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Jos Torquato Q Tavares DBora Q Tavares Manoel A C Miranda 《Journal of the science of food and agriculture》1993,62(2):207-208
The homozygous inheritance of recessive alleles for lipoxygenase, -L1, -L2 and -L3 incorporated into a commercial soya bean seed line, IAC 8, was evaluated. The activity of each isozyme, determined by spectrophotometry demonstrated a very low activity for the mutant -L1 and respective progeny and a low activity for -L2, -L3 and their progenies. The methodology employed was shown to be a reliable and rapid method when evaluating lipoxygenase from crops. 相似文献
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L Di Ciero ML Oliva R Torquato P K?hler JK Weder J Camillo Novello CA Sampaio B Oliveira S Marangoni 《Canadian Metallurgical Quarterly》1998,17(8):827-834
Trypsin inhibitors of two varieties of Bauhinia variegata seeds have been isolated and characterized. Bauhinia variegata candida trypsin inhibitor (BvcTI) and B. variegata lilac trypsin inhibitor (BvlTI) are proteins with Mr of about 20,000 without free sulfhydryl groups. Amino acid analysis shows a high content of aspartic acid, glutamic acid, serine, and glycine, and a low content of histidine, tyrosine, methionine, and lysine in both inhibitors. Isoelectric focusing for both varieties detected three isoforms (pI 4.85, 5.00, and 5.15), which were resolved by HPLC procedure. The trypsin inhibitors show Ki values of 6.9 and 1.2 nM for BvcTI and BvlTI, respectively. The N-terminal sequences of the three trypsin inhibitor isoforms from both varieties of Bauhinia variegata and the complete amino acid sequence of B. variegata var. candida L. trypsin inhibitor isoform 3 (BvcTI-3) are presented. The sequences have been determined by automated Edman degradation of the reduced and carboxymethylated proteins of the peptides resulting from Staphylococcus aureus protease and trypsin digestion. BvcTI-3 is composed of 167 residues and has a calculated molecular mass of 18,529. Homology studies with other trypsin inhibitors show that BvcTI-3 belongs to the Kunitz family. The putative active site encompasses Arg (63)-Ile (64). 相似文献
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Elastic Properties and Structure of Interpenetrating Boron Carbide/Aluminum Multiphase Composites 总被引:1,自引:0,他引:1
Salvatore Torquato Christofer L. Y. Yeong Mark D. Rintoul David L. Milius Ilhan A. Aksay 《Journal of the American Ceramic Society》1999,82(5):1263-1268
We study the elastic moduli and structure of boron carbide/aluminum (B4 C/Al) multiphase composites using rigorous bounding and experimental characterization techniques. We demonstrate that rigorous bounds on the effective moduli are useful in that they can accurately predict (i) the effective elastic moduli, given the phase moduli and volume fractions, or (ii) the phase moduli (volume fractions), given the effective moduli and phase volume fractions (moduli). Using the best available rigorous bounds on the effective elastic moduli of multiphase composites involving volume-fraction information, we are able to predict the bulk and shear moduli of the Al4 BC phase, a reaction product that forms during heat treatment. These theoretical predictions are in very good agreement with recent experimental measurements of the moduli of the Al4 BC phase. Moreover, we evaluate more-refined bounds involving three-point structural correlation functions by extracting such information from an image of a sample of the B4 C/Al composite. Although experimental data for the effective moduli are unavailable for this sample, our predictions of the effective moduli based on three-point bounds should be quite accurate. 相似文献
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Priscila F. de Melo Daneysa L. Kalschne Rosana A. da Silva-Buzanello Joana S. Amaral Alex S. Torquato Marinês P. Corso Heloisa G. Falcão Eliane Colla Elza I. Ida Cristiane Canan 《International Journal of Food Science & Technology》2020,55(1):397-405
The aim of this study was to produce cereal bars (CB) added with tempeh flour (TF) and evaluate the nutritional composition, fatty acids profile, isoflavone content and sensory acceptance. Tempeh was produced from organic soya beans cotyledons fermented by Rhizophus oligosporus followed by freeze-drying and milling in order to obtain the TF. The addition of 10%, 15% and 20% TF to CB resulted in both protein and lipid content increase and a higher polyunsaturated/saturated fatty acids ratio. The content of isoflavone aglycones, the forms considered to have higher biological activity, was higher in TF than in soya bean, which was also reflected in the CB chemical composition. An acceptance test showed that CB added with TF (up to 15%) were sensory accepted. Overall results suggest that the addition of TF as an ingredient in cereal bars allows improving the chemical and functional characteristics of this type of ready-to-eat foods. 相似文献