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1.
Experimental results are presented to show that the adhesion force is the single most important limiting factor in high-resolution atomic force microscopy of DNA in air, prepared by the cytochrome-C-assisted spreading method. It is also shown that humidity plays a minor role in the control of probe force. Using a pure carbon film as the substrate to clean the AFM tip prior to imaging, it is demonstrated that 4-6 nm resolution on DNA can be routinely obtained by the atomic force microscope with commercial Si3N4 pyramid cantilevers. We also show that in organic solvents a resolution of up to 3 nm can be obtained under optimal conditions. 相似文献
2.
In this paper we design a dynamic dictionary for
the priced information model initiated by Charikar et al. Assume that a set S consisting of n elements is given such that
each element has an associated price, a positive real number. The cost of performing an operation on elements of S is a function
of their prices. The cost of an algorithm is the sum of the costs of
all operations it performs. The objective is to design algorithms
which incur low cost. In this model we propose a dynamic dictionary, supporting
search, insert and delete, for keys drawn from a linearly ordered set.
As an application we show that the dictionary can be used in
computing the trapezoidal map of a set of line segments, a fundamental problem in
computational geometry. 相似文献
3.
We present experimental results on the phase equilibrium of a saturated3He−4He mixture, which has been cooled to a temperature of 10–15 mK and polarized in a4He circulating dilution refrigerator to a stationary polarization of 15%, 7 times higher than the equilibrium polarization
in the external field of 7 T. The pressure dependence of the polarization enhancement in the refrigerator shows that the molar
susceptibilities of the concentrated and dilute phase of a saturared3He-4He mixture are equal atp=2.60±0.04 bar. This result affects the Fermi liquid parameters of the dilute phase. The osmotic pressure in the dilute phase
has been measured as a function of the polarization of the coexisting concentrated phase up to 15%. We find that the osmotic
pressure at low polarization (<7%) agrees well with thermodynamics using the new Fermi liquid parameters of the dilute phase. 相似文献
4.
Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro 总被引:1,自引:0,他引:1
ME Bracke BM Vyncke EA Bruyneel SJ Vermeulen GK De Bruyne NA Van Larebeke K Vleminckx FM Van Roy MM Mareel 《Canadian Metallurgical Quarterly》1993,68(2):282-289
The calcium-dependent cell-cell adhesion molecule E-cadherin has been shown to counteract invasion of epithelial neoplastic cells. Using three monoclonal antibodies, we have demonstrated the presence of E-cadherin at the surface of human MCF-7/6 mammary carcinoma cells by indirect immunofluorescence coupled to flow cytometry and by immunocytochemistry. Nevertheless, MCF-7/6 cells failed to aggregate in a medium containing 1.25 mM CaCl2, and they were invasive after confrontation with embryonic chick heart fragments in organ culture. Treatment of MCF-7/6 cells with 0.5 microgram ml-1 insulin-like growth factor I (IGF-I) led to homotypic aggregation within 5 to 10 min and inhibited invasion in vitro during at least 8 days. The effect of IGF-I on cellular aggregation was insensitive to cycloheximide. However, monoclonal antibodies that interfered with the function of either the IGF-I receptor (alpha IR3) or E-cadherin (HECD-1, MB2) blocked the effect of IGF-I on aggregation. The effects of IGF-I on aggregation and on invasion could be mimicked by 1 microgram ml-1 insulin, but not by 0.5 microgram ml-1 IGF-II. The insulin effects were presumably not mediated by the IGF-I receptor, since they could not be blocked by an antibody against this receptor (alpha IR3). Our results indicate that IGF-I activates the invasion suppressor role of E-cadherin in MCF-7/6 cells. 相似文献
5.
6.
