重组内切几丁质酶的纯化及其催化壳聚糖降解的研究

目的:研究重组内切几丁质酶的分离纯化及其催化壳聚糖制备壳寡糖的反应条件优化。方法:重组菌发酵液经PEG20000浓缩和DEAE-Sepharose FF离子交换层析后,测定总蛋白含量和内切几丁质酶活力。用纯化的内切几丁质酶催化壳聚糖制备壳寡糖,探讨其最佳的反应条件。结果:纯化的内切几丁质酶比活力41.34U/mg,纯化倍数2.49,酶总回收率89.63%。内切几丁质酶催化壳聚糖降解制备壳寡糖的最优化反应条件是:壳聚糖质量分数4%,p H7.0,温度30℃,时间12 h。结论:本研究结果为内切几丁质酶和壳寡糖的产业化应用奠定了良好基础。     

浙江省台州市新农村公共文化设施中的宗教建筑调查研究

以浙江省台州市新农村宗教建筑现状问卷调查为基础,分析了台州市村落在新农村规划建设过程中宗教建筑现状、宗教文化活动的开展情况等,总结出台州市新农村建设过程中宗教及其建筑发展特点,并对新农村规划建设过程中如何保护宗教建筑,促进宗教建筑的发展提出建议。     

Study on synthesis and electrochemical properties of novel ferrocene-based compounds and their applications in anion recognition

Two novel ferrocene-based compounds glycidyl ester of ferrocene carboxylate (GEFC) and 1,3-diferrocenecarboxylic acid diacylglycerol (DFCDG) were synthesized and characterized by ¹H NMR and mass spectrum. The influence of some factors, namely, solvent type, potential scan rate, electrolyte concentration and [Fe] unit concentration, on the electrochemical behaviors of two novel ferrocene-based compounds was investigated, and the mechanism of the electrode processes was studied. Finally, two compounds were applied to sense the H₂PO₄⁻ by cyclic voltammetry (CV) method and it was found that the DFCDG with a symmetric structure which was synthesized through ring opening reaction of ferrocene carboxylate and GEFC showed stronger interaction with the H₂PO₄⁻ than GEFC.     

Highly efficient subnanometer Ru-based catalyst for ammonia synthesis via an associative mechanism

The industrial manufacture of ammonia (NH₃) using Fe-based catalyst works under rigorous conditions. For the goal of carbon-neutrality, it is highly desired to develop advanced catalyst for NH₃ synthesis at mild conditions to reduce energy consumption and CO₂ emissions. However, the main challenge of NH₃ synthesis at mild conditions lies in the dissociation of steady NN triple bond. In this work, we report the design of subnanometer Ru clusters (0.8 nm) anchored on the hollow N-doped carbon spheres catalyst (Ru-SNCs), which effectively promotes the NH₃ synthesis at mild conditions via an associative route. The NH₃ synthesis rate over Ru-SNCs (0.49% (mass) Ru) reaches up to 11.7 mmol NH₃·(g cat)^-1·h^-1 at 400 ℃ and 3 MPa, which is superior to that of 8.3 mmol NH₃·(g cat)^-1·h^-1 over Ru nanoparticle catalyst (1.20% (mass) Ru). Various characterizations show that the N₂H₄ species are the main intermediates for NH₃ synthesis on Ru-SNCs catalyst. It demonstrates that Ru-SNCs catalyst can follow an associative route for N₂ activation, which circumvents the direct dissociation of N₂ and results in highly efficient NH₃ synthesis at mild conditions.     

Seamless Mipmap Filtering for Dual Paraboloid Maps

Dual paraboloid mapping is an approach for environment mapping. Its major advantage is its fast map generation speed. For graphics applications, when filtering is needed, the filtering tool would naturally be mipmapping. However, directly applying mipmapping to dual paraboloid mapping would give us three problems. They are the discontinuity across the dual paraboloid map boundary, the non‐uniform sampling problem and the depth testing issue. We propose three approaches to solve these problems. Our approaches are based on some closed form equations derived via theoretical analysis. Using these equations, we modify the coordinates involved during the rendering process. In other words, these problems are handled just by using dual paraboloid maps and mipmaps differently, instead of fundamentally altering their data structures. Consequently, we are fixing the problems without damaging the map generation speed advantage. Applying all three approaches, we improve the rendering quality of dual paraboloid map mipmaps to a level equivalent to that of cubemap mipmaps, while preserving its fast map generation speed advantage. This gives dual paraboloid map mipmaps the potential to be a better choice than cubemap mipmaps for the devices with less computational power. The effectiveness and the efficiency of the proposed approaches are demonstrated using a glossy reflection application and an omnidirectional soft shadow generation application.     

水力旋流分离制备不同应用特性糯米粉的研究

本研究以糯米为原料,经蛋白酶适度水解后采用水力旋流的方式分离,通过优化旋流分离的工艺参数,获得三种不同的糯米粉样品。样品1、样品2和样品3的蛋白含量分别为3.87%、0.46%和9.70%。试验测定糯米粉的透明度、溶解度、特征粘度和微观结构特性,结果表明:三种不同糯米粉样品外观聚集状态存在差异,随着蛋白含量的增加,可观察到蛋白包裹淀粉聚集体的存在,此时的糯米粉透明度和溶解度显著降低,抵抗加工的能力提高,崩解值降低,特征粘度值间存在显著(p<0.05)差异性。本研究降低了酶法制备糯米淀粉的实际操作难度,同时为食品工业提供不同应用特性的糯米粉产品,促进了稻米深加工行业的发展。     

一种新型模具扁钢生产工艺

考虑到目前模具扁钢市场的现状,借鉴国内外现有模具扁钢生产线的工艺路线及模具扁钢本身的性能特点,根据拟建工程中原料供应特点、产品大纲、产品质量和工程投资的要求,设计了一条新型模具扁钢生产线。介绍了这种新型模具钢扁钢生产线的工艺路线及主要设备参数,并分析了设计此工艺路线的思路及该工艺路线的优特点。说明了这是一条节约投资、产品质量可靠、物流顺畅的模具扁钢生产线,给现实生产、工程改造和将来的工程设计提供参考。     

群体感应抑制剂调控食源性微生物生物膜形成的研究进展

食源性微生物的生物膜是附着在固体表面上形成的具有空间组织的群落,因其能够附着在食品工业环境中的生物或非生物表面,且对消毒剂和抗菌剂能产生抗药性,通常难以控制,被认为是造成设备损坏、能源成本增加、食物变质和引起食源性疾病的原因之一,给食品工业带来了巨大的挑战。研究发现群体感应在生物膜的形成中起至关重要的作用,可以通过阻断群体感应系统来控制生物膜的形成。因此,群体感应抑制剂可以作为控制生物膜形成的新策略,在食品工业中对控制生物膜的形成具有巨大潜力。本文综述了生物膜的形成、群体感应系统及其对生物膜的调控作用,介绍了群体感应抑制剂的调控机制及其分类,为通过群体感应抑制剂调控生物被膜的形成提供研究思路。