Combating Biofilm Associated Infection In Vivo: Integration of Quorum Sensing Inhibition and Photodynamic Treatment based on Multidrug Delivered Hollow Carbon Nitride Sphere

Recently, quorum sensing (QS) inhibitors (QSIs) have been combined with antibiotics to enhance antibiofilm efficacy in vitro and in vivo. However, targeting QS signals alone is not enough to prevent bacterial infections. Drug resistance and recurrence of biofilms makes it difficult to eradicate. Herein, photodynamic therapy (PDT) is selected to unite QSIs and antibiotics. A synergistically antibiofilm system, which combines QSIs, antibiotics, and PDT based on hollow carbon nitride spheres (HCNSs) is envisaged. First, HCNS provides the multidrug delivering ability, enabling QSIs and antibiotics to be released in sequence. Subsequently, multistage releases sensitize bacteria effectively, potentiating the chemotherapeutic effects of the antibiotics. Finally, the integration of QSIs and PDT not only minimizes the possibility of drug resistance, but also overcomes the problem of limited mass and extension of PDT. Even after 48 h of incubation, the bacterial biofilm is obviously inhibited. And its biofilm disperse efficiency exceeds 48% (compared with QSI‐potentiated chemotherapy group) and 40% (compared with PDT group). Besides, the inhibition of the QS system influences phenotypes related to virulence factor production and surface hydrophobicity, which weaken biofilm invasion and formation. Eventually, this system is applied to disperse bacterial biofilm in vivo. Overall, PDT and QS modulation are devoted to eradicate drug resistance and recurrence of the biofilm.     

斜拉索-磁流变阻尼器系统半主动控制的神经网络法

磁流变阻尼器具有良好的可变阻尼特性，能用于结构的半主动控制。针对斜拉桥拉索-磁流变阻尼器系统，本文提出了利用神经控制器进行半主动控制的神经网络法，该神经控制器根据过去时刻的加速度响应和控制信号预测下一时间步的控制信号，并给出了训练方法及开展了数值仿真研究。仿真结果显示，采用神经网络半主动控制能取得很好的减振效果，证明该方法是可行的。     

桥梁颤振气动导数识别的迭代法

本文提出一种利用自由振动响应通过年顿－拉夫逊迭代同时识别出桥梁全部8个颤振气动导的方法。此法具有对迭代初值要求不高，识别结果稳定的特点，并具有一定的抗噪声干扰能力。数字仿真与实物试验结果表明本文方法有效，可行。     

压敏显色微胶囊及其涂料的研究进展

综述了无碳复写纸的结构类型和性能、压敏显色微胶囊制备及其微胶囊涂料的最新进展。揭示了微胶囊技术在无碳复写纸应用中的重要作用。     

金属型铝铸件渗漏修补工艺尝试

介绍了一种利用等离子气瞬时产生高温,使焊材与其周围母材瞬时熔化进行修补铝铸件缺陷的方法,并对修补后零件的密封性及材料的金相组织、抗拉强度及硬度等性能进行了试验.     

无铅钎料的超电势问题研究

通过对几种典型无铅钎料的电化学性能的研究,提出了无铅 钎料研究开发及应用时 应考虑因其超电势变化带来的影响问题。试验证明,含铋元素无铅钎料较传统的锡铅钎料超电势低,含铟无素无铅钎料则较锡铅钎料有较高的超电势,并证明这是由 于合金中形成了 Ｉｎ Ｓｎ４ 化合物的缘故,从 而认为含铋无铅钎料应谨慎地使用于电器元件的焊接生产中,而含铟无铅钎料 则较锡铅钎料具有更高 的电化学安全性,在电器元件的焊接生产中具有良好的应用前景。     

Regulation of cell fate by near infrared-responsive telomerase activity

Here we develop a strategy using near infrared (NIR) modulation of telomerase activity based on gold nanocage@smart polymer system. Using this biocompatible design, we can regulate cellular behavior. This system has been used in vivo by taking advantages of NIR. This is the first example for optical modulation of telomerase activity in living cells and tissues.

     

A pH-switched mesoporous nanoreactor for synergetic therapy

Zinc oxide nanoparticles (ZnO NPs),as a new type of pH-sensitive drug carrier,have received much attention.ZnO NPs are stable at physiological pH,but can dissolve quickly in the acidic tumor environment (pH ＜ 6) to generate cytotoxic zinc ions and reactive oxygen species (ROS).However,the protein corona usually causes the non-specific degradation of ZnO NPs,which has limited their application considerably.Herein,a new type of pH-sensitive nanoreactor (ZnO-DOX@F-mSiO2-FA),aimed at reducing the non-specific degradation of ZnO NPs,is presented.In the acidic tumor environment (pH ＜ 6),it can release cytotoxic zinc ions,ROS,and anticancer drugs to kill cancer cells effectively.In addition,the fluorescence emitted from fluorescein isothiocyanate (FITC)-labeled mesoporous silica (F-mSiO2) and doxorubicin (DOX) can be used to monitor the release behavior of the anticancer drug.This report provides a new method to avoid the non-specific degradation of ZnO NPs,resulting in synergetic therapy by taking advantage of ZnO NPs-induced oxidative stress and targeted drug release.     

网络管理协议SNMPv1和SNMPv2的分析

在介绍ＳＮＭＰｖ１协议的基础上讨论了ＳＮＭＰｖ２协议的特点及对ＳＮＭＰｖ１的改进,并分析了ＳＮＭＰｖ１和ＳＮＭＰｖ２的共存问题。