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排序方式: 共有142条查询结果,搜索用时 15 毫秒
1.
Abidur Rahman Makoto Kobayashi Kotaro Sugimoto Yuta Endo Manabu Kojima Shigenori Furukawa Takafumi Watanabe Shu Soeda Yuko Hashimoto Keiya Fujimori Hideki Chiba 《International journal of molecular sciences》2021,22(7)
Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases. 相似文献
2.
The absorption spectrum of poly(3-alkylthiophene) gel changes drastically in association with a volume instability upon changing the solvent composition and also the temperature. In the expanded state in chloroform and at high temperature, the photoluminescence is much enhanced compared with the shrunken state in ethanol and at low temperature. This gel chromism and anomalous luminescence are discussed in terms of the change of effective conjugation length induced by the steric hindrance and the dynamics of the photoexcited species. 相似文献
3.
We observed change in distance between two droplets in each step after application of large multi-step shear strains. Experiments were performed using a sliding plate apparatus. Large step shear strains were applied to two polyisobutylene droplets in poly(dimethyl siloxane) matrix in the same plane between the plates. The distance between the two droplets decreases with increasing the total shear strain, which is given by the product of the step strain magnitude and the number of application of the step strains. The two droplets coalesce when the distance becomes less than the diameter of the droplets. The slope for plots of the distance versus the total strain is independent of the step strain magnitude. This indicates that the effect per unit strain on the distance is the same, irrespective of the strain magnitude. It is suggested that a stronger hydrodynamic interaction between the droplets is the main cause for the droplet approach. 相似文献
4.
Shigenori Egusa 《Journal of Materials Science》1988,23(8):2753-2760
Four kinds of polymer matrix composites (filler, E-glass or carbon fibre cloth; matrix, epoxy or polyimide resin) and pure epoxy and polyimide resins were irradiated with 60Co -rays or 2 MeV electrons at room temperature. Mechanical tests were then carried out at 77 K and at room temperature. Following irradiation, the Young's (tensile) modulus of these composites and pure resins remains practically unchanged even at 170 MGy for both test temperatures. The ultimate strength, however, decreases appreciably with increasing dose. The dose dependence of the composite strength depends not only on the combination of fibre and matrix in the composite but also on the test temperature. A relationship is found between the composite ultimate strain and the matrix ultimate strain, thus indicating that the dose dependence of the composite strength is virtually determined by a change in the matrix ultimate strain due to irradiation. Based on this finding, we propose a mechanism of radiation-induced degradation of a polymer matrix composite in order to explain the dose dependence of the composite strength measured at 77 K and at room temperature. 相似文献
5.
6.
Yan Liu Shigenori Mitsushima Ken-ichiro Ota Nobuyuki Kamiya 《Electrochimica acta》2006,51(28):6503-6509
The electro-oxidation of dimethyl ether (DME) on PtMe/Cs (Me = Ru, Sn, Mo, Cr, Ni, Co, and W) and Pt/C electro-catalysts were investigated in an aqueous half-cell, and compared to the methanol oxidation. The addition of a second metal enhanced the tolerance of Pt to the poisonous species during the DME oxidation reaction (DOR). The PtRu/C electro-catalyst showed the best electro-catalytic activity and the highest tolerance to the poisonous species in the low over-potential range (<0.55 V, 50 °C) among the binary electro-catalysts and the Pt/C, but at the higher potential (>ca. 0.55 V, 50 °C), the Pt/C behaved better than PtRu/C. The apparent activation energy for the DOR decreased in the order: PtRu/C (57 kJ mol−1) > Pt3Sn/C (48 kJ mol−1) ≈ Pt/C (46 kJ mol−1). On the other hand, the activation energy for the MOR showed a different turn, decreased in the following order: Pt/C (43 kJ mol−1) > Pt3Sn/C (35 kJ mol−1) ≈ PtRu/C (34 kJ mol−1). The temperature dependence of the DOR was greater than that of the oxidation of methanol (MOR) on the PtRu/C. 相似文献
7.
