A Possible Industrial Solution to Ferment Lignocellulosic Hydrolyzate to Ethanol: Continuous Cultivation with Flocculating Yeast

The cultivation of toxic lignocellulosic hydrolyzates has become a challenging research topic in recent decades. Although several cultivation methods have been proposed, numerous questions have arisen regarding their industrial applications. The current work deals with a solution to this problem which has a good potential application on an industrial scale. A toxic dilute-acid hydrolyzate was continuously cultivated using a high-cell-density flocculating yeast in a single and serial bioreactor which was equipped with a settler to recycle the cells back to the bioreactors. No prior detoxification was necessary to cultivate the hydrolyzates, as the flocks were able to detoxify it in situ. The experiments were successfully carried out at dilution rates up to 0.52 h⁻¹. The cell concentration inside the bioreactors was between 23 and 35 g-DW/L, while the concentration in the effluent of the settlers was 0.32 ± 0.05 g-DW/L. An ethanol yield of 0.42–0.46 g/g-consumed sugar was achieved, and the residual sugar concentration was less than 6% of the initial fermentable sugar (glucose, galactose and mannose) of 35.2 g/L.     

Modulation of silica layer properties by varying the granulometric state of tetraethyl orthosilicate precursor aerosols during combustion chemical vapor deposition (CCVD)

Abstract

For the purpose of silica surface layer modulation, a pneumatic-controlled two-substance atomizer with inertia-based coarse droplet separation was operated at different system pressures for tetraethyl orthosilicate precursor aerosol supply during combustion chemical vapor deposition. A comprehensive testing study was performed to characterize the atomizer’s performance characteristics, initial precursor aerosols at the atomizer’s outlet, transformed aerosols before combustion, combustion aerosols and formed layers. Laser diffraction spectrometry, differential electrical mobility analyses and condensation particle counting were used for aerosol characterization with regard to particle size and particle production quantities. Layers were characterized by scanning electron microscopy, atomic force microscopy, spectral ellipsometry, water contact angle measurements and light transmission concerning geometric properties (thickness, surface structure and roughness) and physical behaviors (i.e., optical behaviors, hydrophobicity). Results show a quasi-linear relationship of the ejection mass flow of the pneumatic-controlled atomizer and geometric layer properties which again show a direct relationship to the physical properties. No correlation was found between the aerosols before combustion and the combustion aerosols since the majority of combustion aerosol particles are synthesized solely from the gas phase based on evaporated precursor material.

Copyright © 2020 American Association for Aerosol Research     

Bribery in voting with CP-nets

We investigate the computational complexity of finding optimal bribery schemes in voting domains where the candidate set is the Cartesian product of a set of variables and voters use CP-nets, an expressive and compact way to represent preferences. To do this, we generalize the traditional bribery problem to take into account several issues over which agents vote, and their inter-dependencies. We consider five voting rules, three kinds of bribery actions, and five cost schemes. For most of the combinations of these parameters, we find that bribery in this setting is computationally easy.     

Active learning paradigms for CBIR systems based on optimum-path forest classification

This paper discusses methods for content-based image retrieval (CBIR) systems based on relevance feedback according to two active learning paradigms, named greedy and planned. In greedy methods, the system aims to return the most relevant images for a query at each iteration. In planned methods, the most informative images are returned during a few iterations and the most relevant ones are only presented afterward. In the past, we proposed a greedy approach based on optimum-path forest classification (OPF) and demonstrated its gain in effectiveness with respect to a planned method based on support-vector machines and another greedy approach based on multi-point query. In this work, we introduce a planned approach based on the OPF classifier and demonstrate its gain in effectiveness over all methods above using more image databases. In our tests, the most informative images are better obtained from images that are classified as relevant, which differs from the original definition. The results also indicate that both OPF-based methods require less user involvement (efficiency) to satisfy the user's expectation (effectiveness), and provide interactive response times.     

