Failed Neuroprotection of Combined Inhibition of L-Type and ASIC1a Calcium Channels with Nimodipine and Amiloride

Effective pharmacological neuroprotection is one of the most desired aims in modern medicine. We postulated that a combination of two clinically used drugs—nimodipine (L-Type voltage-gated calcium channel blocker) and amiloride (acid-sensing ion channel inhibitor)—might act synergistically in an experimental model of ischaemia, targeting the intracellular rise in calcium as a pathway in neuronal cell death. We used organotypic hippocampal slices of mice pups and a well-established regimen of oxygen-glucose deprivation (OGD) to assess a possible neuroprotective effect. Neither nimodipine (at 10 or 20 µM) alone or in combination with amiloride (at 100 µM) showed any amelioration. Dissolved at 2.0 Vol.% dimethyl-sulfoxide (DMSO), the combination of both components even increased cell damage (p = 0.0001), an effect not observed with amiloride alone. We conclude that neither amiloride nor nimodipine do offer neuroprotection in an in vitro ischaemia model. On a technical note, the use of DMSO should be carefully evaluated in neuroprotective experiments, since it possibly alters cell damage.     

Zur Verantwortung des Informatikers-Militärische Anwendungen der Informatik

Ohne Zusammenfassung     

A Multinomial Model of Event-Based Prospective Memory.

Prospective memory is remembering to perform an action in the future. The authors introduce the 1st formal model of event-based prospective memory, namely, a multinomial model that includes 2 separate parameters related to prospective memory processes. The 1st measures preparatory attentional processes, and the 2nd measures retrospective memory processes. The model was validated in 4 experiments. Manipulations of instructions to place importance on either the prospective memory task or the background task (Experiments 1 and 2) and manipulations of distinctiveness of prospective memory targets (Experiment 2) had expected effects on model parameters, as did a manipulation of the difficulty of prospective memory target encoding (Experiments 3 and 4). An alternative model was also evaluated. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Numerical simulation of ion-exchange in glass for integratedoptical components

A numerical model based on the finite-element method for solving the nonlinear diffusion equation of ion-exchange in glasses is presented. The model is applied to the calculation of the concentration profile for a Ag⁺-Na⁺ ion-exchanged channel waveguide. The calculated concentration profile is in good agreement with a measured refractive-index profile of a waveguide fabricated with the same parameters as used in the calculations     

Adequacy of a translation of the MMPI into American Sign Language for use with deaf individuals: Linguistic equivalency issues.

Translated the MMPI into American Sign Language (ASL) via the back-translation procedure and recorded it on videotape to determine the linguistic equivalency of a sign language translation of a psychological test for use with deaf individuals. The bilingual retest technique was conducted whereby both forms of the instrument were administered to 28 ASL-English bilingual deaf Ss. Due to the advent of the MMPI-2 during the conduct of this study, a new set of T scores was calculated from the present MMPI data to compare the effect of shifting to the MMPI-2 norms. The results demonstrate adequate linguistic equivalencies of the ASL MMPI items and underscore the potential utility and practicality of future ASL translations of psychological tests for use with deaf individuals. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Combustion of bulk density powder mixtures in a coflow of nitrogen gas: 3. The Ti-(Ti + 0.5C) layered system

Explored is the filtration combustion in Ti-(Ti + 0.5C) layered powder systems in a coflow of nitrogen gas. The presence/absence of nitrogen coflow through the layered system was found to drastically affect the character of wave propagation and structure/properties of product. The obtained data can be regarded as a basis for fabrication of new layered and composite materials in the mode of dynamic filtration combustion.      

Machining of reproducible flaws corresponding to fatigue cracks for fracture mechanics tests of components and welded joints

In connection with the analysis and the development of failure concepts in fracture mechanics for quasistatic loaded components and elastic-plastic behaviour of the material, tests are also carried out with welded and/or complex shaped specimens or structures. Thereby the difficulty arises of generating reproducible flaws in the form of fatigue precracks in definite positions in the components, respectively in the welded joint. It is reported exemplarily about experiments on different CT25 and CCT specimens and on a pressure vessel which contained a fatigue pre-crack, a 0.2 mm saw cut or notches with notch root radius ≤ ≥ 0.1 mm as flaws. The comparison of the results with regard to J-integral at initiation of stable crack, J_i, and J_R curves shows that under certain conditions the 0.2 mm saw cut (notch root radius ≤ ≤ 0.02 mm) is a useful alternative, if reproducible generation of a fatigue pre-crack will not be successful or too expensive. The tests were carried out on StE 460 and on a welded joint of this steel at 25 ± 2°C.     

Polycyclic Aromatic Hydrocarbons Activate the Aryl Hydrocarbon Receptor and the Constitutive Androstane Receptor to Regulate Xenobiotic Metabolism in Human Liver Cells

Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants produced by incomplete combustion of organic matter. They induce their own metabolism by upregulating xenobiotic-metabolizing enzymes such as cytochrome P450 monooxygenase 1A1 (CYP1A1) by activating the aryl hydrocarbon receptor (AHR). However, previous studies showed that individual PAHs may also interact with the constitutive androstane receptor (CAR). Here, we studied ten PAHs, different in carcinogenicity classification, for their potential to activate AHR- and CAR-dependent luciferase reporter genes in human liver cells. The majority of investigated PAHs activated AHR, while non-carcinogenic PAHs tended to activate CAR. We further characterized gene expression, protein abundancies and activities of the AHR targets CYP1A1 and 1A2, and the CAR target CYP2B6 in human HepaRG hepatoma cells. Enzyme induction patterns strongly resembled the profiles obtained at the receptor level, with AHR-activating PAHs inducing CYP1A1/1A2 and CAR-activating PAHs inducing CYP2B6. In summary, this study provides evidence that beside well-known activation of AHR, some PAHs also activate CAR, followed by subsequent expression of respective target genes. Furthermore, we found that an increased PAH ring number is associated with AHR activation as well as the induction of DNA double-strand breaks, whereas smaller PAHs activated CAR but showed no DNA-damaging potential.