The trans-syn-I thymine dimer bends DNA by approximately 22 degrees and unwinds DNA by approximately 15 degrees

Chromium metabolism of lactating women was evaluated by measuring diet, breast milk, urine, and serum chromium in 17 subjects 60 d postpartum. Breast milk chromium concentration was similar for the 3 d of collection with a mean +/- SE concentration of 3.54 +/- 0.40 nmol/L (0.18 ng/mL). Dietary intake and urinary chromium values were also similar for each of the 3 collection days. Total chromium intake of lactating mothers (0.79 +/- 0.08 mumol/d) was greater than that of reference female subjects (0.48 +/- 0.02). There was a significant correlation (r = 0.84) between serum chromium and urinary chromium excretion. If a breast milk volume of 715 mL is assumed, chromium intake of exclusively breast-fed infants is < 2% of the estimated safe and adequate daily intake of 10 micrograms. In summary, breast milk chromium content is independent of dietary chromium intake and serum or urinary chromium values. Chromium intake also did not correlate with serum or urine chromium but there was a significant relationship between serum and urinary chromium concentrations.     

鄂尔多斯西缘前陆盆地巨厚黄土地区地震采集技术

鄂尔多斯西缘前陆盆地表层地质地貌极其复杂，有巨厚黄土区、黄土-沙漠区及山前砾石-黄土区，独特的黄土地貌使得地震采集面临着诸多技术难题。根据以往巨厚黄土地区地震采集经验，采用多种方法系统地开展黄土表层结构精细调查，深入研究黄土地球物理特征及激发因素与表层黄土结构之间的关系，优选激发井位和井深，确保了激发效果；采用小道距、三线小线距、高密度空间采样技术，获得了高信噪比、高分辨率地震资料。研究表明，巨厚黄土地区地震采集时应优选激发井位和岩性，合理选择激发井深；坚持多深井组合激发、单井药量不宜过大总药量要大两项基本原则；采用高密度采样技术，提高采集资料的覆盖次数和信噪比。图10表1参15     

Dual recognition of lipid A and DNA by human antibodies encoded by the VH4-21 gene: a possible link between infection and lupus

The VH4-21 (V4-34) gene segment, a member of the VH4 family, is expressed early in B-cell maturation and is utilized by approximately 6% of normal adult B lymphocytes. This prevalence indicates an importance of VH4-21 in the B-cell repertoire. The gene also encodes certain autoantibodies being mandatory for pathological IgM anti-red cell antibodies directed against the I/i antigen, and also capable of encoding anti-DNA antibodies. Recognition of I/i antigen or DNA appears to be via two distinct sites on VH, with I/i binding mediated by sequences in the framework region, and DNA binding correlating with the presence of positively charged amino acids in complementarity-determining region 3. However, these positively charged residues appear to suppress the ability of the framework region to interact with I/i, rendering a single sequence monospecific for I/i or DNA. The IgM anti-DNA antibodies also recognize bacterial lipid A, whereas the anti-I/i antibodies do not, indicating that CDR3 may be involved in binding the negatively charged lipid A. Structural similarities between the DNA backbone and lipid A provide a possible explanation for this cross-reactivity. This dual recognition of bacterial antigen and autoantigen provides a potential link between infection and autoimmunity.     

Interleukin-12 induces relapse in experimental allergic encephalomyelitis in the Lewis rat

Acute, monophasic experimental allergic encephalomyelitis (EAE) in the Lewis rat shows pathological similarities to the human disease multiple sclerosis (MS). Rats that recover from EAE are essentially resistant to disease reinduction, unlike MS in which relapses are frequently associated with common bacterial and viral infections. As macrophage-derived interleukin (IL)-12 is a critical component of innate resistance to bacterial infection and appears to directly activate encephalitogenic T cells in vivo, the ability of this cytokine to reinduce paralysis in EAE was examined. Paralytic disease was exacerbated by intraperitoneal IL-12 administration and could be reinduced up to 1 week after recovery from the primary clinical episode. Concomitant with worsening of initial clinical signs and relapse was an increase in the ratio of macrophages to T cells in brain stem perivascular cuffs and the expression of inducible nitric oxide synthase in cells with both macrophage and microglial morphology. These findings suggest that IL-12 may contribute to macrophage-mediated disease exacerbation and relapse in patients with MS.     

Behavioral factors affecting exposure potential for household cleaning products

Variation in repeated nucleotide sequences (RNSs) at the level of entire families assayed by Southern blot hybridization is remarkably low within species and is a powerful tool for scrutinizing the origin of allopolyploid taxa. Thirty-one clones from RNSs isolated from different Triticeae genera were used to investigate the genome constitution of South American Elymus. One of these clones, pHch2, preferentially hybridized with the diploid H genome Hordeum species. Hybridization of this clone with a worldwide collection of Elymus species with known genome formulas showed that pHch2 clearly discriminates Elymus species with the H genome (StH, StHH, StStH, and StHY) from those with other genome combinations (StY, StStY, StPY, and StP). Hybridization with pHch2 indicates the presence of the H genome in all South American Elymus species except Elymus erianthus and Elymus mendocinus. Hybridization with additional clones that revealed differential restriction fragments (marker bands) for the H genome confirmed the absence of the H genome in these species. Differential restriction fragments for the Ns genome of Psathyrostachys were detected in E. erianthus and E. mendocinus and three species of Leymus. Based on genome constitution, morphology, and habitat, E. erianthus and E. mendocinus were transferred to the genus Leymus.     

新型清洁压裂液的实验室合成

研制出一种新型的双子季铵盐表面活性剂———N,N-双十八烷基-N,N,N,N-四醇乙基-二溴乙二铵(Gemini-OHAB),对用Gemini-OHAB及其它辅剂配制的新型压裂液体系性能进行了研究。结果表明:该体系具有良好的粘弹性、悬砂性能、低温溶解性以及抗温稳定性能;而且由于双子季铵盐表面活性剂中引入了醇乙基,该新型压裂液体系解决了传统季铵盐类清洁压裂液体系的低温不溶性及高生物毒性,也克服了甜菜碱类清洁压裂液体系抗温性能低的缺点,具有良好的市场应用前景。     

《航空油料及其添加剂实用红外光谱图集》的研究

1　前言红外光谱分析技术是化学、医药、生化、石油化工等研究工作的重要分析手段。红外光谱在航空油料及其添加剂的研制、生产、分析鉴定等方面有着广泛的应用。利用红外光谱技术通过检索红外谱图集 ,可以快速、准确地判定化合物的官能团 ,从而鉴别化合物的结构。国外已有的Ｓａｄｔｌｅｒ红外光谱图集无论是光盘还是图书的价格都极为昂贵 ,引进困难。而且Ｓａｄｔｌｅｒ红外光谱图集内容庞杂 ,对于没有计算机检索工具的航空油料领域的研究者来说 ,在成千上万张红外谱图中 ,查对寻找一张需要的谱图 ,实在是一件繁琐的工作。而国内只有一些…