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排序方式: 共有658条查询结果,搜索用时 16 毫秒
1.
Sebastian Roth Peter Stockinger Jakob Steff Simon Steimle Dr. Viktor Sautner Prof. Dr. Kai Tittmann Prof. Dr. Jürgen Pleiss Prof. Dr. Michael Müller 《Chembiochem : a European journal of chemical biology》2020,21(18):2615-2619
The family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs) comprises numerous biocatalysts capable of C=O or C=C reduction. The highly homologous noroxomaritidine reductase (NR) from Narcissus sp. aff. pseudonarcissus and Zt_SDR from Zephyranthes treatiae, however, are SDRs with an extended imine substrate scope. Comparison with a similar SDR from Asparagus officinalis (Ao_SDR) exhibiting keto-reducing activity, yet negligible imine-reducing capability, and mining the Short-Chain Dehydrogenase/Reductase Engineering Database indicated that NR and Zt_SDR possess a unique active-site composition among SDRs. Adapting the active site of Ao_SDR accordingly improved its imine-reducing capability. By applying the same strategy, an unrelated SDR from Methylobacterium sp. 77 (M77_SDR) with distinct keto-reducing activity was engineered into a promiscuous enzyme with imine-reducing activity, thereby confirming that the ability to reduce imines can be rationally introduced into members of the “classical” SDR enzyme family. Thus, members of the SDR family could be a promising starting point for protein approaches to generate new imine-reducing enzymes. 相似文献
2.
Viktor G. Khizhnyak Vitalii I. Korol' Alexei D. Kostenko 《Powder Metallurgy and Metal Ceramics》2003,42(11-12):644-648
Wear resistance measurements have been made with U8A steel bearing protective coatings based on the carbides of titanium, vanadium, and chromium or iron borides, for various forms of wear. The carbide and boride coatings raise the wear resistance considerably (by factors of 6-30 in accordance with the type of wear). Measurements have been made on the microhardness, microbrittleness, and adhesion of the coating to the substrate, which are effective characteristics that quite fully reflect the effects of the microhardness and state of stress, structure, and defectiveness on the wear resistance. 相似文献
3.
This article quantifies errors arising from various integration methods and from sampling density in the numerical estimation of colorimetric integrals, e.g., tristimulus values. Both data sampling density and various numerical integration method errors will be distinguished. A test is presented to quantify sampling interval requirements for accurate colorimetric calculations for various illuminants. © 1992 John Wiley & Sons, Inc. 相似文献
4.
We have designed Particle-in-Cell algorithms for emerging architectures. These algorithms share a common approach, using fine-grained tiles, but different implementations depending on the architecture. On the GPU, there were two different implementations, one with atomic operations and one with no data collisions, using CUDA C and Fortran. Speedups up to about 50 compared to a single core of the Intel i7 processor have been achieved. There was also an implementation for traditional multi-core processors using OpenMP which achieved high parallel efficiency. We believe that this approach should work for other emerging designs such as Intel Phi coprocessor from the Intel MIC architecture. 相似文献
5.
Petr Kocna Thomas Mothes Viktor Krchnák Premysl Fric 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1991,192(2):116-119
Summary The tendency to form a-turn in-gliadin was estimated using the B-cell determinant prediction program based on the Chou and Fasman probability of-turn formation. Six sequences possessing a high probability of-turn formation were found. A statistically high agreement was found between these six sequences and three areas in-gliadin with the occurrence of Pro-Ser-Gln-Gln sequence which has recently been considered responsible for toxicity in coeliac disease. By means of solid-phase synthesis seven peptides were obtained covering the above-mentioned regions. Their toxicity was tested using the fetal chick duodenum. The results support the suggestion that peptides containing the sequences Pro-Ser-Gln-Gln and Gln-Gln-Gln-Pro may be involved in the pathogenesis of coeliac disease.
Beziehung zwischen der Gliadin-Peptid-Struktur und ihr Einfluß auf den fetalen Kückendarm
Zusammenfassung Die Tendenz zur Bildung einer-Umwandlung im-Gliadin wurde bei Anwendung eines mathematischen Programms zur Vorhersage von B-Zelldeterminanten bestimmt, welches auf der Wahrscheinlichkeit der-Umwandlung nach Chou und Fasman basiert. Es wurden 6 Sequenzen gefunden, die eine hohe Wahrscheinlichkeit für die Bildung von-Umwandlungen aufwiesen. Zwischen diesen 6 Sequenzen und 3 Regionen im-Gliadin mit der Sequenz Pro-Ser-Gln-Gln, die kürzlich als verantwortlich für die Toxizität bei Cöliakie angesehen wurden, konnte eine statistisch gesicherte Beziehung gefunden werden. Mittels Festphasensynthese wurden 7 Peptide erhalten, die die oben genannten Regionen überdeckten. Ihre Toxizität wurde im fetalen Kückendarm getestet. Die Ergebnisse weisen darauf hin, daß Peptide, welche die Sequenz Pro-Ser-Gln-Gln und Gln-Gln-Gln-Pro enthalten, an der Pathogenese der Cöliakie beteiligt sein könnten.相似文献
6.