Long‐Range Domain Structure and Symmetry Engineering by Interfacial Oxygen Octahedral Coupling at Heterostructure Interface

In epitaxial thin film systems, the crystal structure and its symmetry deviate from the bulk counterpart due to various mechanisms such as epitaxial strain and interfacial structural coupling, which is accompanyed by a change in their properties. In perovskite materials, the crystal symmetry can be described by rotations of sixfold coordinated transition metal oxygen octahedra, which are found to be altered at interfaces. Here, it is unraveled how the local oxygen octahedral coupling at perovskite heterostructural interfaces strongly influences the domain structure and symmetry of the epitaxial films resulting in design rules to induce various structures in thin films using carefully selected combinations of substrate/buffer/film. Very interestingly it is discovered that these combinations lead to structure changes throughout the full thickness of the film. The results provide a deep insight into understanding the origin of induced structures in a perovskite heterostructure and an intelligent route to achieve unique functional properties.     

Dehydrogenase Enzymes Associated to Glycolysis in Beef Carcasses Stored at 0 °C

After the death of an animal, cell metabolism is controlled locally. The post-mortem oxygen depletion increases the glycolytic activity and lactate production. However, many mechanisms of post-mortem metabolic regulation have not been fully investigated in beef carcasses. In this work, we studied the post-mortem glycolytic behavior (including lactate dehydrogenase) and three dehydrogenase associated to glycolysis (glycerophosphate dehydrogenase, glucose 6-phosphate dehydrogenase, and glycerol dehydrogenase) by using cytochemistry techniques in three fast-twitch muscles (M. longissimus dorsi, M. semimembranosus, and M. cutaneus trunci) of carcasses stored at 0 °C. Our results indicate that glycolysis depends on the type of muscle. The post-mortem glycolytic flux and lactate dehydrogenase activity of M. cutaneus trunci was the lowest of the three muscles studied. Of the other dehydrogenases analyzed, only glycerophosphate and glycerol dehydrogenase showed clear cytochemical reaction. Glucose 6-phosphate dehydrogenase was not used by muscles very much. The glycerophosphate dehydrogenase was the strongest enzymatic activity correlated to the post-mortem glycolytic flux. In addition, a relationship between glycerophosphate dehydrogenase and glycerol dehydrogenase was detected by using a multiple regression model. This phenomenon was studied by using bioinformatics tools, suggesting that glycerophosphate dehydrogenase could oxidize the glycerol in bovine fast-twitch muscles.     

Comparative assessment of reproductive impairment in the gastropod mollusc Littorina littorea along the Belgian North Sea coast

In this study we present the results of an intersex survey of Littorina littorea along the Belgian coast. Levels of female intersex and sterility were determined to assess TBT related adverse effects. In addition, we determined the levels of male penis shedding and trematode infestation and investigated the morphology of the shell. Significant differences were found for all these variables which clearly differentiated periwinkles from Zeebrugge (B2) from those at other locations. Intersex index (ISI) values were relatively low (i.e. 0.00-0.39), except at B2 where they ranged up to 3.52, the highest value ever reported in literature. Consequently, female reproductive impairment at B2 was severe. Indeed, up to 95% of female periwinkles were sterile at B2. In addition, 61% of the male periwinkles had shed their penis. Furthermore, no trematode infestation could be detected at B2 and specimens from this location had the largest and heaviest shells, which may be related to population demography and/or a different use of energy budgets.     

Synthesis, Molecular Characterization and Preliminary Antioxidant Activity Evaluation of Quercetin Fatty Esters

Quercetin shows interesting pharmacological effects, but its use in topical applications is limited by its low skin permeability and solubility. In this work, the synthesis of highly lipophilic quercetin esters with oleic, linoleic and linolenic acid useful as topical quercetin prodrugs is reported. Partial OH esterification is advisable to maintain the antioxidant activity of these compounds; tetraesters and triesters can be achieved by modulating the reaction conditions utilized for the total esterification of quercetin. The chemical structures of the esters were proven by spectroscopic techniques; quantum chemical NMR calculation were mandatory to unequivocally assign the free position in triesters. Finally, the antioxidant activity of all the synthesized compounds was determined by the 2,2-diphenyl-1-picryl-hydrazyl method and by 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) assay.