全文获取类型
收费全文 | 2062篇 |
免费 | 212篇 |
国内免费 | 7篇 |
专业分类
电工技术 | 40篇 |
综合类 | 11篇 |
化学工业 | 510篇 |
金属工艺 | 56篇 |
机械仪表 | 32篇 |
建筑科学 | 156篇 |
矿业工程 | 3篇 |
能源动力 | 52篇 |
轻工业 | 100篇 |
水利工程 | 9篇 |
无线电 | 236篇 |
一般工业技术 | 432篇 |
冶金工业 | 137篇 |
原子能技术 | 13篇 |
自动化技术 | 494篇 |
出版年
2024年 | 7篇 |
2023年 | 52篇 |
2022年 | 67篇 |
2021年 | 100篇 |
2020年 | 87篇 |
2019年 | 79篇 |
2018年 | 89篇 |
2017年 | 74篇 |
2016年 | 107篇 |
2015年 | 105篇 |
2014年 | 120篇 |
2013年 | 145篇 |
2012年 | 155篇 |
2011年 | 161篇 |
2010年 | 139篇 |
2009年 | 100篇 |
2008年 | 102篇 |
2007年 | 96篇 |
2006年 | 85篇 |
2005年 | 61篇 |
2004年 | 33篇 |
2003年 | 17篇 |
2002年 | 21篇 |
2001年 | 17篇 |
2000年 | 16篇 |
1999年 | 20篇 |
1998年 | 34篇 |
1997年 | 27篇 |
1996年 | 15篇 |
1995年 | 12篇 |
1994年 | 17篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 10篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1976年 | 10篇 |
1975年 | 6篇 |
1968年 | 3篇 |
1908年 | 6篇 |
1907年 | 3篇 |
排序方式: 共有2281条查询结果,搜索用时 156 毫秒
1.
Prof. Andrea Baier Dr. Anne Kokel Dr. William Horton Ewa Gizińska Dr. Garima Pandey Prof. Ryszard Szyszka Prof. Béla Török Prof. Marianna Török 《ChemMedChem》2021,16(12):1927-1932
A set of novel hydrazone derivatives were synthesized and analyzed for their biological activities. The compounds were tested for their inhibitory effect on the phosphorylating activity of the protein kinase CK2, and their antioxidant activity was also determined in three commonly used assays. The hydrazones were evaluated for their radical scavenging against the DPPH, ABTS and peroxyl radicals. Several compounds have been identified as good antioxidants as well as potent protein kinase CK2 inhibitors. Most hydrazones containing a 4-N(CH3)2 residue or perfluorinated phenyl rings showed high activity in the radical-scavenging assays and possess nanomolar IC50 values in the kinase assays. 相似文献
2.
Dr. Sandra Liebscher Dr. Sebastian Mathea Dr. Tobias Aumüller Dr. Andreas Pech Prof. Dr. Frank Bordusa 《Chembiochem : a European journal of chemical biology》2021,22(7):1201-1204
Fluorescent fusion proteins are powerful tools for studying biological processes in living cells, but universal application is limited due to the voluminous size of those tags, which might have an impact on the folding, localization or even the biological function of the target protein. The designed biocatalyst trypsiligase enables site-directed linkage of small-sized fluorescence dyes on the N terminus of integral target proteins located in the outer membrane of living cells through a stable native peptide bond. The function of the approach was tested by using the examples of covalent derivatization of the transmembrane proteins CD147 as well as the EGF receptor, both presented on human HeLa cells. Specific trypsiligase recognition of the site of linkage was mediated by the dipeptide sequence Arg-His added to the proteins’ native N termini, pointing outside the cell membrane. The labeling procedure takes only about 5 minutes, as demonstrated for couplings of the fluorescence dye tetramethyl rhodamine and the affinity label biotin as well. 相似文献
3.
Dr. Raysa Khan Tareque Dr. Storm Hassell-Hart Dr. Tobias Krojer Dr. Anthony Bradley Dr. Srikannathasan Velupillai Dr. Romain Talon Dr. Michael Fairhead Dr. Iain J. Day Kamlesh Bala Dr. Robert Felix Dr. Paul D. Kemmitt Prof. Paul Brennan Prof. Frank von Delft Dr. Laura Díaz Sáez Prof. Kilian Huber Prof. John Spencer 《ChemMedChem》2020,15(24):2513-2520
Combined photochemical arylation, “nuisance effect” (SNAr) reaction sequences have been employed in the design of small arrays for immediate deployment in medium-throughput X-ray protein–ligand structure determination. Reactions were deliberately allowed to run “out of control” in terms of selectivity; for example the ortho-arylation of 2-phenylpyridine gave five products resulting from mono- and bisarylations combined with SNAr processes. As a result, a number of crystallographic hits against NUDT7, a key peroxisomal CoA ester hydrolase, have been identified. 相似文献
4.
