Endogenous and copper-induced lipid peroxidation and antioxidant activity of serum in hypercholesterolemic subjects

In order to observe the transport ability of peritoneum to small molecular substances, peritoneal equilibration test (PET) was performed in 52 CAPD patients. By analysing the relationship between peritoneal transport function and dialysis adequacy, we found the average urea KT/V and Cr were significantly lower in high and low transport groups (n = 6 and n = 2) than in high average and low average groups (n = 35 and n = 9). According to the results of PET, we adjusted the dialysis program of 11 patients and the dialysis adequacy was markedly improved. We concluded that PET was helpful for selecting and adjusting CAPD program, and discussed some questions which should be payed more attention in PET operation.     

Lipid peroxides, glutathione, gamma-glutamylcysteine synthetase and gamma-glutamyltranspeptidase activities in several tissues of rats following water-immersion stress

The effects of water-immersion restraint (WIR) stress on lipid peroxide, glutathione (GSH), glutathione peroxidase (GSH-Px), gamma-glutamylcysteine synthetase (gamma-GCS) and gamma-glutamyltranspeptidase (gamma-GT) activities in several tissues of rats were investigated. Hepatic and intestinal lipid peroxide levels were increased significantly in the WIR stress group. In both tissues, GSH levels were significantly decreased and gamma-GCS activity was significantly increased. In addition, gamma-GT activities remained unchanged in both tissues following WIR stress. However, lipid peroxide and GSH levels did not change in the stomach and brain in the WIR stress group compared to the control group. These results suggest that lipid peroxidation, but not the depression of GSH synthesis and/or the increase of GSH breakdown may be a factor in hepatic and intestinal GSH reduction following WIR stress.     

Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1)

BACKGROUND: Phosphorylation critically regulates the catalytic function of most members of the protein kinase superfamily. One such member, protein kinase C (PKC), contains two phosphorylation switches: a site on the activation loop that is phosphorylated by another kinase, and two autophosphorylation sites in the carboxyl terminus. For conventional PKC isozymes, the mature enzyme, which is present in the detergent-soluble fraction of cells, is quantitatively phosphorylated at the carboxy-terminal sites but only partially phosphorylated on the activation loop. RESULTS: This study identifies the recently discovered phosphoinositide-dependent kinase 1, PDK-1, as a regulator of the activation loop of conventional PKC isozymes. First, studies in vivo revealed that PDK-1 controls the amount of mature (carboxy-terminally phosphorylated) conventional PKC. More specifically, co-expression of the conventional PKC isoform PKC betaII with a catalytically inactive form of PDK-1 in COS-7 cells resulted in both the accumulation of non-phosphorylated PKC and a corresponding decrease in PKC activity. Second, studies in vitro using purified proteins established that PDK-1 specifically phosphorylates the activation loop of PKC alpha and betaII. The phosphorylation of the mature PKC enzyme did not modulate its basal activity or its maximal cofactor-dependent activity. Rather, the phosphorylation of non-phosphorylated enzyme by PDK-1 triggered carboxy-terminal phosphorylation of PKC, thus providing the first step in the generation of catalytically competent (mature) enzyme. CONCLUSIONS: We have shown that PDK-1 controls the phosphorylation of conventional PKC isozymes in vivo. Studies performed in vitro establish that PDK-1 directly phosphorylates PKC on the activation loop, thereby allowing carboxy-terminal phosphorylation of PKC. These data suggest that phosphorylation of the activation loop by PDK-1 provides the first step in the processing of conventional PKC isozymes by phosphorylation.     

Evaluating the Impact of User Characteristics and Different Layouts on an Interactive Visualization for Decision Making

There is increasing evidence that user characteristics can have a significant impact on visualization effectiveness, suggesting that visualizations could be designed to better fit each user's specific needs. Most studies to date, however, have looked at static visualizations. Studies considering interactive visualizations have only looked at a limited number of user characteristics, and consider either low‐level tasks (e.g., value retrieval), or high‐level tasks (in particular: discovery), but not both. This paper contributes to this line of work by looking at the impact of a large set of user characteristics on user performance with interactive visualizations, for both low and high‐level tasks. We focus on interactive visualizations that support decision making, exemplified by a visualization known as Value Charts. We include in the study two versions of ValueCharts that differ in terms of layout, to ascertain whether layout mediates the impact of individual differences and could be considered as a form of personalization. Our key findings are that (i) performance with low and high‐level tasks is affected by different user characteristics, and (ii) users with low visual working memory perform better with a horizontal layout. We discuss how these findings can inform the provision of personalized support to visualization processing.     