Marta Veningerová Viktor Prachar Ján Uhnák Jana Kovaiová 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1994,199(4):317-321
A method has been developed for the isolation, separation and determination of chlorinated phenols in total diet. The isolation takes place following acid hydrolysis of the conjugates and is performed by simultaneous extraction and distillation with water vapour and toluene. The isolated chlorophenols are removed by clean-up on a Florisil column using a mixture of 15% dichloromethane in toluene for elution. The separation and determination is carried out by capillary gas chromatography with electron capture detection. The recovery of the method ranged between 70.1±5 and 96.8±4.9% for the individual chlorophenols. The determination limits were 0.5 to 1.0 g·kg–1. The method was applied in a study aimed at estimating the chlorophenol burden in two population groups (children up to the age of 6 years and students up to the age of 18 years). In two succesive years, 80 total diet samples were analysed. Average contents of the individual chlorophenols were from 0.7 to 33.5 g·kg–1 and were higher for the dichlorophenol isomers. The calculated average daily intake ranged from 0.04 to 1.69 g·kg–1 body weight for the individual phenols.
Polychlorierte Phenole im Gesamtverzehr
Zusammenfassung Das hier beschriebene Verfahren dient zur Isolierung, Trennung und Bestimmung von chlorierten Phenolen im Gesamtverzehr. Dieser wurde sauer hydrolysiert, die Phenole wurden durch eine simultane Wasserdampfdestillation und ein Extraktionsverfahren mit Toluol isoliert. Beim anschließenden Derivatisierungsverfahren mit Pentafluorobenzylbromid entstanden auch Störungssubstanzen, sie wurden mittels Säulenchromatographie an Florisil abgetrennt. Die chromatographische Trennung und Bestimmung erfolgte unter Anwendung der Kapillargaschromatographie mit ECD. Die Wiederfindungsrate variierte zwischen 70.1±5 und 96.8±4.9% bei Nachweisgrenzen von 0.5 bis 1.0 g·kg–1. Das Verfahren wurde zur Ermittlung der Exposition bei Kindern bis zu 6 Jahren und Jungen bis zu 18 Jahren verwendet. Im Laufe von 2 Jahren wurden 80 Proben des Gesamtverzehrs aus Kindereinrichtungen analysiert. Der Durchschnittsinhalt schwankte von 0.7 bis 33.5 g·kg–1 mit Überwiegen von Dichlorophenolen. Die aus den bekannten Inhalten ermittelte Tagesaufnahme variiert zwischen 0.04 und 1.69 g·kg–1 Körpermasse und Tag.相似文献
7.
Christian Wolf Martin Prechtl René Bauer Michael Dinkel Fabian Beck Leopold Franz Viktor Neumeyer 《真空研究与实践》2023,35(1):35-39
To mark our anniversary, we are presenting articles that have been particularly appreciated by readers of our online edition once again in print. Here: 3D-Printing for High Vacuum Applications 3D printing technology has made the leap from a home-based private practice to industrial manufacturing. Due to the increasing reliability of printers and increasing material diversity, especially in the metal sector, double-digit percentage growth rates are possible in the coming years. This thesis deals with the manufacture of parts made by 3D printing for high vacuum application. Different components are printed and examined for their vacuum compatibility. As shown furthermore, conventionally made standard components can be vacuum sealed to printed parts, which enables cost-effective production of more complex components, such as e.g. a vacuum chamber allows. In addition, functional components can already be realized in the manufacturing process. The integration of a system of flow channels directly into the wall of a chamber is just one example. Thus, such a chamber can be heated during evacuation and effectively cool in later operation. 相似文献
8.
9.
Yurii G. Tkachenko Viktor F. Britun Emmanuil V. Prilutskii Dzhemma Z. Yurchenko Galina A. Bovkun 《Powder Metallurgy and Metal Ceramics》2005,44(3-4):196-201
We have investigated how the composition, grain morphology, and method of preparing the starting mixture affect the processes that form the structure and phase composition of B4C - SiC composites during hot pressing. We found that, depending on the composition of the initial powder mixtures, which is responsible for different mechanisms of consolidation of ceramic materials during hot pressing, the grain size of the main B4C phase and its defect content as well as the nature of the SiC phase distribution within the material differ significantly. When B4C - SiC composites with a low SiC content are made from initial B4C - B4Si - B - C powder mixtures those composites have a high cracking resistance because of their fine grain structure.__________Translated from Poroshkovaya Metallurgiya, Nos. 3–4(442), pp. 112–119, March–April, 2005. 相似文献
10.
Nam Ma Yinglong Xia Viktor K. Prasanna 《International journal of parallel programming》2014,42(1):219-237
We investigate data parallel techniques for belief propagation in acyclic factor graphs on multi-core systems. Belief propagation is a key inference algorithm in factor graph, a probabilistic graphical model that has found applications in many domains. In this paper, we explore data parallelism for basic operations over the potential tables in belief propagation. Data parallel techniques for these table operations are developed for shared memory platforms. We then propose a complete belief propagation algorithm using these table operations to perform exact inference in factor graphs. The proposed algorithms are implemented on state-of-the-art multi-socket multi-core systems with additional NUMA-aware optimizations. Our proposed algorithms exhibit good scalability using a representative set of factor graphs. On a four-socket Intel Westmere-EX system with 40 cores, we achieve 39.5 $\times $ speedup for the table operations and 39 $\times $ speedup for the complete algorithm using factor graphs with large potential tables. 相似文献