Role of p53 gene in colorectal neoplasms

A prospective study on the role of the p53 gene in sporadic colorectal neoplasms is presented and the level of mutant p53 protein was measured in the tissue removed during colonoscopy from: patients previously operated for colorectal malignant neoplasms, patients with active neoplasms, first degree relatives and during regular checks. 72% of patients with an active tumour showed a positive p53 and 38% in follow-up checks. Longer follow-up periods and a major number of patients are necessary to assess the prognostic importance of the p53 protein.     

Evaluating the impact of smart technologies on harbor’s logistics via BPMN modeling and simulation

A smart Information and Communication Technology (ICT) enables a synchronized interplay of different key factors, aligning infrastructures, consumers, and governmental policy-making needs. In the harbor’s logistics context, smart ICT has been driving a multi-year wave of growth. Although there is a standalone value in the technological innovation of a task, the impact of a new smart technology is unknown without quantitative analysis methods on the end-to-end process. In this paper, we first present a review of the smart ICT for marine container terminals, and then we propose to evaluate the impact of such smart ICT via business process model and notation (BPMN) modeling and simulation. The proposed approach is discussed in a real-world modeling and simulation analysis, made on a pilot terminal of the Port of Leghorn (Italy).     

Distribution of nitric oxide synthase immunoreactivity in the mouse brain

Nitric oxide (NO) is a gaseous intercellular messenger with a wide range of neural functions. NO is synthesized by activation of different isoforms of nitric oxide synthases (NOS). At present NOS immunoreactivity has been described in mouse brain in restricted and definite areas and no detailed mapping studies have yet been reported for NOS immunoreactivity. We have studied the distribution of neuronal NOS-containing neurons in the brain of three months male mice, using a specific commercial polyclonal antibody against the neuronal isoform of nitric oxide synthase (nNOS). Neuronal cell bodies exhibiting nNOS immunoreactivity were found in several distinct nuclei throughout the brain. The neurons that were positively stained exhibited different intensities of reaction. In some brain areas (i.e., cortex, striatum, tegmental nuclei) neurons were intensely stained in a Golgi-like fashion. In other regions, immunoreactive cells are moderately stained (i.e., magnocellular nucleus of the posterior commissure, amygdaloid nucleus, interpeduncular nucleus, lateral periaqueductal gray) or weakly stained (i.e., vascular organ of the lamina terminalis, hippocampus, inferior colliculus, reticular nucleus). In the mouse, the NO-producing system appears well developed and widely diffused. In particular, nNOS immunoreactive neurons seem chiefly present in several sensory pathways like all the nuclei of the olfactory system, as well as in many regions of the lymbic system. These data suggest a widespread role for the NO system in the mouse nervous system.     

Abstract reduction in directed model checking CCS processes

Model checking tools face a combinatorial blow up of the state-space (commonly known as the state explosion problem) that must be addressed to formally verify concurrent systems. We propose an approach combining abstraction techniques and heuristic search to overcome the problem above. In particular, heuristic search can avoid the bottleneck of the exhaustive exploration of the global state graph of a system, while retaining the advantages of abstraction techniques.     

The connection between Bell's inequalities based on probabilities and those based on correlations

Abstract

Violation of Bell inequalities is widely regarded as a definitive test for non-locality. However, Bell correlational inequalities must always be satisfied by all jointly present, cross-correlated data. The correlations of variable pairs obtained in repeated runs are not cross-correlated in this way and are not required to satisfy the Bell inequality. In addition, by using information regarded as non-local, proper joint correlations may be computed among counterfactual and measured variables. These correlations satisfy the Bell inequality, but are spatially non-stationary in angle. By using a simple symmetry condition, such considerations may be extended to inequalities in probabilities. The latter may be derived directly from correlational inequalities developed by Clauser, Horne, Shimony and Holt (CHSH). Violation of either correlational or probabilistic Bell inequalities then implies that the Bell correlation cannot be accounted for by a stochastic process that is spatially stationary in angle coordinates. However, other processes may still be allowed.     

