Early traces of usability as a science and as a profession

Shackel’s paper [Shackel, B., 1991. Usability – context, framework, definition, design and evaluation. In: Shcakel, B., Richardson, S. (Eds.), Human Factors for Informatics Usability. Cambridge University Press, Cambridge, UK] is reviewed in an attempt to assess his contribution to the development of usability as a science and as a profession. Usability related research is first situated in the period around 1990. The contributions to usability as a science then are addressed via Professor Shackel’s definition and evaluation of usability. Finally, his contribution toward usability as a profession is acknowledged via his view of usability in the light of wider business goals.     

Attention web designers: You have 50 milliseconds to make a good first impression!

Three studies were conducted to ascertain how quickly people form an opinion about web page visual appeal. In the first study, participants twice rated the visual appeal of web homepages presented for 500 ms each. The second study replicated the first, but participants also rated each web page on seven specific design dimensions. Visual appeal was found to be closely related to most of these. Study 3 again replicated the 500 ms condition as well as adding a 50 ms condition using the same stimuli to determine whether the first impression may be interpreted as a 'mere exposure effect' (Zajonc 1980). Throughout, visual appeal ratings were highly correlated from one phase to the next as were the correlations between the 50 ms and 500 ms conditions. Thus, visual appeal can be assessed within 50 ms, suggesting that web designers have about 50 ms to make a good first impression.     

Brain levels of <Emphasis Type="Italic">N</Emphasis>-acylethanolamine phospholipids in mice during pentylenetetrazol-induced seizure

The N-acylethanolamine phospholipids (NAPE) are precursors for N-acylethanolamines (NAE), including anandamide (20∶4-NAE), which is a ligand for the cannabinoid receptors. Previously, NAPE were believed to be found only in injured tissue, e.g., after neurodegenerative insults. Neuronal injury may occur in response to seizure activity. Therefore, we investigated the effect of pentylenetetrazol (PTZ)-induced seizures in PTZ-kindled mice on the level of NAPE in the brain. Male NMRI mice were kindled with PTZ injections 3 times/wk, thereby developing clonic seizures in response to PTZ. Mice were killed within 30 min after the clonic seizure on the test day (12th injection) and the brains were collected. Eight species of NAPE were analyzed as the glycerophospho-N-acylethanolamines by high-performance liquid chromatography-coupled electrospray ionization mass spectrometry. No effect of the PTZ kindling on the NAPE levels in murine brains was observed. Total NAPE in control mice cortex (n=4) was 16.4±3.0 μmol/g wet weight of which 20∶4-NAPE accounted for 3.6 mol%, and the major species was 16∶0-NAPE, accounting for 52.1 mol%. Determination of the activity of NAPE-hydrolyzing phospholipase D and of N-acyltransferase in brain membrane preparations from adult and 3-d-old mice revealed an enzyme pattern in the adult mice that was favorable for NAE accumulation as opposed to NAPE accumulation. Thus, there was no difference in NAPE levels; at present, however, this does not exclude that NAE may accumulate during seizure.     

Design of an individually controlled system for an optimal thermal microenvironment

Individually controlled microenvironment has potential to satisfy more occupants in a space compared to a total volume uniform environment typically used at present. The performance of an individually controlled system comprising a convection-heated chair, an under-desk radiant heating panel, a floor radiant heating panel, an under-desk air terminal device supplying cool air, and a desk-mounted personalized ventilation as used and identified by 48 human subjects was studied using a thermal manikin at room temperatures of 20 °C, 22 °C and 26 °C. At a room air temperature of 20 °C, the maximum whole-body heating effect of the heating chair, the under-desk heating panel, and the floor heating panel corresponded to the effect of a room temperature increase of 5.2 °C, 2.8 °C, and 2.1 °C, respectively. The effect was 5.9 °C for the combination of the three heating options. The higher the room air temperature, the lower the heating effect of each heating option or heating combination. The maximum whole-body cooling effect of the tested system was only −0.8 °C at a room air temperature of 26 °C. The heating and cooling capacity of the individually controlled system were identified. These results, analyzed together with results obtained from human subject experiments, reveal that both the heating and the cooling capacity of the individually controlled system need to be increased in order to satisfy most occupants in practice.     

Synthesis of α‐Carbolines Starting from 2,3‐Dichloropyridines and Substituted Anilines

9H‐α‐Carbolines have been prepared via consecutive intermolecular Buchwald–Hartwig reaction and Pd‐catalyzed intramolecular direct arylation from commercially available 2,3‐dichloropyridines and substituted anilines. The combination of a high reaction temperature (180 °C) and the use of DBU were found to be crucial for the intramolecular direct arylation reactions of the 3‐chloro‐N‐phenylpyridin‐2‐amines as no reaction was observed at 120 °C and 180 °C using different inorganic and other organic bases. On the other hand, nitrogen‐methylated pyridine analogues of these substrates {N‐[3‐chloro‐1‐methylpyridin‐2(1H)‐ylidene]anilines} do undergo ring closure at 120 °C, with K₃PO₄ as base, affording the respective 1‐methyl‐1H‐α‐carbolines in good yields.     

