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Iliescu Florina Silvia Teo Jeremy Choon Meng Vrtacnik Danilo Taylor Hayden Iliescu Ciprian 《Microsystem Technologies》2018,24(7):2905-2912
Microsystem Technologies - Cell transplantation traditionally employs needles to inject donor cells into tissues to treat certain diseases. However, it is difficult for the current method to... 相似文献
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Maja Tomic Eda Vrtacnik Bokal Martin Stimpfel 《International journal of molecular sciences》2022,23(7)
This review focuses on recent findings in the preimplantation genetic testing (PGT) of embryos. Different preimplantation genetic tests are presented along with different genetic materials and their analysis. Original material concerning preimplantation genetic testing for aneuploidy (PGT-A) was sourced by searching the PubMed and ScienceDirect databases in October and November 2021. The searches comprised keywords such as ‘preimplantation’, ‘cfDNA’; ‘miRNA’, ‘PGT-A’, ‘niPGT-A’, ‘aneuploidy’, ‘mosaicism’, ‘blastocyst biopsy’, ‘blastocentesis’, ‘blastocoel fluid’, ‘NGS’, ‘FISH’, and ‘aCGH’. Non-invasive PGT-A (niPGT-A) is a novel approach to the genetic analysis of embryos. The premise is that the genetic material in the spent embryo culture media (SECM) corresponds to the genetic material in the embryo cells. The limitations of niPGT-A are a lower quantity and lesser quality of the cell-free genetic material, and its unknown origin. The concordance rate varies when compared to invasive PGT-A. Some authors have also hypothesized that mosaicism and aneuploid cells are preferentially excluded from the embryo during early development. Cell-free genetic material is readily available in the spent embryo culture media, which provides an easier, more economic, and safer extraction of genetic material for analysis. The sampling of the SECM and DNA extraction and amplification must be optimized. The origin of the cell-free media, the percentage of apoptotic events, and the levels of DNA contamination are currently unknown; these topics need to be further investigated. 相似文献
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Pokorny B Al Sayegh-Petkovsek S Ribaric-Lasnik C Vrtacnik J Doganoc DZ Adamic M 《The Science of the total environment》2004,324(1-3):223-234
In nature, animals have to cope with the fluctuating bioavailable metal pool in their habitat, which results in a seasonal variability of heavy metal levels in the animal body. Indeed, a pronounced summer-autumnal peak of heavy metals in roe deer (Capreolus capreolus L.) kidney was recently found in Slovenia. Considering the well-known hyperaccumulative ability of fungi, their ingestion was hypothesised to be one of the main reasons for the peak. Although fungi as a group are known to be a seasonally important food source for roe deer, data on their composition in the nutrition of the species have been lacking. To ascertain the importance of fungi ingestion on heavy metal intake in roe deer, we simultaneously studied fungal spores (by microscopic determination) and heavy metal levels (by inductively coupled plasma mass spectrometry and atomic absorption spectrometry) in roe deer faeces, collected in the period July-November 2001 at Veliki Vrh, the Salek Valley, Slovenia. Irrespective of species, fungal spores were present in 89% of faeces; the following genera were found to be consumed by roe deer: Lycoperdon, Calvatia, Hypholoma, Coprinus, Russula, Elaphomyces, Xerocomus, Enteloma, Amanita, Cortinarius, Agaricus, Inocybe, Boletus, Macrolepiota, Suillus and Pluteus. While the importance of fungi ingestion on the seasonal variability of other metals is less clear, it doubtless influences Hg intake in roe deer, which is confirmed by: (a) the high frequency of fungi in roe deer nutrition; (b) their hyperaccumulative ability; (c) the temporal distribution of Hg in roe deer faeces; (d) differences among three classes of faeces established on the basis of the frequency of spores present; (e) the correlation between the number of fungal genera present and Hg levels in faeces. Therefore, the influence of fungi ingestion has to be taken into consideration in assessing the hazard due to the accumulation of mercury along the food-chain. 相似文献
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