Application of an ultrahigh-resolution scanning electron microscope (UHS-T1) to biological specimens
Keiichi Tanaka Akira Mitsushima Yuzuru Kashima Takashi Nakadera Hitoshi Osatake 《Microscopy research and technique》1989,12(2):146-154
In 1985 we developed an ultrahigh-resolution scanning electron microscope with a resolution of 0.5 nm. It is equipped with a field emission gun and an objective lens with a very short focal length. In this study we report a survey of some different preparation techniques and biological specimens using the new scanning electron microscope. Intracellular structures such as cell organelles were observed surprisingly sharper than those observed by ordinary scanning electron microscopes. However, at magnifications over 250,000 X, platinum particles could be discerned as scattered pebbles on the surface of all structures in coated materials. Using an uncoated but conductively stained specimen, we successfully observed ribosomes on a rough endoplasmic reticulum at a direct magnification of 1 million. In these images some protrusions were recognized on the ribosomes. Ferritin and immunoglobulin G were used as samples of biological macromolecules. These samples were observed without metal coating and conductive staining. The ferritin particles appeared as rounded bodies without any substructure on the surface and immunoglobulin G as complexes of three-unit bodies. In the latter the central body might correspond to the Fc fragment and two side ones to Fab fragments. We assume that ultrahigh-resolution scanning electron microscopy is an effective means for observation of the cell fine structures and biological macromolecules. It will open a new research field in biomedicine. 相似文献
8.
In order to observe intracellular structures by scanning electron microscopy, excess cytoplasmic matrix must be removed from the fractured surface of cells. Previously we reported an Osmium-DMSO-Osmium method devised for this purpose. This method is very effective in revealing intracellular structures, but requires osmium tetroxide for initial fixation with some consequent disadvantages. In the present study, a revised Osmium-DMSO-Osmium method is reported, in which an aldehyde mixture is used as the initial fixative instead of osmium tetroxide. As fixation is carried out by perfusion in this revised method, better preservation of fine structures is achieved than by the original method, especially in the central nervous tissue which tends to suffer from post-mortem degeneration. Moreover this method can be applied to cytochemical studies of intracellular structures with a scanning electron microscope (SEM). In this study, acid phosphatase of lysosomes is demonstrated in a coloured SEM micrograph. 相似文献
9.
This paper presents the design of nonlinear servo systems using the fuzzy method. The idea behind this control method is to divide the operating region of a nonlinear system into a collection of local linear servo control systems and apply the fuzzy method for calculation of the control input. The control input of each local linear servo system is calculated using the Davison–Smith method and the Hikita pole assignment method. We can prove that all states of the fuzzy servo system are bounded and that the output errors converge to zero by the Lyapunov method. Simulations on a nonlinear mass‐spring‐damper system illustrate the effectiveness of the proposed method. 相似文献
10.
Shigenori Takada Takashi Kusukawa Norio Tagawa Atsunobu Mori Yoshiaki Mizoh Masaru Nakakita 《Microsystem Technologies》2007,13(8-10):767-775
Achieving ultra-high magnetic-recording density in hard disk drives (HDDs) requires clarification of flow-induced vibration issues. In particular, it is necessary to reduce the flow-induced disk vibration called disk flutter. Thus far, however, there has been no experimental research related to disk flutter in actual HDDs. For this study, therefore, the disk-flutter issues have been studied experimentally, using an actual 2.5-in. HDD with one disk and two magnetic heads. The aim was to study the effect of operating magnetic-head mechanisms on flow-induced disk flutter. This study evaluated disk flutter as well as static pressure distribution in the actual HDD, by taking measurements while changing the operating modes of the magnetic-head mechanism as well as the number of operating air-bearing sliders. The study demonstrated that the disk-flutter amplitude increases and its frequency decreases when the magnetic-head mechanisms are operating. It was also found that the amount of decrease in the disk-flutter frequency depends on the number of operating air-bearing sliders whose amplitude increase varies with the specific operating mode of the head mechanisms, such as whether it is in track-following or seek modes. In addition, operation of the magnetic-head mechanisms generated non-uniform static pressure distribution within the HDD. These factors suggest that a decrease in disk-flutter frequency results from the slider-coupled vibration and an increase in disk-flutter amplitude results from the static pressure changes as well as air-following changes, as these vary with the actual operation of the disk head mechanism. From these experimental results, it appears that the disk-flutter issues in actual HDDs should be considered as a system that includes the operation of the magnetic-head mechanisms and disk-coupled vibration. 相似文献