Variability in the proliferative responsiveness of cultured human vascular smooth muscle cells to alpha-thrombin

alpha-Thrombin, a key enzyme of the coagulation cascade, is also a potent mitogen for many cell types. In the present study, the responsiveness to alpha-thrombin of cultured human vascular smooth muscle cells (HVSMC) derived from either vein or normal and atherosclerotic arteries was investigated. All HVSMC populations examined responded mitogenically to alpha-thrombin. However, the extent of this response varied between different cell populations. No significant differences were observed between HVSMC derived from vein versus artery or atherosclerotic versus normal tissues. The responsiveness of a specific HVSMC culture to alpha-thrombin was not affected by cell density and remained constant over several passages. Unlike platelet-derived growth factor BB (PDGF-BB), alpha-thrombin did not exhibit any significant chemotactic effects on HVSMC or induce their anchorage independent growth in semi-solid medium. The hypothesis that the observed variability in HVSMC responsiveness to alpha-thrombin is due to the heterogeneity of cultured HVSMC is raised and discussed.     

Tissue factor pathway inhibitor in endothelial cells colocalizes with glycolipid microdomains/caveolae. Regulatory mechanism(s) of the anticoagulant properties of the endothelium

Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor.factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC with caveolin, urokinase-type plasminogen activator receptor, and glycosphingolipids. The localization of TFPI in caveolae in resting endothelium is proved by double immunogold electron microscopy for TFPI and caveolin. After ultracentrifugation of rat lung or EC homogenates through density gradients of Nycodenz, TFPI was highly enriched at densities of 1.05 to 1.08 g/mL, together with caveolin and alkaline phosphatase. By ELISA, more than half of the cellular TFPI was detected in Triton X-100-insoluble extracts of EC. TFPI incorporates [1-3H]ethanolamine and is cleaved from the cell surface by phosphatidylinositol-phospholipase C, indicating a specific glycosylphosphatidylinositol-anchorage mechanism for TFPI in the plasma membrane. Clustering of TFPI and its localization in caveolae are dependent on the presence of cholesterol in the membrane. Agonist-induced stimulation of EC caused marked changes of distribution for both TFPI and caveolin at subcellular level, with subsequent increase of the cell surface-associated inhibitory activity toward tissue factor.factor VIIa. Our findings suggest that, beside their function in transcytosis, potocytosis, cell surface proteolysis, and regulation of signal transduction, caveolae also play a direct role in the regulation of EC anticoagulant properties.     

Membrane degumming of crude rice bran oil: Pilot plant study

The procedure for the classical chemical refining of vegetable oils consists of degumming, alkali neutralization, bleaching, and deodorization. Conventional refining of rice bran oil using alkali gives oil of acceptable quality, but the refining losses are very high. A critical work has been carried out to study the application of membrane technology in the pretreatment of crude rice bran oil. Oils intended for physical refining should have a low phosphorus content, and this is not readily achievable by the conventional acid/water degumming process. The application of membrane technology for the pretreatment of rice bran oil has been investigated. The process has already been successfully applied to other vegetable oils. Ceramic membranes, which are important from the commercial point of view, were examined for this purpose. The results showed that the membrane‐filtered oils met the requirements of physical refining, with a substantial reduction in color. It was observed that most of the waxy material was also rejected. Experiments were carried out to establish the relationship between permeate flux and rejection with membrane pore size, trans‐membrane pressure and micellar solute concentration.     

CRT and LCD monitor and process materials evaluated for environmental improvement

Decision rules have been developed and applied to the bills of materials of a color cathode‐ray tube (CRT) and a liquid‐crystal‐display (LCD) desktop monitor to determine which product and process materials will be evaluated in an environmental life‐cycle assessment. Materials of significant mass, of technological importance, and of potential environmental impact are targeted. The list of materials identified are those for which life‐cycle inventory data will be obtained for the materials extraction and materials processing life‐cycle stages of a CRT and an LCD. Additionally, materials identified will also be used to represent life‐cycle impact in terms of resource consumption, as well as surrogates for occupational health impacts.     

Analysis of Elevated Temperature Flow Behavior of 316LN Stainless Steel Under Compressive Loading

To understand the flow behavior of a newly developed austenitic stainless steel 316LN with 0.14 wt% nitrogen, isothermal compression tests have been carried out in the hot working domain. From the analysis of flow behavior, it is observed that the nitrogen enhanced steel, in its hot working domain exhibits strain and strain rate hardening with thermal softening. The flow behavior analysis also demonstrates the coupled effect of strain–temperature and strain rate temperature on the flow stress. To depict the flow behavior of the material, strain compensated Arrhenius (SCA) equation and Model D8A have been used. The SCA predicts the flow curves with an average absolute error of 9.27% and a correlation coefficient of 0.977, whereas the prediction by Model D8A gives the average absolute relative error as 10.86% with a correlation coefficient of 0.966. For high temperature and intermediate strain rate, both Model D8A and SCA equation depict the flow behavior of 316L (0.14)N SS with good correlation and generalization. However, at low temperature and high strain rate domain, both the models are unable to depict the behavior; this is attributed to the fact that the material shows two slope behaviors where the constants have been calculated assuming a linear relationship between stress and strain rate.     

Microscale analysis of patterning reactions via FTIR imaging: Application to intelligent hydrogel systems

A novel application of FTIR imaging for real-time characterization of patterning polymerization processes with microscale spatial resolution is presented. These methods will enable the microscale analysis of the reactions of polymeric systems with various substrates and devices. Specifically, intelligent hydrogels containing ionic groups (pH responsive) and poly(ethylene glycol) have been micropatterned onto gold surfaces, and the free-radical polymerization reaction has been characterized. It was demonstrated that differences in the reaction rates across a patterned region could be successfully resolved and characterized. This novel characterization method based on FTIR imaging will facilitate the optimization of integration processes of patterned polymeric films leading to enhanced (and reproducible) application of these materials as functional components in a variety of microdevices.     

A single-step polymerization method for poly(β-amino ester) biodegradable hydrogels

A novel one-step method for the preparation of poly(β-amino ester) hydrogels has been developed. The one-step method utilizes a Michael type addition reaction between a difunctional diacrylate and a tetrafunctional primary diamine to create a crosslinked biodegradable hydrogel. It was shown that the molar ratio of the reactive acrylate to amine controlled the extent of polymerization and crosslinking density of the hydrogel system, which enabled for the end properties of the network (e.g., swelling, degradation, and mechanical) to be tuned.