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A fast and reliable diagnostic system has been developed for the detection of Mangalica meat in foods. This qualitative test is based on a recombinase polymerase amplification which can be performed on the field, in situ, where it may be necessary to determine Mangalica content in food products at once. The required equipments for the procedure are pipettes, a portable homogenizer and a portable thermostat. DNA amplification is carried out at a constant temperature, and the detection is based on antibody reaction. The detection limit is one copy of the target sequence in 1 μl reaction volume. The test can be used for uncovering falsification of local brands on the spot within a very short (25–45 min) period of time. The present approach can be adopted for the detection of other food ingredients, if the species-specific target DNA sequence is known, e.g. in case of chicken, turkey, horse, and cattle.  相似文献   
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nyn 《微型计算机》2009,(15):130-132
近年来,山寨已经成为一种“特色”,那么,什么是山寨呢?其实,品牌只有知名和不知名之分。我们平时所说的山寨品牌.就是指那些不注重产品质量,甚至靠假冒、仿冒的伪劣产品。而外置超薄DVD刻录机领域,也被山寨产品所困扰。  相似文献   
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