Conjugation of amino-bioactive glasses with 5-aminofluorescein as probe molecule for the development of pH sensitive stimuli-responsive biomaterials

Bioceramics, such as silica-based glasses, are widely used in bone and teeth restoration. Nowadays, the association between nanotechnology and pharmacology is one of the most promising research fields in cancer therapy. The advanced processing methods and new chemical strategies allow the incorporation of drugs within them or on their functionalized surfaces. Bioceramics can act as local drug delivery systems to treat bone and teeth diseases. The present paper reports data related to the development of a pH-stimuli responsive bioactive glass. The glass conjugation with 5-aminofluorescein (5-AF), through a pH-sensitive organic spacer, allows to produce a pH-responsive bioactive biomaterial: when it is exposed to specific pH changes, it can favour the release of 5-AF directly at the target site. 5-AF has been chosen as a simple, low cost, non toxic model to simulate doxorubicin, an anticancer drug. As doxorubicin, 5-AF contains an amino group in its structure in order to form an amide bond with the carboxylic functionalities of the glass. Raman spectroscopy and thermal analysis confirm the glass conjugation of 5-AF by means of an amide bond; the amount of 5-AF loaded was very high (≈65 and 44 wt%). The release tests at two different pH (4.2 and 7.4) show that the amount of released 5-AF is higher at acid pH with respect to physiological one. This preliminary datum evidenced that a pH-sensitive drug delivery system has been developed. The low amount of 5-AF released (<1 wt% of the total 5-AF) is due to the very low solubility of 5-AF in aqueous medium. This disadvantage, may be overcome in a dynamic environment (physiological conditions), where it is possible to obtain a drug release system ensuring an effective therapeutic dose for long times and, at the same time, avoiding the drug toxicity.     

Lidar measurements taken with a large-aperture liquid mirror. 2. Sodium resonance-fluorescence system

Sodium resonance-fluorescence lidar is an established technique for measuring atmospheric composition and dynamics in the mesopause region. A large-power-aperture product (6.6-W m(2)) sodium resonance-fluorescence lidar has been built as a part of the Purple Crow Lidar (PCL) at The University of Western Ontario. This sodium resonance-fluorescence lidar measures, with high optical efficiency, both sodium density and temperature profiles in the 83-100-km region. The sodium lidar operates simultaneously with a powerful Rayleigh- and Raman-scatter lidar (66 W m(2)). The PCL is thus capable of simultaneous measurement of temperature from the tropopause to the lower thermosphere. The sodium resonance-fluorescence lidar is shown to be able to measure temperature to an absolute precision of 1.5 K and a statistical accuracy of 1 K with a spatial-temporal resolution of 72 (km s) at an altitude of 92 km. We present results from three nights of measurements taken with the sodium lidar and compare these with coincident Rayleigh-scatter lidar measurements. These measurements show significant differences between the temperature profiles derived by the two techniques, which we attribute to variations in the ratio of molecular nitrogen to molecular oxygen that are not accounted for in the standard Rayleigh-scatter temperature analysis.     

Simple high-performance liquid chromatographic method for determination of losartan and E-3174 metabolite in human plasma, urine and dialysate

A simple high-performance liquid chromatographic (HPLC) method was developed for the determination of losartan and its E-3174 metabolite in human plasma, urine and dialysate. For plasma, a gradient mobile phase consisting of 25 mM potassium phosphate and acetonitrile pH 2.2 was used with a phenyl analytical column and fluorescence detection. For urine and dialysate, an isocratic mobile phase consisting of 25 mM potassium phosphate and acetonitrile (60:40, v/v) pH 2.2 was used. The method demonstrated linearity from 10 to 1000 ng/ml with a detection limit of 1 ng/ml for losartan and E-3174 using 10 microl of prepared plasma, urine or dialysate. The method was utilized in a study evaluating the pharmacokinetic and pharmacodynamic effects of losartan in patients with kidney failure undergoing continuous ambulatory peritoneal dialysis (CAPD).