscopy (STEM) using electron energy loss spectroscopy and high-angle annular dark-field imaging. Additionally, we developed a steric model to estimate the packing density of bioapatite within gap channels. Our steric model and STEM results constrain the fraction of total bioapatite in bone that is distributed within fibrils at less than or equal to 0.42 inside gap channels and less than or equal to 0.28 inside intermolecular overlap regions. Therefore, a significant fraction of bone''s bioapatite (greater than or equal to 0.3) must be external to the fibrils. Furthermore, we observe extrafibrillar bioapatite between non-mineralized collagen fibrils, suggesting that initial bioapatite nucleation and growth are not confined to the gap channels as hypothesized in some models. These results have important implications for the mechanics of partially mineralized and developing tissues. 相似文献
2.
Jean-Rémi Bourguet Rallou Thomopoulos Marie-Laure Mugnier Joël Abécassis 《Expert systems with applications》2013,40(11):4539-4546
Argumentation is a relatively new research area in Artificial Intelligence. Since the early 1980s, its use has been investigated in various frameworks. We propose a general model for recommendation-based argumentation by extending Dung’s seminal argumentation system. This approach is applied to analyse argumentation on food quality in a public health policy. Cereal products, and more specifically bread, are used by decision makers as a healthy lever to fight against diseases such as obesity or diabetes. Our model outputs new recommendations based on stakeholder’s argumentation by targeting some specific audiences. 相似文献
3.
Stelios C. A. Thomopoulos 《野外机器人技术杂志》1990,7(3):337-372
The problem of sensor integration and data fusion is addressed. We consider the problem of combining information from diversified sources in a coherent fashion. We assume that information from various sensors may be available in different forms at the fusion. For example, data from infrared (IR) sensors may be combined with range radar (RR) data and further combined with visual images. In each case, data and information from different sensors are presented in a different format which may not be directly compatible for all sensors. Part of the available information may be in the form of attributes and part in the form of dynamical measurements. A generalized evidence processing theory and an architecture for sensor integration and data fusion that accommodates diversified sources of information are presented. Data (or, more generically, information) fusion may take place at different levels, such as the level of dynamics, the level of attributes, and the level of evidence. The common and different aspects of fusion at the different levels are investigated and several practical examples of real world data fusion problems are discussed. 相似文献
4.
John J. Boyle Maiko Kume Matthew A. Wyczalkowski Larry A. Taber Robert B. Pless Younan Xia Guy M. Genin Stavros Thomopoulos 《Journal of the Royal Society Interface》2014,11(100)
When mechanical factors underlie growth, development, disease or healing, they often function through local regions of tissue where deformation is highly concentrated. Current optical techniques to estimate deformation can lack precision and accuracy in such regions due to challenges in distinguishing a region of concentrated deformation from an error in displacement tracking. Here, we present a simple and general technique for improving the accuracy and precision of strain estimation and an associated technique for distinguishing a concentrated deformation from a tracking error. The strain estimation technique improves accuracy relative to other state-of-the-art algorithms by directly estimating strain fields without first estimating displacements, resulting in a very simple method and low computational cost. The technique for identifying local elevation of strain enables for the first time the successful identification of the onset and consequences of local strain concentrating features such as cracks and tears in a highly strained tissue. We apply these new techniques to demonstrate a novel hypothesis in prenatal wound healing. More generally, the analytical methods we have developed provide a simple tool for quantifying the appearance and magnitude of localized deformation from a series of digital images across a broad range of disciplines. 相似文献
5.
Behzad Babaei Steven D. Abramowitch Elliot L. Elson Stavros Thomopoulos Guy M. Genin 《Journal of the Royal Society Interface》2015,12(113)
The viscoelastic behaviour of a biological material is central to its functioning and is an indicator of its health. The Fung quasi-linear viscoelastic (QLV) model, a standard tool for characterizing biological materials, provides excellent fits to most stress–relaxation data by imposing a simple form upon a material''s temporal relaxation spectrum. However, model identification is challenging because the Fung QLV model''s ‘box’-shaped relaxation spectrum, predominant in biomechanics applications, can provide an excellent fit even when it is not a reasonable representation of a material''s relaxation spectrum. Here, we present a robust and simple discrete approach for identifying a material''s temporal relaxation spectrum from stress–relaxation data in an unbiased way. Our ‘discrete QLV’ (DQLV) approach identifies ranges of time constants over which the Fung QLV model''s typical box spectrum provides an accurate representation of a particular material''s temporal relaxation spectrum, and is effective at providing a fit to this model. The DQLV spectrum also reveals when other forms or discrete time constants are more suitable than a box spectrum. After validating the approach against idealized and noisy data, we applied the methods to analyse medial collateral ligament stress–relaxation data and identify the strengths and weaknesses of an optimal Fung QLV fit. 相似文献
6.
