Purification of the cardiac sarcoplasmic reticulum membrane protein phospholamban from recombinant Escherichia coli

Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification.     

Drug therapy of hemiballism. Review of the literature and personal observations

Hemiballism is a rare hyperkinetic disorder which is characterized by irregular vigorous rotatory movements of the limbs of one side of the body. The disabling and exhausting movements with their high risk of severe injuries require an acute drug treatment. However, there are no common guidelines for the treatment of this disorder. Therefore we reviewed the available literature to establish a concept for a rational symptomatic drug treatment. Furthermore, own observations in 6 patients with hemiballism of different origins are reported.     

Inotropic effects of glyceryl trinitrate and spontaneous NO donors in the dog heart

Facial fractures may portend intracranial and skullbase injury and may lead to rapid compromise of the airway. Primary care physicians may provide emergency care for patients who have sustained facial trauma. After immediate resuscitation and stabilization, management of facial fractures requires knowledge of the anatomy, rapid treatment methods and identification of potential associated injuries for each type of facial fracture. Differentiation between the life-threatening aspects of these injuries and the less urgent, but more apparent, facial injuries will lessen the risk of complications such as bleeding, meningitis and asphyxia. Knowledge of the anatomy of the facial skeleton and of the potential injuries associated with each of the various types of facial fractures will facilitate effective management decisions. Consultation should be sought when functional features are involved or when the injury threatens to produce future cosmetic anomalies.     

High-temperature creep and structure investigation of nearly stoichiometric Fe3Si

Single crystals of nearly stoichiometric Fe₃Si were creep-deformed at temperatureT = 450 to 850° C and applied stress=40 to 250 MPa. While the temperature dependence of the steady-state creep rate of crystals with less than 25 at% Si can be described by an exponential function exp (–H _exp/kT), the Fe-26 at% Si samples show an exponential dependence only below 500° C and above 600° C. At intermediate temperatures the dependence is weak. It is suggested that in this intermediate range two phases exist. The experimental results are consistent with the assumption that the phase boundaries do not hinder dislocation movement, and that the disocation velocity in the two phases is different.     

Nature-Inspired Meta-Heuristics on Modern GPUs: State of the Art and Brief Survey of Selected Algorithms

Graphic processing units (GPUs) emerged recently as an exciting new hardware environment for a truly parallel implementation and execution of Nature and Bio-inspired Algorithms with excellent price-to-power ratio. In contrast to common multicore CPUs that contain up to tens of independent cores, the GPUs represent a massively parallel single-instruction multiple-data devices that can nowadays reach peak performance of hundreds and thousands of giga floating-point operations per second. Nature and Bio-inspired Algorithms implement parallel optimization strategies in which a single candidate solution, a group of candidate solutions (population), or multiple populations seek for optimal solution or set of solutions of given problem. Genetic algorithms (GA) constitute a family of traditional and very well-known nature-inspired populational meta-heuristic algorithms that have proved its usefulness on a plethora of tasks through the years. Differential evolution (DE) is another efficient populational meta-heuristic algorithm for real-parameter optimization. Particle swarm optimization (PSO) can be seen as nature-inspired multiagent method in which the interaction of simple independent agents yields intelligent collective behavior. Simulated annealing (SA) is global optimization algorithm which combines statistical mechanics and combinatorial optimization with inspiration in metallurgy. This survey provides a brief overview of the latest state-of-the-art research on the design, implementation, and applications of parallel GA, DE, PSO, and SA-based methods on the GPUs.     

Polymeric absorbent for water sorption based on chemically crosslinked poly (acrylamide/2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt) hydrogels

Summary Swelling equilibrium of polyelectrolyte copolymer gels containing of acrylamide (AAm) and 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS) have been studied as a function of copolymer composition. AAm/AMPS hydrogels were prepared by free radical solution polymerization in aqueous solution of AAm with AMPS as anionic comonomer and two multifunctional crosslinkers such as ethylene glycol dimethacrylate (EGDMA) and trimethylolpropane triacrylate (TMPTA). Swelling experiments were performed in water at 25 °C, gravimetrically. The influence of AMPS content in hydrogels was examined. Swelling of AAm/AMPS hydrogels was increased up to 1018% (for containing 2% AMPS and crosslinked by EGDMA) 15246% (for containing 8% AMPS and crosslinked by TMPTA), while AAm hydrogels swelled up to 804% (crosslinked by TMPTA)–770% (crosslinked by EGDMA). The values of equilibrium water content of the hydrogels are 0.8851–0.9935. Diffusion behavior was investigated. Water diffusion into hydrogels was found to be non-Fickian in character.     

