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A reusable capacitive immunosensor with a novel immobilization procedure based on 1,6-hexanedithiol and nano-Au self-assembled layers 总被引:2,自引:0,他引:2
A novel immobilization procedure of antibody proteins for capacitive immunosensing, based on thiolor sulfur compound (1,6-hexanedithiol, HDT) and colloid Au layers is proposed. The insulating organic monolayer film was first formed by the spontaneous assembly of HDT from solution onto gold. When these thiol-rich surfaces are exposed to Au colloid, the sulfurs form strong bonds to gold nanoparticles, anchoring the clusters to the electrode substrate. After the assembly of gold nanoparticles layer, the original formed organic thiols surface was restored, and a new nano-Au surface was obtained. Thus, the antibody could be immobilized through electrostatic adsorption between nano-Au and the antibody proteins. After use, the formed immunocomplex layer can be rinsed out, via a saline solution with extreme pH. Therefore, the immunosensor can be regenerated repeatedly, highlighting a clear advantage of this new approach with respect to classical immunoassays employing covalent immobilization. 相似文献
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本文记述了大理崇圣寺三塔景区详细规划过程申的构思。以三塔历史的回顾为出发点论述了今日的规划指导思想,分析了现状存在的问题。在规划布局中尽量以塔为中心,旨在提高塔的历史、科学与艺术价值。规划中运应多种手段来组织空间,以丰富全区的景观。最后对三塔景区的远期规划设思作了概述。 相似文献
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Yanyu Liu Ruichao Hou Dongming Zhou Rencan Nie Zhaisheng Ding Yanbu Guo Li Zhao 《International journal of imaging systems and technology》2021,31(1):391-411
Masses of research decompose the image into different levels of feature maps, but the structures and edges may not appropriately separated. This may cause the loss of image detail in the fusion process. Therefore, we design a robust method for multimodal medical image fusion using spectral total variation transform (STVT). In our method, the source images are first decomposed into a series of texture signatures (referred to as deviation components) and base components via STVT algorithm. Then combine the local structural patch measurement (LSPM) to fuse the deviation components, and the base components are merged using a spatial frequency (SF) dual‐channel spiking cortical model (SF‐DCSCM), in which the SF of base components are regarded as stimulus to activate DCSCM. Finally, the final image is reconstructed by the inverse STVT with the restored images together. Experimental results suggest that proposed scheme achieves promising results, and more competitiveness against some state‐of‐the‐art methods. 相似文献
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为提高碾茶品质,考察遮荫方式(直接覆盖、高棚覆盖和混合覆盖)和遮阴时间(6、10、15、20 d)、杀青机蒸汽流量(60、80、100、120、140 kg/h)和烘干温度(210、220、230、250℃)等关键工艺参数对碾茶感官品质、理化成分的影响,并研究碾茶加工过程中理化成分的变化。结果表明:遮荫方式以混合覆盖加工的碾茶综合品质最佳;直接覆盖6 d、高棚覆盖15 d时加工的碾茶品质均较好。杀青蒸汽流量为120 kg/h时,加工的碾茶酚氨比最小,茶氨酸含量最高,能较好保留茶叶营养成分;烘干温度220℃干燥的碾茶感官品质整体较优,叶绿素和氨基酸含量最高。相关性分析表明,碾茶感官品质总得分与叶绿素a(r=0.741,P<0.01)、叶绿素b(r=0.767,P<0.01)和总叶绿素(r=0.754,P<0.01)含量呈极显著正相关,而茶汤浓度与其色差L*值呈极显著负相关(r=-0.817,P<0.01)。 相似文献
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