Investigation of antimicrobial properties and in-vitro bioactivity of Ce3+-Sr2+dual-substituted nano hydroxyapatites

Dual doped calcium apatite has been widely focused as it enhances the osteoconductive property for the possible applications in orthopedic and dental implants. In this work, we investigate the antimicrobial and bioactive properties of cerium/strontium (Ce³⁺/Sr²⁺) co-substituted hydroxyapatite (HA) nanoparticles synthesized by sol-gel assisted precipitation method. The structure, morphology, functional groups, photoluminescence, and thermal stability of the developed systems are examined. The comparative studies performed among the pure HA, Sr²⁺, and Ce³⁺-substituted HA nanoparticles illustrate higher antibacterial activity with lowered apatite-forming ability and biocompatibility for the Ce³⁺-substituted HA. However, the Ce³⁺/Sr²⁺co-substituted HA exhibits better biocompatibility, apatite-forming ability, and good antimicrobial properties. Sr²⁺ ion inclusion leads to better biological properties and compromise the cytotoxic nature of the Ce³⁺-HA. In addition, the Ce³⁺/Sr²⁺-HA nanoparticles prevent thermal decomposition up to 700°C, pointing also toward the possibility of this co-substituted HA in bone implant applications.