EFFECT OF COMMERCIAL PROCESSING STAGES OF APPLE JUICE ON PATULIN, FUMARIC ACID AND HYDROXYMETHYLFURFURAL (HMF) LEVELS

The effects of different stages of commercial apple juice production on the patulin, fumaric acid and hydroxymethylfurfural (HMF) contents of apple juice were investigated. Heat treatment and activated charcoal were effective for the reduction of patulin. The average reduction of patulin were 13.4% and 22.9% for heat treatment and activated charcoal, respectively. Statistical analysis showed no significant differences (P<0.05) in the presence of fumaric acid between different treatments. Filtration and heat treatment caused an apparent increase in the HMF content, while activated charcoal caused a decrease. The average increments of HMF were 40.4% and 38.0% for separation and heat treatment, respectively, while the average reduction of HMF was 23.7% for activated charcoal. Spiking of samples with known amounts of patulin and fumaric acid revealed respective recovery rates of 96.4% and 95.9%.     

CHARACTERIZATION OF POLYPHENOLOXIDASE FROM WILD PEAR (PYRUS ELAEGRIFOLIA)

Wild pear polyphenoloxidase (PePPO) was extracted and purified using a Sepharose 4B- l -tyrosine- p -amino benzoic acid affinity column. Optimum conditions for pH, temperature and heat inactivation were determined. At the optimum pH and temperature, K _M and V _max values for PePPO with catechol and pyrogallol were determined. The V _max/ K _M showed that PePPO has the greatest activity toward catechol. Optimum pH for PePPO was pH 6.0 using catechol as substrate. Optimum temperatures of PePPO for pyragallol and catechol were 65 and 35C, respectively. Enzyme activity decreased because of heat denaturation with increasing temperature. Inhibition of PePPO was investigated using p -aminobenzoic acid, ethyleneglycol, l -cysteine, l -tyrosine, sodium azide, p -aminobenzenesulfonamide, β-mercaptoethanol and dithiothreitol and catechol as substrate. Competitive-type inhibition was obtained with ethyleneglycol, l -cysteine, l -tyrosine, p -aminobenzenesulfonamide and dithiothreitol. Uncompetitive inhibition was obtained with β-mercaptoethanol, sodium azide and p -aminobenzoic acid. These results show that the most effective inhibitor for PePPO was dithiothreitol and that the type of inhibition depended on the origin of PPO.

PRACTICAL APPLICATIONS

In this present work, the properties of polyphenoloxidase in Pyrus elaegrifolia , including optimum temperature, optimum pH, substrate specificity and response to inhibitors, were studied.     

基于小生境遗传算法的通用涡旋型线优化研究

目前涡旋型线的优化设计多数是在一些参数修改设计的基础上,简化为单目标优化问题,因而不易获得多性能最优的设计方案,涡旋型线形状的设计本质上是一个多目标优化问题,在通用涡旋型线形状优化设计中,根据关于切向角参数的基于曲率半径的Whewell固有方程的通用涡旋型线泛函表征形式,建立了多目标涡旋型线形状优化设计数学模型,应用了基于共享小生境技术的非劣优选遗传算法,给出了通用涡旋型线多目标形状优化设计实例分析.     

Part-mix allocation between automated and conventional manufacturing

The overall part-mix of a production system is allocated optimally between the existing conventional facilities and planned automated system to be acquired. Approximate mean value analysis (MVA) is utilized to evaluate performance of the automated system under trial part-mix allocations. Cost of processing and WIPs are taken as the sources of operational costs. Cost minimizing route allocations when alternatives exist are handled by a search procedure.     

EFFECT OF ACTIVATED CHARCOAL ON WATER-SOLUBLE VITAMIN CONTENT OF APPLE JUICE

The effect of activated charcoals (0, 0.5, 1.0, 2.0 and 3.0 g/L of powdered and granular activated charcoals) on the content of several water-soluble vitamins in apple juice was studied. Apple juice samples with activated carbon added were mixed for 0 (control), 5, 10, 20, and 30 min, respectively. The content of water-soluble vitamins was analyzed by HPLC. Considerable reduction in ascorbic acid (Vit C), niacin, pyridoxine (Vitamin B₆), thiamine (Vitamin B₁) and biotin concentrations was found while there was a dramatic improvement in the color and clearness of apple juice. The highest decrement in water-soluble vitamins was obtained at 3.0 g/L powdered activated charcoal. Statistical analysis of the data showed highly significant differences (P |< 0.05, P < 0.01) in the water soluble vitamins, color and clearness of the apple juice samples between the dosages of activated charcoals but no significant differences induced by the mixing periods.