The interactive editing and contouring of empirical fields 总被引:2,自引:0,他引:2
Dickinson R.R. Bartels R.H. Vermeulen A.H. 《Computer Graphics and Applications, IEEE》1989,9(3):34-43
A system for the interactive editing and contouring of surfaces derived from empirical fields is described. The approach taken begins with the representation of a field as a general-order, nonuniform, tensor-product, B-spline surface. It provides an interactive display for editing the surface by control-vertex manipulation and a contouring algorithm that is specifically designed for the fast and robust contouring of B-spline surfaces. Interactive editing of the resulting model is feasible because of the local nature of editing changes when B-splines are used. The use of nonuniform B-splines gives the flexibility required to model highly irregular data efficiently 相似文献
7.
Topics in Catalysis - An NO2-assisted soot oxidation based filter candidate consists of Pt-coated ceramic foam in combination with a wall-flow monolith is proposed to acquire for an optimal usage... 相似文献
8.
Effects of differences in office chair controls, seat and backrest angle design in relation to tasks
Liesbeth Groenesteijn Peter Vink Michiel de Looze Frank Krause 《Applied ergonomics》2009,40(3):362-370
In this study the influence of chair characteristics on comfort, discomfort, adjustment time and seat interface pressure is investigated during VDU and non-VDU tasks: The two investigated office chairs, both designed according to European and Dutch standards are different regarding: 1) seat cushioning and shape, 2) backrest angle and 3) controls. Thirty subjects in total, both male and female, participated in two experiments: twenty in the first and ten in the second.Significant differences are found for ease of adjustment and adjustment time of controls, independent of the tasks. Related to tasks, a significant difference was found for the backrest range of motion. For non-VDU tasks a larger range of backrest motion was preferred by 70% of the subjects. The chair design differences were most clear for comfort and adjustment time of controls, followed by comfort of backrest angle. No differences are found between seat pan comfort and discomfort, first impressions and peak interface pressure. 相似文献
9.
Groenesteijn L Ellegast RP Keller K Krause F Berger H de Looze MP 《Applied ergonomics》2012,43(2):320-328
In the present study, we investigated the effect of office tasks on posture and movements in field settings, and the comfort rating for chair characteristics and correlation with type of task. The tasks studied were: computer work, telephoning, desk work and conversation. Postures, movements, chair part inclinations and comfort rating data were collected from 12 subjects. Computer work showed the lowest physical activity, together with upright trunk and head position and low backrest inclination. Conversation shows the highest activity of head legs and low back together with the highest cervical spine extension. In contrast, desk work provoked the most cervical spine flexion and showed the second lowest activity. The telephoning tasks showed medium activity and the highest kyphosis. Conversation showed the highest backrest inclination. Positive comfort relations were found for computer work and a "swing system" chair, for telephoning and an active longitudinal seat rotation, and for desk work and a chair with a three-dimensionally moveable seat. 相似文献
10.
Michiel Hazewinkel 《Theory of Computing Systems》1976,10(1):363-385
In this paper we study real linear dynamical systems \(\dot x = Fx + Gu,y = Hx,x \in R^n \) = state space,u ∈ R m = input space,y ∈ R p = output space, under the equivalence relation induced by base change in state space; or in other words we study triples of matrices with real coefficients (F, G, H) of sizesn × n, n × m, p × n respectively, under the action(F, G, H.) →(TFT ?1,TG, HT ?1) ofGL n (R), the group of invertible realn × n matrices. One of the central questions studied is: “do there exist continuous canonical forms for this equivalence relation?”. After various trivial obstructions to the existence of such forms have been removed the answer is very roughly: no ifm ≥ 2, p ≥ 2, yes ifm = 1, orp = 1. For a precise statement cf. theorem 1.7. Existence or nonexistence of continuous canonical forms is related to the existence of a universal family of real linear dynamical systems. More precisely continuous canonical forms exist if such a universal family exists and if the underlying vector bundle of this family is the trivial vector bundle. In the case studied we show that a universal family in the appropriate sense does exist. The methods used are purely (differential) topological and in particular do not involve any algebraic geometry. There is a corresponding algebraic theory over any fieldk instead ofR which is the subject of part III of this series of papers. 相似文献