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奇异摄动系统的极限解 总被引:3,自引:0,他引:3
本文在前人工作的基础上,利用广义函数理论讨论了一般形式下的奇异摄动系统E(ε)(?)=A(ε)x+B(ε)u在v→0~+时的极限解问题,得出了这一极限解存在的充要条件。 相似文献
3.
Structure and functional complementation of engineered fragments from yeast phosphoglycerate kinase 总被引:1,自引:0,他引:1
Pecorari Frederic; Minard Philippe; Desmadril Michel; Yon Jeannine M. 《Protein engineering, design & selection : PEDS》1993,6(3):313-324
Previous studies have shown that, although the isolated structuraldomains of yeast phosphoglycerate kinase recover a quasi-nativestructure in vitro as well as in vivo, they do not reassociatenor generate a functional enzyme. The aim of this work was firstto study the folding of complementary fragments different fromstructural domains and second to determine the requirementsfor their reassociation and functional complementation. Themethod used for producing rigorously defined fragments consistsof the introduction of a unique cysteinyl residue in the proteinfollowed by a specific cleavage by 5'5'-dithiobis(2-nitrobenzoate)/potassiumcyanide at this residue. Two pairs of complementary fragmentswere thus obtained, 196/97415 and 1248/249415.The structure and stabilities of the different fragments werestudied. The short fragments, i.e. 196 and 249415were found to contain some secondary structure, but to havea low stability. Each large fragment has a high structural contentand a stability close to that of the corresponding domain. Incontrast to that observed with the isolated domains, a weakbut significant complementation was observed for the two pairsof fragments; the pair of fragments 1248/249415recovered 8% of the activity of the native enzyme upon complementation.An independent refolding of the complementary fragments beforereassociation decreased the yield of complementation for thepair of fragments 196/97415, but did not affectthe complementation for the other pair (1248/249415).From the present data and previous work on the isolated domains,it appears that the correct folding of the isolated fragmentsis not a prerequisite for their complementation. 相似文献
4.
ABSTRACTThis study focuses on the decisive role played by the digital design environment in the cognitive design process and design thinking. To analyse the cognitive role of digital design tools, we carried out a protocol analysis of conventional design sketching and a 3D sculpture tool. Cognitive evaluation was a differentiating factor when considering the contextual role of the 3D sculpture tool in subsequent evaluations, non-sequential evaluations for conversion, and passive approaches within the design process. Cognitive evaluation played the following roles: validation, extension, navigation, exploration, and confirmation. The navigation, exploration, and extension roles played by non-sequential evaluation were mainly related to inductive design thinking. Finally, the types of cognitive evaluation and their roles when using the 3D sculpture tool were different, according to the design thinking type. This study explored the multidimensional roles of cognitive evaluation using a 3D sculpture tool and its relationship with design thinking types. 相似文献
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保证所有管道中石油从一端流向另一端不泄漏要比它的探测更加困难。比较图中的3个系统,可以看到这项技术在40多年来是如何逐步发展的。 相似文献
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Chung Yon Dohn Yoo Sanghyun Kim Myoung Ho 《Knowledge and Data Engineering, IEEE Transactions on》2010,22(2):207-218
In wireless mobile computing environments, broadcasting is an effective and scalable technique to disseminate information to a massive number of clients, wherein the energy usage and latency are considered major concerns. This paper presents an indexing scheme for the energy- and latency-efficient processing of full-text searches over the wireless broadcast data stream. Although a lot of access methods and index structures have been proposed in the past for full-text searches, all of them are targeted for data in disk storage, not wireless broadcast channels. For full-text searches on a wireless broadcast stream, we firstly introduce a naive, inverted list-style indexing method, where inverted lists are placed in front of the data on the wireless channel. In order to reduce the latency overhead, we propose a two-level indexing method which adds another level of index structure to the basic inverted list-style index. In addition, we propose a replication strategy of the index list and index tree to further improve the latency performance. We analyze the performance of the proposed indexing scheme with respect to the latency and energy usage measures, and show the optimality of index replication. The correctness of the analysis is demonstrated through simulation experiments, and the effectiveness of the proposed scheme is shown by implementing a real wireless information delivery system. 相似文献
8.
通过实验对比,得出实验温度、实验时间及浓缩倍数对水处理药剂阻垢率的影响,筛选出显著影响因素,用于指导阻垢剂的选择及性能的鉴定。 相似文献
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Amruta Manke Sudjit Luanpitpong Chenbo Dong Liying Wang Xiaoqing He Lori Battelli Raymond Derk Todd A. Stueckle Dale W. Porter Tina Sager Honglei Gou Cerasela Zoica Dinu Nianqiang Wu Robert R. Mercer Yon Rojanasakul 《International journal of molecular sciences》2014,15(5):7444-7461
Given their extremely small size and light weight, carbon nanotubes (CNTs) can be readily inhaled by human lungs resulting in increased rates of pulmonary disorders, particularly fibrosis. Although the fibrogenic potential of CNTs is well established, there is a lack of consensus regarding the contribution of physicochemical attributes of CNTs on the underlying fibrotic outcome. We designed an experimentally validated in vitro fibroblast culture model aimed at investigating the effect of fiber length on single-walled CNT (SWCNT)-induced pulmonary fibrosis. The fibrogenic response to short and long SWCNTs was assessed via oxidative stress generation, collagen expression and transforming growth factor-beta (TGF-β) production as potential fibrosis biomarkers. Long SWCNTs were significantly more potent than short SWCNTs in terms of reactive oxygen species (ROS) response, collagen production and TGF-β release. Furthermore, our finding on the length-dependent in vitro fibrogenic response was validated by the in vivo lung fibrosis outcome, thus supporting the predictive value of the in vitro model. Our results also demonstrated the key role of ROS in SWCNT-induced collagen expression and TGF-β activation, indicating the potential mechanisms of length-dependent SWCNT-induced fibrosis. Together, our study provides new evidence for the role of fiber length in SWCNT-induced lung fibrosis and offers a rapid cell-based assay for fibrogenicity testing of nanomaterials with the ability to predict pulmonary fibrogenic response in vivo. 相似文献