Synthetic polymeric absorbent for dye based on chemically crosslinked acrylamide/mesaconic acid hydrogels

Adsorption properties of copolymers of acrylamide and mesaconic acid (CAME) in aqueous Basic Blue 12 (Nile blue chloride) solution have been investigated. Chemically crosslinked CAME hydrogels with various compositions were prepared from ternary mixtures of acrylamide (A), mesaconic(ME) acid, and water by free radical polymerization in aqueous solution, using a multifunctional crosslinker such as ethylene glycol dimethacrylate (EGDMA). Dynamic swelling tests in water was applied to the hydrogels. Weight swelling ratio (S) values have been calculated. Sorption of Basic Blue 12 (BB 12) onto CAME hydrogels was studied by batch sorption technique at 25°C. In the experiments of the sorption, L type sorption in the Giles classification system was found. Some binding parameters such as initial binding constant (K_i), equilibrium constant (K), monolayer coverage (n), site‐size (u), and maximum fractional occupancy (Ô) for CAME hydrogels‐BB 12 binding system were calculated by using Klotz, Scatchard, and Langmuir linearization methods. Finally, the amount of sorbed BB 12 per gram of dry hydrogel (q) was calculated to be 2.28 × 10^?6–7.91× 10^?6 mol BB 12 per gram for hydrogels. Sorption % was changed range 16.09–58.86%. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 101: 405–413, 2006     

Support Vector machine and duration-aware conditional random field for identification of spatio-temporal activity patterns by combined indoor positioning and heart rate sensors

Tracking the spatio-temporal activity is highly relevant for domains like security, health, and quality management. Since animal welfare became a topic in politics and legislation locomotion patterns of livestock have received increasing interest. In contrast to the monitoring of pedestrians cattle activity tracking poses special challenges to both sensors and data analysis. Interesting states are not directly observable by a single sensor. In addition, sensors must be accepted by cattle and need to be robust enough to cope with a rough environment. In this article, we introduce the novel combination of heart rate and positioning sensors. Attached to neck and chest they are less interfering than accelerometers at the ankles. Exploiting the potential of such combined sensor system that records locomotion and non-spatial information from the heart rate sensor however is challenging. We introduce a novel two level method for the activity tracking focused on the duration and sequence of activity states. We combine Support Vector Machine (SVM) with Conditional Random Field (CRF) and extend Conditional Random fields by an explicit representation of duration. The SVM characterizes local activity states, whereas the CRF addresses sequences of local states to sequences incorporating spatial and non-spatial contextual knowledge. This combination provides a reliable and comprehensive identification of defined activity patterns, as well as their chronology and durations, suitable for the integration in an activity data base. This data base is used to extract physiological parameters and promises insights into internal states such as fitness, well-being and stress. Interestingly we were able to demonstrate a significant correlation between resting pulse rate and the day of pregnancy.     

Some results on disturbance attenuation for Hamiltonian systems via direct discrete‐time design

The disturbance attenuation and robust disturbance attenuation problems for Hamiltonian systems in the discrete‐time setting are considered and some new results are presented. The new results are derived utilizing the recently presented dissipativity equality obtained by adding the dissipation rate function to the classical dissipativity inequality. A selection of the dissipation rate function yields new results. These results include a condition on the dissipation structure of the system to achieve the desired disturbance attenuation level and gives direct construction of optimal control laws for any desired disturbance attenuation level. The results remove the need to solve Hamilton–Jacobi–Isaacs inequalities. Copyright © 2014 John Wiley & Sons, Ltd.     

Interaction design for supporting communication between Chinese sojourners

In our global village, distance is not a barrier anymore for traveling. People experience new cultures and face accompanying difficulties in order to live anywhere. Social support can help these sojourners to cope with difficulties, such as culture shock. In this paper, we investigate how computer-mediated communication (CMC) tools can facilitate social support when living physically separated from loved-ones in different cultures. The goal is to understand the design considerations necessary to design new CMC tools. We studied communication practices of Chinese sojourners living in the Netherlands and the use of a technology probe with a novel video communication system. These results led to recommendations which can help designers to design interactive communication tools that facilitate communication across cultures. We conclude the paper with an interactive communication device called Circadian, which was designed based on these recommendations. We experienced the design recommendations to be abstract enough to leave space for creativity while providing a set of clear requirements which we used to base design decisions upon.