A Kharitonov‐like theorem for robust stability independent of delay of interval quasipolynomials

In this paper, a Kharitonov‐like theorem is proved for testing robust stability independent of delay of interval quasipolynomials, p(s)+∑eq_k(s), where p and q_k's are interval polynomials with uncertain coefficients. It is shown that the robust stability test of the quasipolynomial basically reduces to the stability test of a set of Kharitonov‐like vertex quasipolynomials, where stability is interpreted as stability independent of delay. As discovered in (IEEE Trans. Autom. Control 2008; 53 :1219–1234), the well‐known vertex‐type robust stability result reported in (IMA J. Math. Contr. Info. 1988; 5 :117–123) (See also (IEEE Trans. Circ. Syst. 1990; 37 (7):969–972; Proc. 34th IEEE Conf. Decision Contr., New Orleans, LA, December 1995; 392–394) does contain a flaw. An alternative approach is proposed in (IEEE Trans. Autom. Control 2008; 53 :1219–1234), and both frequency sweeping and vertex type robust stability tests are developed for quasipolynomials with polytopic coefficient uncertainties. Under a specific assumption, it is shown in (IEEE Trans. Autom. Control 2008; 53 :1219–1234) that robust stability independent of delay of an interval quasipolynomial can be reduced to stability independent of delay of a set of Kharitonov‐like vertex quasipolynomials. In this paper, we show that the assumption made in (IEEE Trans. Autom. Control 2008; 53 :1219–1234) is redundant, and the Kharitonov‐like result reported in (IEEE Trans. Autom. Control 2008; 53 :1219–1234) is true without any additional assumption, and can be applied to all quasipolynomials. The key idea used in (IEEE Trans. Autom. Control 2008; 53 :1219–1234) was the equivalence of Hurwitz stability and ?_‐o‐stability for interval polynomials with constant term never equal to zero. This simple observation implies that the well‐known Kharitonov theorem for Hurwitz stability can be applied for ?_‐o‐stability, provided that the constant term of the interval polynomial never vanishes. However, this line of approach is based on a specific assumption, which we call the CNF‐assumption. In this paper, we follow a different approach: First, robust ?_‐o‐stability problem is studied in a more general framework, including the cases where degree drop is allowed, and the constant term as well as other higher‐orders terms can vanish. Then, generalized Kharitonov‐like theorems are proved for ?_‐o‐stability, and inspired by the techniques used in (IEEE Trans. Autom. Control 2008; 53 :1219–1234), it is shown that robust stability independent of delay of an interval quasipolynomial can be reduced to stability independent of delay of a set of Kharitonov‐like vertex quasipolynomials, even if the assumption adopted in (IEEE Trans. Autom. Control 2008; 53 :1219–1234) is not satisfied. Copyright © 2009 John Wiley & Sons, Ltd.     

On the complexity of purely complex μ computation and relatedproblems in multidimensional systems

In this paper, the following robust control problems are shown to be NP-hard: given a purely complex uncertainty structure Δ, determine if: 1) μ_Δ(M)<1, for a given rational matrix M; 2) ∥M(·)∥_μ<1, for a given rational transfer matrix M(s); and 3) inf(Q∈H^∞)∥F(T,Q)∥_μ<1, for a given linear fractional transformation F(T,Q) with rational coefficients. In other words, purely complex μ computation, analysis, and synthesis problems are NP-hard. It is also shown that checking stability and computing the H^∞ norm of a multidimensional system, are NP-hard problems. Therefore, it is rather unlikely to find nonconservative polynomial time algorithms for solving the problems in complete generality     

Investigating the effect of production process of ball mill refiner on some physical quality parameters of compound chocolate: response surface methodology approach

Chocolate compound was produced using ball mill refiner, and the effect of agitator shaft speed and refining time on the physical quality parameters (particle size, colour and steady‐state rheology) of compound chocolate was determined using response surface methodology. The shaft speed and refining time range were selected between 40–60 r.p.m. and 10–30 min, respectively. Determination coefficient of the models established for particle size, Newtonian viscosity and colour parameters (brightness, chroma and hue angle) were found to be very close to unity. Increasing shaft speed and time induced a reduction in particle size and an increase in viscosity of the samples. Temperature sweep test was also performed, and the obtained data were successfully fitted to Arrhenius equation to calculate the corresponding parameters representing temperature dependency of the compounds. The results highlighted that the establishment of such models can provide essential information in terms of optimisation of production processes regarding usage purpose of the compound chocolate.     

Bioactive and Physicochemical Properties of Persimmon as Affected by Drying Methods

In this study, the effects of three different drying methods (freeze drying, oven drying, and vacuum oven drying) on bioactive (total phenolics, total flavonoids, condensed tannin and total hydrolyzable tannin contents, antiradical activity, and antidiabetic activity) and some physicochemical (dry matter, ash, water activity (a _w ), color, protein, hydroxymethylfurfural, glucose and fructose content) properties of persimmon fruit were investigated. Simplex lattice mixture design methodology was applied to determine the best solvent mixture for the extraction of phenolics from the samples. It was found that the mixture of acetone:water at the ratio of 50:50 % (v/v) was the best solvent mixture for the extraction. The persimmon powder sample obtained from freeze drying showed significantly (p <0.05) higher bioactivity values than oven- and vacuum-oven-dried samples. Antiradical activity changed significantly depending on the drying method employed and it was superior in freeze-dried samples than that of the other drying methods.