The 21st-Century Welfare City: Aalborg + Copenhagen,Denmark

Under the scientific eye of satellite surveillance, the world's urban conglomerations often appear heterogeneous. On the ground, differences and special features of a city remain of utmost importance to the quality of life of its citizens. Hans Kiib and Gitte Marling assess how the welfare system in Denmark, which is so dear to many Danes, is holding up. If the physical, Modernist urban model of the welfare city is to endure, can it withstand the irresistible, less tangible pull of consumption and aspirational individualised lifestyles that are penetrating the globe worldwide? Copyright © 2005 John Wiley & Sons, Ltd.     

The acylation and phosphorylation pattern of lipid A from Xanthomonas campestris strongly influence its ability to trigger the innate immune response in Arabidopsis

Lipopolysaccharides (LPSs) are major components of the cell surface of Gram-negative bacteria. LPSs comprise a hydrophilic heteropolysaccharide (formed by the core oligosaccharide and the O-specific polysaccharide) that is covalently linked to the glycolipid moiety lipid A, which anchors these macromolecules to the external membrane. LPSs are one of a group of molecules called pathogen-associated molecular patterns (PAMPs) that are indispensable for bacterial growth and viability, and act to trigger innate defense responses in eukaryotes. We have previously shown that LPS from the plant pathogen Xanthomonas campestris pv. campestris (Xcc) can elicit defense responses in the model plant Arabidopsis thaliana. Here we have extended these studies by analysis of the structure and biological activity of LPS from a nonpathogenic Xcc mutant, strain 8530. We show that this Xcc strain is defective in core completion and introduces significant modification in the lipid A region, which involves the degree of acylation and nonstoichiometric substitution of the phosphate groups with phosphoethanolamine. Lipid A that was isolated from Xcc strain 8530 did not have the ability to induce the defense-related gene PR1 in Arabidopsis, or to prevent the hypersensitive response (HR) that is caused by avirulent bacteria as the lipid A from the wild-type could. This suggests that Xcc has the capacity to modify the structure of the lipid A to reduce its activity as a PAMP. We speculate that such effects might occur in wild-type bacteria that are exposed to stresses such as those that might be encountered during plant colonization and disease.     

Bioactivities of açaí (Euterpe precatoria Mart.) fruit pulp,superior antioxidant and anti-inflammatory properties to Euterpe oleracea Mart.

There are two predominant palm tree species producing edible fruit known as “açaí” found widely dispersed through the Amazon: Euterpe oleracea Mart. and Euterpe precatoria Mart. They differ from each other in terms of how the plants grow and their phytochemical composition. E. oleracea (EO) has received considerable attention as a “super fruit” because of its high antioxidant capacity, while studies on E. precatoria (EP) remain rare. In this study, the antioxidant and anti-inflammatory activities of EP fruit pulps were evaluated by different assays including a series of oxygen radical absorbance capacity (ORAC) based assays, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the cell-based antioxidant protection in erythrocyte (CAP-e) assay, as well as the nuclear factor-kappa B (NF-κB) secreted embryonic alkaline phosphatase (SEAP) assay. Total phenolics were also measured as an indication of the total phenol content. For comparative purposes, the EO fruit pulp was included. The antioxidant capacity of the EP fruit pulp was determined to be superior to the EO fruit pulp in every chemical based assay. In the cell-based CAP-e assay, the EP fruit pulp showed a dose-dependent inhibition against oxidative damage with an IC₅₀ of 0.167 g/l. In the SEAP reporter assay, the EP fruit pulp polyphenol-rich extracts inhibited lipopolysaccharide (LPS)-induced NF-κB activation by 23% (p < 0.05) at 20 μg/ml, whereas the extract of the EO fruit pulp did not show a significant inhibitory effect at comparable doses. In addition, carotenoids were quantified for the first time in EP, since EP has high scavenging capacity against singlet oxygen.     

A critical examination of some experimental variables in the 2-thiobarbituric acid (TBA) test for lipid oxidation in meat products

By comparison of various procedures for the determination of TBARS (2-thiobarbituric-acid-reactive substances) in non-cured meat samples, it was found that a direct extraction procedure with trichloroacetic acid gave significantly higher reaction yields than a rapid Kjeltec steam distillation procedure (50 ml distillate in 2.5 min). When optimizing the distillation procedure with regard to distillation principle (heating by thermal conductivity or steam injection) and rate, the reaction yield was more affected by changes in distillation principle than by changes in distillation rate. Simple distillation (conduction heating) yielded significantly higher TBA values than steam distillation at the same distillation rate. The reproducibility of the distillation procedure was found to be about 5–10 times poorer than of the extraction procedure and, because of the high reaction yields and robustness of the extraction procedure, the use of the extraction procedure is recommended with food matrices such as non-cured meat samples with less than 14–18% fat and no artificial colorants.