Squamous cell carcinoma of the penis. III. Treatment of regional lymph nodes

We analyzed the management of regional lymph nodes in 110 patients with squamous cell carcinoma of the penis treated at the Netherlands Cancer Institute between 1956 and 1989 with curative intent. Of 66 patients who presented with unsuspected nodes 57 were placed on a surveillance program, while lymph node dissection was performed in 5 (with adjuvant external radiation therapy in 1) and 4 were treated with external radiation therapy only. The management of 40 patients with clinically suspected nodes included surveillance in 5, lymph node dissection in 27 (with adjuvant radiotherapy in 11), biopsy in 4 and external radiation therapy in 4. Postoperative radiotherapy had been given if more than 2 nodes were involved or when extracapsular growth was observed. Overall, 25 patients had a regional recurrence, 5 of whom could be cured subsequently. All regional recurrences developed within 2 years after primary treatment. Analysis showed 100% survival in histologically proved node negative patients (stage pN0). The success of lymph node dissection was related to the extent of the metastatic spread and to the number of involved nodes. Patients with 1 positive node and unilateral inguinal involvement showed a statistically significant survival advantage compared to patients with more extensive spread. Considering the indications for node dissection we found a clear relationship among T category, grade and the probability of lymph node invasion. Patients with stage T1 tumors and stage T2, grades 1 and 2 tumors presented significantly less often with lymphatic invasion than those with other categories of disease and were less likely to have a regional recurrence after treatment of the primary tumor only. In these categories we recommend surveillance of the regional lymph nodes in patients who present with unsuspected nodes. However, patients with stage T2 grade 3, stage T3 and operable stage T4 tumors should undergo an immediate inguinal node dissection because of the high probability of clinically occult lymph node invasion (in our material more than 50%). With respect to the extent of the node dissection, we found that the likelihood of spread to the contralateral and/or pelvic regions was related to the number of invaded nodes in the inguinal region. We recommend contralateral node dissection and unilateral pelvic node dissection when 2 or more positive nodes are found in the dissected groin specimen. Primary pelvic node dissection should be performed in patients who present initially with cytologically or biopsy proved positive inguinal nodes.(ABSTRACT TRUNCATED AT 400 WORDS)     

Synthesis from component libraries

Synthesis is the automated construction of a system from its specification. In the classical temporal synthesis algorithms, it is always assumed the system is “constructed from scratch” rather than “composed” from reusable components. This, of course, rarely happens in real life. In real life, almost every non-trivial commercial system, either in hardware or in software system, relies heavily on using libraries of reusable components. Furthermore, other contexts, such as web-service orchestration, can be modeled as synthesis of a system from a library of components. In this work, we define and study the problem of LTL synthesis from libraries of reusable components. We define two notions of composition: data-flow composition, for which we prove the problem is undecidable, and control-flow composition, for which we prove the problem is 2EXPTIME-complete. As a side benefit, we derive an explicit characterization of the information needed by the synthesizer on the underlying components. This characterization can be used as a specification formalism between component providers and integrators.     

Vector Space Semantics: A Model-Theoretic Analysis of Locative Prepositions

This paper introduces a compositional semantics of locativeprepositional phrases which is based on a vector space ontology.Model-theoretic properties of prepositions like monotonicity andconservativity are defined in this system in a straightforward way.These notions are shown to describe central inferences with spatialexpressions and to account for the grammaticality of prepositionmodification. Model-theoretic constraints on the set of possibleprepositions in natural language are specified, similar to the semanticuniversals of Generalized Quantifier Theory.     

Phenylacetyl and Phenylpropionyl Amino Acids: Their Inhibitory Effect on Glutamine Synthetase and Their Resistance to Acylase. I

The preparation of a number of phenylacetyl and 3-phenylpropionyl derivatives of amino acids is described. Derivatives containing more than one aromatic group markedly inhibit ovine brain glutamine synthetase. All phenylacetyl and phenylpropionyl derivatives tested were resistant to hog kidney acylase I.     

Cold stress-induced neuroinvasiveness of attenuated arboviruses is not solely mediated by corticosterone

In previous studies we have shown that various stress paradigms can induce the penetration of noninvasive, attenuated viruses into the central nervous system (CNS). Since glucocorticoids levels are elevated during stress, we compared the effect of cold stress and corticosterone (CS) injection on neuroinvasiveness of a non-invasive encephalitic virus, WN-25 (West Nile). Exposure of inoculated mice to cold stress or CS resulted in high viremia and a marked increase in mortality when compared to control untreated mice. Exposure of WN-25 inoculated mice to cold treatment or CS injection led to high blood virus levels as compared to nontreated mice (3.2 and 3.1 vs > 1 log 10 PFU/ml). Cold stress or CS (5000 ng/mouse) treatment caused a mortality rate of 70% and 50% of the WN-25 inoculated mice respectively. No mortality was recorded in control inoculated groups (p < 0.05). Passive transfer serum from uninfected cold stressed mice to WN-25 inoculated nonstressed mice, resulted in similar mortality. The levels of CS in passive transferred serum from cold stressed animals was 500 ng/ml, only 2% (100 vs. 5000 ng) of the CS dose required to obtain a similar effect on viral penetration and mortality when CS was injected directly. Therefore, we concluded that CS was not the sole factor responsible for the cold stress effect on the viral infection outcome.     

Characterization of heparin and heparan sulfate domains binding to the long splice variant of platelet-derived growth factor A chain

Platelet-derived growth factors (PDGFs) are homo- or heterodimers of two related polypeptides, known as A and B chains. The A chain exists as two splice variants due to the alternative usage of exons 6 (PDGF-AL, longer) and 7 (PDGF-AS, shorter). Exon 6 encodes an 18-amino acid sequence rich in basic amino acid residues, which has been implicated as a cell retention signal. Several lines of evidence indicate that the retention is due to binding of PDGF-AL to glycosaminoglycans, especially to heparan sulfate. We have analyzed the saccharide domains of smooth muscle cell-derived heparan sulfate involved in this interaction. Furthermore, we have employed selectively modified heparin oligosaccharides to elucidate the dependence of the binding on different sulfate groups and on fragment length. The shortest PDGF-AL binding domain consists of 6-8 monosaccharide units. Studies using selectively desulfated heparins and heparin fragments suggest that N-, 2-O-, and 6-O-sulfate groups all contribute to the interaction. Structural comparison of heparan sulfate oligosaccharides separated by affinity chromatography on immobilized PDGF-AL showed that the bound pool was enriched in -IdceA(2-OSO3)-GlcNSO3(6-OSO3)- disaccharide units. Furthermore, analogous separation of a partially O-desulfated heparin decamer preparation, using a highly selective nitrocellulose filter-trapping system, yielded a PDGF-AL-bound fraction in which more than half of the disaccharide units had the structure -IdceA(2-OSO3)-GlcNSO3(6-OSO3)-. Our results suggest that the interaction between PDGF-AL and heparin/heparan sulfate is mediated via N-sulfated saccharide domains containing both 2-O- and 6-O-sulfate groups.     

Influence of temperature on magnetic properties of ion-beam sputter-deposited perpendicular magnetic media

Co-Cr perpendicular magnetic media deposited by ion-beam sputter methods show magnetic properties similar to those obtained by radio frequency (RF) sputtering. Coercivity, ratio of perpendicular to parallel squareness, and X-ray orientation are strikingly improved when the substrate is heated. Independent control of substrate temperature makes ion-beam sputter deposition an attractive candidate for deposition of perpendicular magnetic films.