Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction

Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.     

综合战场态势仿真系统设计与实现

为了满足海军诸兵种一体化作战模拟系统从整体上展现综合战场态势的需求,基于高层体系结构( HLA)技术,提出了综合战场态势仿真系统的设计方案,分析了系统所需的功能,阐述了系统实现时所涉及的5个关键技术,重点解决了在态势中如何显示矢量军标的难题,并优化了数据更新流程及态势显示处理方法,极大地提高了态势显示刷新的速度与效率,最后用仿真实验验证了该设计方案的可行性与合理性.     

短基线时差法雷电定位仪的定位精度分析

甚高频短基线时差法雷电定位系统是当前雷电定位系统研究的一个热点问题.用先导通道上足够多个辐射点源代替连续的先导通道,计算了短基线时差法雷电定位系统的方位角和仰角误差.研究发现,方位角误差较小,而仰角误差较大.方位角和仰角误差随距离的增大而减小,但方位角和仰角误差随基线长度的增加而增加.闪电通道分叉时或者为曲折放电路径时,方位角和仰角误差与分支通道位置和曲折方向有关.     

微机特技扫换图形发生器

本文介绍一种新型的电视特技设备,其工作原理、设计思想和电路结构与现有的扫换特技设备均不相同。该设备不用传统的“三基波迭加法”,而采用单片微机直接产生扫换波形,因此功能齐全、硬件简单、性能稳定可靠、易于生产,并具有较高的性能价格比。本文对“微机特技”的基本设计思想、电路工作原理和结构、软件设计及关键技术都一一作了介绍。     

碳钢电极在Al_2O_3纳米流体中的腐蚀行为研究

采用电化学阻抗谱和极化曲线研究了碳钢电极在以模拟冷却水为基液的Al_2O_3纳米流体中的腐蚀行为.实验结果表明,Al_2O_3纳米颗粒对碳钢的腐蚀有一定的抑制作用;Al_2O_3纳米流体中碳钢电极的耐蚀性能随着温度的升高而降低,添加分散剂十二烷基苯磺酸钠(SDBS)对碳钢也有一定的缓蚀作用,当SDBS的用量超过一定值时,对碳钢的缓蚀性能开始下降.     

基于Python的Abaqus二次开发在层压板修理模拟中的应用

为加快复合材料层压板单面贴补参数设计的效率,利用基于Python的Abaqus二次开发技术,开发针对复合材料层压板单面贴补修理的快速建模及后处理程序.结果表明,程序能够通过输入单面贴补设计参数便捷地创建模型,并且计算结束后能通过快速后处理程序自动提取所需的数据和信息.该程序能够针对单面贴补修理母板的长度、宽度、单层厚度、层数和铺层顺序,补片的层数、直径和铺层顺序,以及复合材料单向预浸料和胶膜的性能等参数进行设计分析.用该程序计算得到的易破坏位置和破坏模式与试验结果的误差小于10%,证明该程序可行.     

1000BASE—T—在5类铜缆上实现千兆传输

１０００ＢＡＳＥ－Ｔ是一种在５类铜缆上传输１０００Ｍｂｐｓ速度的以太网解决方案。本文介绍了１０００ＢＡＳＥ－Ｔ的基本特性、关键性能参数及其设计。     

Post‐protection effect of heat‐resistant insulations on timber‐frame members exposed to fire

In lightweight walls and floors, the load‐bearing timber members are protected by cladding on the sides to form a divider between two fire compartments or to provide appropriate fire protection to the load‐bearing members. The spaces between the timber members can be void or filled with insulation materials. Although a huge number of different insulation materials exist, the most commonly used material is mineral wool insulation. The existing design model for glass wool‐insulated timber‐frame constructions, given in European standard 1995‐1‐2, assumes collapse of the glass wool after failure of the cladding. However, a new form of glass wool insulation, suitable for use at high maximum service temperatures, is now available in the market. The charring phase after the cladding's failure is known as the post‐protection phase. The behaviour of the new heat‐resistant glass wool in the post‐protection phase is similar to that of stone wool and considerably better than that of traditional glass wool. The protective properties of stone wool have changed over the last decades. Charring is one of the main parameters needed to calculate the resistance of a structure to fire. Based on experimental investigations, this paper describes the analysis of the effect of the insulation with regard to its ability to protect timber members against charring during the post‐protection phase. Copyright © 2011 John Wiley & Sons, Ltd.     

Simulation on thermoelectric device with hydrogen catalytic combustion

The micro-thermoelectric-generator based on catalytic combustion of hydrogen and oxygen was designed. With the application of general finite reaction rate model in CFD software of FLUENT, the effect of inlet parameters on the highest temperature difference between the hot and cold plate of the generator was studied. Results showed that, the temperature in the heating and cooling channel of the micro-thermoelectric-generator was uniform; with the increasing of inlet reactant temperature, the highest temperature difference increased, but the total efficiency of the generator decreased. Results can be used to the further design and optimization of micro-thermoelectric-generator based on hydrogen catalytic combustion.     

大气压下不同气体的流注放电特性

大气压下气体放电通常表现为丝状放电形式。流注或先导放电是其气体击穿的起始阶段,相对于低气压下暗放电或辉光放电具有更复杂的演化特性。为了研究不同气体的流注传播特性,采用流体模型对1 cm平板电极中大气压下氮气、氧气,以及氮气混合20%、1%和0.01%氧气的双向流注传播过程进行了仿真计算。光电离作为源项加在流体模型中,在数值仿真时采用解多组Helmholtz方程代替Zheleznyak积分计算。仿真结果表明:氧气含量较低时,流注会出现分叉现象;氧气中流注发展速度较快;氧气中正流注通道半径较大。