Alpidem, an imidazopyridine that acts at the gamma-aminobutyric acid/benzodiazepine receptor complex, has been reported to be an effective anxiolytic with a more favorable side effect profile than benzodiazepines. The effect of alpidem was investigated in an 8-week, open, clinical trial in 13 patients with panic disorder, with or without agoraphobia. Three patients were responders (much improved or very much improved), five patients were nonresponders, and five patients dropped out after less than 6 weeks of treatment. Significant improvement was seen in the sample as a whole for spontaneous panic attacks, phobic avoidance, and anticipatory anxiety. Most improvement occurred during the first 4 weeks of treatment, and responders had milder panic disorder at baseline. Adverse effects were generally mild. After 8 weeks of treatment, taper of medication over 2 weeks occurred without significant worsening of panic disorder symptoms. The efficacy of alpidem in the treatment of panic disorder remains uncertain and requires assessment in a controlled trial. 相似文献
5.
An intracellular endopeptidase was purified from cell-free extracts of Lactobacillus delbrueckii subsp. bulgaricus B14 by anion exchange chromatography on DEAE-Sepharose, hydroxyapatite chromatography, second anion exchange chromatography on Mono-Q, and metal-chelating affinity chromatography. The endopeptidase was a monomer with a molecular mass of approximately 70 kDa determined by SDS-PAGE and gel filtration. Various oligopeptides (e.g. Met-enkephalin, bradykinin) were hydrolysed by the endopeptidase. Exopeptidase activity and cleavage of dipeptides or tripeptides was not observed. The KM value for the cleavage of Metenkephalin was 1.2 mM. Temperature and pH optima were 47 °C and pH 7.7, respectively. The endopeptidase was inhibited by the classical agents for metal-dependent (EDTA) and serine (DFP) enzymes. Activity was increased by Co2+ and Mg2+, no effect was observed with Ca2+. After inhibition with EDTA, enzyme activity could be restored fully by Co2+. Activity was inhibited by Zn2+, Mn2+, Fe2+, Cu2+, Cd2+ and Hg2+. The N-terminal sequence of the endopeptidase was determined as: H2N-Val-Arg-Gly-Gly-Ser-Gly-Asp-Thr-Thr-Val-0H. 相似文献
6.
A unified theoretical method for the calculation of the radio capacity of multiple-access schemes such as FDMA (frequency-division multiple access), TDMA (time-division multiple access), CDMA (code-division multiple access) and SSMA (spread-spectrum multiple access) in noncellular and cellular mobile radio systems is presented for AWGN (additive white Gaussian noise) channels. The theoretical equivalence of all the considered multiple-access schemes is found. In a fading multipath environment, which is typical for mobile radio applications, there are significant differences between these multiple-access schemes. These differences are discussed in an illustrative manner revealing several advantages of CDMA and SSMA over FDMA and TDMA. Novel transmission and reception schemes called coherent multiple transmission and coherent multiple reception are briefly presented 相似文献
7.
Niklas Schlimm Mirko Novakovic Robert Spielmann Tobias Knierim 《Informatik-Spektrum》2007,48(6):251-258
Nicht-funktionale Anforderungen an ein Softwaresystem beschreiben Aspekte, die nicht direkt die Funktionalit?t, wie sie der
Benutzer sieht, betreffen. 相似文献
8.
In Europe, the challenge of the design of third-generation cellular mobile radio systems has been taken numerous research and development activities in this field have been started. The authors try to give an overview of the European activities in this field. The article is organized in two parts. In the first part, the requirements for third-generation cellular mobile radio systems such as Universal Mobile Telecommunications System (UMTS) and Future Public Land Mobile Telecommunications System (FPLMTS), which has been named International Mobile Telecommunications after the year 2000 (IMT-2000) are presented concisely. Also, the importance and challenge of the multiple access design for such third-generation cellular mobile radio systems is explained. In the second part, numerous European research activities with focus on the multiple access design for third-generation cellular mobile radio systems are reviewed 相似文献
9.
Tobias Unruh Jürgen Neuhaus Winfried Petry 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2007,580(3):1414-1422
The TOFTOF spectrometer is a multi-disc chopper time-of-flight spectrometer for cold neutrons at the research neutron source Heinz Maier-Leibnitz (FRM II). After five reactor cycles of routine operation the characteristics of the instrument are reported in this article. The spectrometer features an excellent signal to background ratio due to its remote position in the neutron guide hall, an elaborated shielding concept and an s-shaped curved primary neutron guide which acts i.a. as a neutron velocity filter. The spectrometer is fed with neutrons from the undermoderated cold neutron source of the FRM II leading to a total neutron flux of 1010n/cm2/s in the continuous white beam at the sample position distributed over a continuous and particularly broad wavelength spectrum. A high energy resolution is achieved by the use of high speed chopper discs made of carbon-fiber-reinforced plastic. In the combination of intensity, resolution and signal to background ratio the spectrometer offers new scientific prospects in the fields of inelastic and quasielastic neutron scattering. 相似文献
10.
Optimum spread spectrum signals for wideband channel sounding 总被引:1,自引:0,他引:1
Optimum periodic spread spectrum test signals suitable for wideband channel sounding are introduced. In contrast to conventional test signals based on m sequences, the proposed test signals with complex lowpass equivalents offer minimum crest factors in order to minimise the outband power spectral density at the output of a nonlinear transmitter amplifier.<> 相似文献