7.
Maria Tzianou Giannis Thomopoulos Nikolaos Vourdas Kosmas Ellinas Evangelos Gogolides 《Advanced functional materials》2021,31(1):2006687
Fog formation decreases light transmission of optically clear materials. A promising approach to address this problem is to control the wetting properties of the material at extremes states, which requires imparting micro and nano morphology features on the surface. However, such features may affect the optical properties of the surface. In this work, superhydrophobic and superhydrophilic surfaces, with different morphology characteristics ranging from nanoscale to hierarchical micro-nanoscale are fabricated and evaluated in order to investigate which wetting extreme and surface morphology is more suitable to preserve the light-transmitting properties and exhibit antifogging functionalities. The performance of the aforementioned surfaces is compared for the first time in two different testing modes: under intense fog flow and no surface cooling, and under no-flow and surface cooling, which enhances dew condensation on the surfaces. It is demonstrated that superhydrophilic surfaces with nanoscale morphology maintain their optical transmittance under fog flow for more than 20 min. This duration is one of the longest reported in the literature revealing the long-term antifogging functionality of the proposed surfaces. Finally, by tailoring the morphology and the surface wetting properties, an optically switching surface (initially “milky” which becomes “clear”) when exposed to humidity is demonstrated. 相似文献
8.
The diagnosis of meningeal carcinomatosis hinges on the cytologic examination of cerebrospinal fluid (CSF), which has a known low sensitivity for the identification of malignant cells. Often only 'suspicious' or 'atypical' diagnoses can be rendered, and specimens are commonly unsatisfactory for evaluation due to poor morphologic preservation. Telomerase is widely expressed in most brain metastases, medulloblastomas, lymphomas, oligodendrogliomas, and is expressed focally in glioblastomas. Little is known about the level of telomerase expression in these tumors, except for brain metastases, where a four-fold variation in telomerase levels exists. In our laboratory, as few as ten carcinoma cells can be detected by a sensitive polymerase chain reaction-based assay, the telomeric repeat amplification protocol (TRAP), for telomerase, but it was unclear whether varying levels of telomerase expressed by different types of metastases would influence detection. Using the TRAP protocol, we studied 281 CSF samples from a wide variety of patients with neurologic and non-neurologic conditions for telomerase expression. An adjusted specificity of 90% and a sensitivity of 64% were achieved for detection of malignant cells in CSF by telomerase expression. The TRAP assay for telomerase detection may serve as an adjunct to the traditional examination of CSF. Neither previously documented four-fold variation in the levels of telomerase expression in brain metastases, high CSF protein levels nor high white blood cell counts precluded detection of malignant cells in CSF. 相似文献
9.
Inverse Opal Scaffolds with Gradations in Mineral Content for Spatial Control of Osteogenesis 下载免费PDF全文
Chunlei Zhu Jichuan Qiu Suphannee Pongkitwitoon Stavros Thomopoulos Younan Xia 《Advanced materials (Deerfield Beach, Fla.)》2018,30(29)
The design and fabrication of inverse opal scaffolds with gradations in mineral content to achieve spatial control of osteogenesis are described. The gradient in mineral content is established via the diffusion‐limited transport of hydroxyapatite nanoparticles in a closely packed lattice of gelatin microbeads. The mineral‐graded scaffold has an array of uniform pores and interconnected windows to facilitate efficient transport of nutrients and metabolic wastes, ensuring high cell viability. The graded distribution of mineral content can provide biochemical and mechanical cues for spatially regulating the osteogenic differentiation of adipose‐derived stromal cells. This new class of scaffolds holds promise for engineering the interfaces between mineralized and unmineralized tissues. 相似文献
10.
Chunlei Zhu Suphannee Pongkitwitoon Jichuan Qiu Stavros Thomopoulos Younan Xia 《Advanced materials (Deerfield Beach, Fla.)》2018,30(16)
A hierarchically structured scaffold is designed and fabricated for facilitating tendon‐to‐bone repair. The scaffold is composed of three regions with distinct functions: (i) an array of channels to guide the in‐growth of cells and aligned deposition of collagen fibers, as well as integration of the scaffold with the tendon side, (ii) a region with a gradient in mineral composition to facilitate stress transfer between tendon and bone, and (iii) a mineralized inverse opal region to promote the integration of the scaffold with the underlying bone. Cell culture experiments confirm that adipose‐derived stromal cells are able to infiltrate and proliferate through the entire thickness of the scaffold without compromised cell viability. The seeded stem cells exhibit directed differentiation into tenocytes and osteoblasts along the mineral gradient as a response to the gradient in Young's modulus. This novel scaffold holds great promise to promote the formation of a functional tendon‐to‐bone attachment by offering a structurally and compositionally appropriate microenvironment for healing. 相似文献